FastQCFastQC Report
Mon 6 Jun 2016
ERR523089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6279985
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT597150.9508780673839189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197220.314045336095548No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT167720.26707070160199425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145320.2314018265967196No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC120980.19264377223830947No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC116970.18625840666816879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96770.15409272474376928No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG93840.14942710850424007No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA88990.14170416012140155No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA84070.13386974650417158No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC77380.1232168548173284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55550.033.67572
GTACATG228550.029.3259621
TACATGG232650.028.4659442
GTACCTG72750.026.8308051
ACATGGG237150.026.811843
CATGGGG165150.026.1794914
ATGGGGG108650.025.2164465
ACCTGGG72750.024.8702223
TATAACG5450.024.1478882
TAACGCA5750.022.8845374
TGGGGGG106550.021.391116
ATAACGC6050.020.9729883
AGAGTAC283550.019.0840510-11
GAGTACT182800.018.99324612-13
TATCACG5700.018.9657762
AGTACTT190300.018.20763612-13
GAGTACA174450.017.6060071
GTACTTT201550.017.08323714-15
CCTGGGG69050.016.9467964
GGGTTAG18200.016.2812521
Produced by FastQC (version 0.11.5)