FastQCFastQC Report
Mon 6 Jun 2016
ERR523088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15921202
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT533470.33506892255999265No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT293500.184345377943198TruSeq Adapter, Index 3 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289730.18197746627421724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260970.16391350351562653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA272500.037.6811181
GTATCAA413050.031.9992831
TCAACGC479300.026.9872254
TACCTGG137700.026.9331782
CAACGCA485300.026.6346195
ATCAACG492350.026.233733
GTACATG518300.025.8733441
AACGCAG501250.025.8034136
TATCAAC507150.025.6736642
TACATGG527500.025.0307462
ACATGGG527300.024.254263
ACGCAGA586650.021.9673167
CATGGGG331350.021.8733184
CGCAGAG609450.021.0986338
ACCTGGG180200.020.110223
ATGGGGG186800.019.249025
GAGTACT325450.018.76567812-13
GCAGAGT680550.018.6457589
AGAGTAC576750.017.43421410-11
GTACCTG224400.017.418661
Produced by FastQC (version 0.11.5)