FastQCFastQC Report
Mon 6 Jun 2016
ERR523087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6867328
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT635650.9256147369107752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212680.30969832808335357No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT181680.2645570446030829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170370.24808775698495836No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC129320.18831196063447095No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA105740.1539754617807683No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG104340.15193682317198187No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA95360.13886041266705187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94920.13821969767571898No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC81610.11883806918789956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG247400.026.623541
TACATGG255550.025.1660692
ACATGGG262200.023.8650993
CATGGGG163600.022.6434064
ATGGGGG94350.020.2807065
GAGTACT186450.020.27144612-13
TACCTGG48050.019.567792
AGAGTAC309400.018.90333210-11
GTACTTT198550.018.8224214-15
AGTACTT196800.017.96397412-13
GTACCTG57550.017.3188061
TATACCG5200.017.1783245
TATAGCG2859.572304E-716.4962675
GTATAGG20650.016.39231
GAGTACA199650.016.0834351
ACTTTTT244650.015.65857416-17
AGTACAT193000.015.3701192
CATGGGA100900.015.2820444
TACTTTT238050.014.69270314-15
TATACTG31600.014.1340655
Produced by FastQC (version 0.11.5)