FastQCFastQC Report
Mon 6 Jun 2016
ERR523090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5884059
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236530.4019844124608539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208610.3545341744533833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131030.22268641425927238No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89520.15213987487209085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG117850.059.1835982
ACCTGGG131600.052.0620423
GTACCTG140650.050.1418041
CCTGGGG111600.043.033464
TATAACG8400.041.4046862
TAACGCA9300.036.380544
ATAACGC9700.035.3647583
CTGGGGG68750.033.6971975
TGGGGGG116200.030.4107826
GGTATCA206850.029.321261
GTATCAA290850.027.4645671
GTACCCG11600.027.1559641
ATGGGGG108250.027.1313675
GTATAAC17750.026.2231751
CATGGGG171150.026.1109814
GTACATG270300.025.7780821
TACATGG277850.024.5783252
ACATGGG278750.023.9045683
TCAACGC342250.023.0255074
ATCAACG344400.022.8544753
Produced by FastQC (version 0.11.5)