FastQCFastQC Report
Mon 6 Jun 2016
ERR523089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6279985
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT591290.9415468349048604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215350.34291483180294224No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT169700.2702235753747819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152270.2424687320113026No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC118850.18925204439182577No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC112200.17866284712463484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103430.16469784561587328No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG90880.14471372144997163No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA84310.13425191302208525No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC74370.1184238497384946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG51250.033.667982
GTACATG236200.029.6991651
TACATGG243050.028.4745622
ACATGGG247550.027.0852413
CATGGGG170750.027.0386664
ATGGGGG111950.026.7929555
GTACCTG69350.026.033951
ACCTGGG66800.025.410133
TATCACG6850.025.3954582
TGGGGGG115000.022.5490686
AGAGTAC287750.019.6552110-11
GAGTACT184600.019.60746212-13
AGTACTT194050.018.640512-13
GGGTTAG19100.017.2313791
GTACTTT208100.017.16930414-15
CCTGGGG70350.017.0416074
GAGTACA181050.016.879911
TATACCG4509.094947E-1216.7159675
CATGGGA83500.015.9343534
AGTACAT175350.015.7926292
Produced by FastQC (version 0.11.5)