FastQCFastQC Report
Mon 6 Jun 2016
ERR523088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15921202
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310100.19477172640608417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257870.16196641434484657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA272400.036.2059361
GTATCAA412350.032.472071
TCAACGC471700.027.668154
CAACGCA475000.027.494265
GTACATG518500.027.2392431
ATCAACG481700.027.103873
TACCTGG144500.026.7545662
AACGCAG493850.026.5468246
TATCAAC499500.026.4113942
TACATGG529750.026.1106682
ACATGGG526000.025.384473
CATGGGG326800.023.5442184
ACGCAGA567650.022.9852967
CGCAGAG584600.022.2535578
CGTATCA61550.021.52849294
CCGTATC86500.021.34857794
ACCTGGG181500.020.4709953
GCCGTAT112000.019.88624894
GCAGAGT663550.019.2932999
ATGGGGG189050.019.2292615
Produced by FastQC (version 0.11.5)