##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523087_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6867328
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89741424903543	34.0	31.0	34.0	30.0	34.0
2	32.20434847439936	34.0	31.0	34.0	30.0	35.0
3	32.04859036877225	34.0	31.0	34.0	28.0	35.0
4	34.6585522054575	35.0	35.0	37.0	32.0	37.0
5	34.669135652177964	37.0	34.0	37.0	32.0	37.0
6	34.8390675383497	37.0	35.0	37.0	33.0	37.0
7	34.69133176688226	37.0	35.0	37.0	32.0	37.0
8	34.8331560688524	37.0	35.0	37.0	32.0	37.0
9	36.08326382546458	39.0	35.0	39.0	32.0	39.0
10-11	36.181100567789976	38.0	35.5	39.0	32.5	39.0
12-13	36.23556629303275	38.0	35.5	39.0	32.5	39.0
14-15	37.32073398853237	39.0	36.5	41.0	32.0	41.0
16-17	37.21268454047921	39.0	36.5	41.0	32.0	41.0
18-19	37.24280462503029	39.0	36.5	41.0	32.5	41.0
20-21	37.26484973486049	39.0	36.0	41.0	32.0	41.0
22-23	37.16858034158263	39.0	36.0	41.0	32.0	41.0
24-25	37.166006196296436	39.0	36.5	41.0	32.0	41.0
26-27	36.988433128576354	39.0	36.5	41.0	32.0	41.0
28-29	36.80251948064807	39.0	36.0	41.0	31.0	41.0
30-31	36.724522332412256	38.5	36.0	41.0	30.5	41.0
32-33	36.55402334066467	38.0	35.0	41.0	30.5	41.0
34-35	36.44600541870142	38.5	35.0	41.0	30.0	41.0
36-37	36.3284457943468	38.0	35.5	40.5	30.0	41.0
38-39	36.08750790700546	38.0	35.5	40.0	30.0	41.0
40-41	36.05115548580176	38.0	35.0	40.0	30.0	41.0
42-43	36.00106875629066	38.0	35.0	40.0	29.0	41.0
44-45	35.89482641574715	38.0	35.0	40.0	29.0	41.0
46-47	35.611382549370006	38.0	35.0	40.0	28.0	41.0
48-49	35.724439330697464	37.5	35.0	40.0	28.0	41.0
50-51	34.707938080138305	37.5	34.0	39.0	26.0	40.0
52-53	34.873952795031784	37.0	34.5	39.5	26.5	40.0
54-55	35.457957665630644	37.0	35.0	40.0	28.0	41.0
56-57	35.59764132425304	37.5	35.0	40.0	28.0	41.0
58-59	35.58231396548993	38.0	35.0	40.0	27.5	41.0
60-61	35.30992949513988	37.0	35.0	40.0	27.0	41.0
62-63	35.115442789393484	37.0	34.0	40.0	26.5	41.0
64-65	34.88378886227657	37.0	34.0	39.5	26.5	41.0
66-67	34.635350313833854	37.0	34.0	39.0	26.0	41.0
68-69	34.29137278720341	36.0	34.0	39.0	26.0	41.0
70-71	33.87943731535759	36.0	34.0	38.0	25.5	40.5
72-73	33.62123623627705	36.0	33.5	37.5	25.0	40.0
74-75	33.36426445627761	35.0	33.5	37.0	24.5	39.0
76-77	33.02461495941362	35.0	33.0	37.0	24.5	39.0
78-79	32.71764214553317	35.0	33.0	37.0	23.5	38.5
80-81	32.48334781737526	35.0	32.0	36.0	23.0	38.5
82-83	32.254794295539696	35.0	32.0	36.0	22.0	38.0
84-85	31.96326031026915	35.0	32.0	36.0	21.0	37.0
86-87	31.885466880277164	35.0	32.0	36.0	20.5	37.0
88-89	31.573347231994745	35.0	31.5	35.5	19.5	37.0
90-91	31.467186873846714	35.0	31.0	35.5	19.5	36.5
92-93	31.229397955070738	35.0	31.0	35.0	19.0	36.0
94-95	31.132294248942237	34.5	31.0	35.0	18.5	36.0
96-97	30.876029585305957	34.0	30.5	35.0	19.0	36.0
98-99	30.834287076429142	34.5	30.5	35.0	18.5	36.5
100	29.587762809640083	34.0	28.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07416411840359771
1101	2	0.01803938757915091
1101	3	-0.01972676738125756
1101	4	-0.0369483962933046
1101	5	-0.03101089892056308
1101	6	0.03232184993840548
1101	7	0.10922600570403773
1101	8	0.016728797506459614
1101	9	-0.12648878151978948
1101	10-11	-0.04547387820811366
1101	12-13	0.025377561383372438
1101	14-15	0.0797974874880154
1101	16-17	-0.06483316141513029
1101	18-19	-0.126313193793969
1101	20-21	-0.050160172702923944
1101	22-23	-0.12016280755780429
1101	24-25	-0.14909770663890498
1101	26-27	-0.22756287189559288
1101	28-29	-0.17991699551008367
1101	30-31	-0.13763000102193956
1101	32-33	-0.16271207048487923
1101	34-35	-0.33262405147245033
1101	36-37	-0.21949834890997977
1101	38-39	-0.2537858632082717
1101	40-41	-0.32387337300249186
1101	42-43	-0.3087800518813566
1101	44-45	-0.29646336884394486
1101	46-47	-0.28939829367672587
1101	48-49	-0.3069535812239437
1101	50-51	-0.4230622327962621
1101	52-53	-0.4527836413284092
1101	54-55	-0.4126179396954015
1101	56-57	-0.29867174474613023
1101	58-59	-0.2486939434759563
1101	60-61	-0.19240884976304784
1101	62-63	-0.3178000933845837
1101	64-65	-0.22503663689503384
1101	66-67	-0.4656806451683835
1101	68-69	-0.3837982495625809
1101	70-71	-0.31429567969575345
1101	72-73	-0.289910997375749
1101	74-75	-0.4874116616021027
1101	76-77	-0.3424409436738287
1101	78-79	-0.5895662623183782
1101	80-81	-0.35709840904463164
1101	82-83	-0.45393460064808977
1101	84-85	-0.702004371282829
1101	86-87	-0.9728750599189873
1101	88-89	-0.5118683485560993
1101	90-91	-0.6624047098503958
1101	92-93	-0.44495952972399877
1101	94-95	-0.7220261419905256
1101	96-97	-0.6844813366038736
1101	98-99	-0.5720776823601916
1101	100	-0.7905433700519744
1102	1	-0.0637811415546814
1102	2	-0.10067759178656388
1102	3	-0.011068784004649501
1102	4	-0.06877060196295304
1102	5	-0.06445649306424173
1102	6	-0.01747113296900693
1102	7	0.15951726928320653
1102	8	0.0477122104341845
1102	9	-0.015083089382613935
1102	10-11	0.013174940343525066
1102	12-13	-0.051795054057691914
1102	14-15	-4.6887024242181496E-4
1102	16-17	-0.07439367985355005
1102	18-19	-0.028919032479862494
1102	20-21	-0.116433857957567
1102	22-23	-0.13158453979776397
1102	24-25	-0.002977380782589023
1102	26-27	-0.11615201445155066
1102	28-29	-0.5580405074426977
1102	30-31	-0.24320815414976948
1102	32-33	-0.13169641586662806
1102	34-35	-0.14611061678584036
1102	36-37	-0.1915243410880052
1102	38-39	-0.20615197856525924
1102	40-41	-0.667332975192906
1102	42-43	-0.08278254706743837
1102	44-45	-0.20585407588398397
1102	46-47	-0.28238690394283594
1102	48-49	-0.5947687705708162
1102	50-51	-0.37449551806901127
1102	52-53	-0.4901142762258104
1102	54-55	-0.2730922175069779
1102	56-57	-0.14423323970406443
1102	58-59	-0.14942840365581844
1102	60-61	-0.6510393873314584
1102	62-63	-0.19379153919400238
1102	64-65	-0.17143331662251882
1102	66-67	-0.1349529160359353
1102	68-69	-0.30583353929294077
1102	70-71	-0.04390752779033846
1102	72-73	-0.35246213336056087
1102	74-75	-0.20637761318236159
1102	76-77	-0.3773510184466815
1102	78-79	-0.2454088349146062
1102	80-81	-0.153764976608187
1102	82-83	-0.2872945900617552
1102	84-85	-0.28230721797966396
1102	86-87	-0.3574840736205651
1102	88-89	-0.18032708912465623
1102	90-91	-0.21604535969599326
1102	92-93	-0.2884550346682495
1102	94-95	-0.47734417380465644
1102	96-97	-0.38535682403961147
1102	98-99	-0.44817399385328116
1102	100	-0.74562460075148
1103	1	-0.01563538168330325
1103	2	0.003351676481912591
1103	3	-0.12178081175809297
1103	4	-0.06938090514389472
1103	5	-0.12845301514519747
1103	6	-0.13187962310741597
1103	7	0.012982147917561804
1103	8	-0.16841385648723417
1103	9	-0.20225028601353046
1103	10-11	-0.13502379695514577
1103	12-13	-0.12119666839520704
1103	14-15	-0.18067849662292446
1103	16-17	-0.16942023863606437
1103	18-19	-0.2536094258728525
1103	20-21	-0.28324880912046524
1103	22-23	-0.32757806682021595
1103	24-25	-0.3444513195113714
1103	26-27	-0.4010141644053178
1103	28-29	-0.4927876740510584
1103	30-31	-0.3720978674746789
1103	32-33	-0.4789963182373427
1103	34-35	-0.4255061658639221
1103	36-37	-0.4774097758760476
1103	38-39	-0.4785517827121595
1103	40-41	-0.3310401765536426
1103	42-43	-0.35680117671395806
1103	44-45	-0.38108199582631386
1103	46-47	-0.4101876889689464
1103	48-49	-0.3910156585688327
1103	50-51	-0.41854772511873506
1103	52-53	-0.312331323558638
1103	54-55	-0.3046139096770375
1103	56-57	-0.29342306591919254
1103	58-59	-0.2855034725116141
1103	60-61	-0.267891895183368
1103	62-63	-0.26121775908012523
1103	64-65	-0.24312327733186834
1103	66-67	-0.26020810025480756
1103	68-69	-0.2482850627566151
1103	70-71	-0.21299674450020234
1103	72-73	-0.09199982903624715
1103	74-75	-0.19565084079373207
1103	76-77	-0.3405635894852779
1103	78-79	-0.29085324441030025
1103	80-81	-0.32760536536233076
1103	82-83	-0.3597438760153011
1103	84-85	-0.5116442947561666
1103	86-87	-0.5300042621149821
1103	88-89	-0.4181346090316467
1103	90-91	-0.43918998024983225
1103	92-93	-0.4416756758075451
1103	94-95	-0.5708764329856173
1103	96-97	-0.49383409078253493
1103	98-99	-0.5471652100335724
1103	100	-0.5418116787695553
1104	1	0.002975007154095266
1104	2	0.009327579276813935
1104	3	0.06824971363580801
1104	4	0.00759254031428469
1104	5	0.041575616937358006
1104	6	0.031672115592947137
1104	7	0.05789818182710604
1104	8	-0.06151448258375325
1104	9	-0.02594468062156352
1104	10-11	-0.0849138885313323
1104	12-13	0.0029959165132922294
1104	14-15	-0.016805960691108623
1104	16-17	-0.07290503120793801
1104	18-19	-0.11403539824978282
1104	20-21	-0.08597811378204057
1104	22-23	-0.010315613621571629
1104	24-25	-0.08471896797901479
1104	26-27	-0.11222612741205751
1104	28-29	-0.1381413122982167
1104	30-31	-0.08996713262612843
1104	32-33	-0.060898404564404984
1104	34-35	-0.1351527685321301
1104	36-37	-0.2121973990617434
1104	38-39	-0.1929139777062261
1104	40-41	-0.04004214639103765
1104	42-43	-0.12428640009919434
1104	44-45	-0.17445609770799564
1104	46-47	-0.20588195886784888
1104	48-49	-0.24690813662427757
1104	50-51	-0.2390439427475073
1104	52-53	-0.08320836319062863
1104	54-55	-0.11343332802231743
1104	56-57	-0.5679668260695223
1104	58-59	-0.023394833853487285
1104	60-61	-0.07656100501527163
1104	62-63	-0.05824957974839862
1104	64-65	-0.05552540759055802
1104	66-67	-0.03276206954801353
1104	68-69	-0.09716934118674914
1104	70-71	-0.13586079170828214
1104	72-73	-0.12074947829182037
1104	74-75	-0.07473803798437473
1104	76-77	-0.2070354151419167
1104	78-79	-0.04590470676656366
1104	80-81	-0.16280395048056562
1104	82-83	-0.050682113818609764
1104	84-85	-0.21446501121011963
1104	86-87	-0.2030992196146748
1104	88-89	-0.21498776686522447
1104	90-91	-0.38181299872095664
1104	92-93	-0.6208678213997878
1104	94-95	-0.26941456988789625
1104	96-97	-0.2604058424639817
1104	98-99	-0.32552898089754834
1104	100	-0.41050400717404045
1105	1	0.03258243387825743
1105	2	0.017216360219130422
1105	3	0.06988436562173916
1105	4	0.12406720979892327
1105	5	0.03041100977765865
1105	6	0.0026523045562640846
1105	7	0.15323795786637362
1105	8	-0.003111023748324726
1105	9	-0.03541250402096807
1105	10-11	-0.04067075876250925
1105	12-13	0.016678962651923257
1105	14-15	0.05067987274164665
1105	16-17	0.018499433235028562
1105	18-19	0.01902668239198846
1105	20-21	0.0011877091969481057
1105	22-23	-0.04120462750123011
1105	24-25	-0.0022661605159299825
1105	26-27	-0.061692371382669364
1105	28-29	-0.0830161340443425
1105	30-31	0.027286456597394704
1105	32-33	-0.03468370451383862
1105	34-35	-0.13217928361908093
1105	36-37	-0.08721536932530682
1105	38-39	0.04336488805937222
1105	40-41	-0.06625298309059957
1105	42-43	-0.1869626683293859
1105	44-45	-0.23418559496104052
1105	46-47	-0.1707178289456195
1105	48-49	-0.15260532944525806
1105	50-51	-0.33719897675067756
1105	52-53	-0.20913812974195878
1105	54-55	-0.2176856957451534
1105	56-57	-0.1357864643916855
1105	58-59	-0.10996313278594982
1105	60-61	-0.16122218345095263
1105	62-63	-0.09489237317843191
1105	64-65	-0.11341348403946228
1105	66-67	-0.09631992038857362
1105	68-69	-0.05955649440839039
1105	70-71	-0.06569837188701655
1105	72-73	-0.09263967958377606
1105	74-75	-0.16232263684923254
1105	76-77	-0.17222027080598679
1105	78-79	-0.1483168240533601
1105	80-81	-0.14479079236156167
1105	82-83	-0.14190656883045705
1105	84-85	-0.11030296636115722
1105	86-87	-0.11269448814597638
1105	88-89	-0.057961104061906354
1105	90-91	-0.19388859540983816
1105	92-93	-0.20422469844863755
1105	94-95	-0.23780058682983096
1105	96-97	-0.23192393487761365
1105	98-99	-0.23759824850846556
1105	100	-0.3171160869636047
1106	1	0.05840556109060557
1106	2	-0.0025381081895332613
1106	3	0.04768417549195192
1106	4	0.011011215063234658
1106	5	-0.0341375728465394
1106	6	-0.03895140852902301
1106	7	0.11515286827761173
1106	8	0.008336761122777148
1106	9	-0.03477213819787295
1106	10-11	-0.006384942725965459
1106	12-13	0.022501853331554855
1106	14-15	0.05049044724241014
1106	16-17	0.020675800002130984
1106	18-19	-0.05819620026147021
1106	20-21	-0.11311599562297658
1106	22-23	-0.030265387972519875
1106	24-25	0.012115028701472852
1106	26-27	-0.040023914774600655
1106	28-29	-0.1278574219198063
1106	30-31	-0.047734877856413505
1106	32-33	-0.06484056291286322
1106	34-35	-0.1152097886323844
1106	36-37	-0.04391606215355637
1106	38-39	-0.11716063182757352
1106	40-41	-0.08872412277698771
1106	42-43	-0.05729151259065901
1106	44-45	-0.10765560699763199
1106	46-47	-0.07350819743967918
1106	48-49	-0.11015381931674284
1106	50-51	-0.05110036333735479
1106	52-53	-0.11169090245023483
1106	54-55	-0.09077831850230922
1106	56-57	-0.07836123730034927
1106	58-59	-0.037154840958791624
1106	60-61	-0.03594163305792364
1106	62-63	-0.07207274638452787
1106	64-65	-0.07485393373864468
1106	66-67	-0.12783471577581196
1106	68-69	-0.10170230593220708
1106	70-71	-0.05069083606094438
1106	72-73	0.10087291897263384
1106	74-75	0.06961250906886818
1106	76-77	0.016705984936912444
1106	78-79	0.022616467607697643
1106	80-81	0.0020222747688123377
1106	82-83	-0.047773988787383814
1106	84-85	-0.10447535831553623
1106	86-87	-0.05487220500993217
1106	88-89	-0.033000856175618765
1106	90-91	0.001565697565961699
1106	92-93	-0.06480075394931362
1106	94-95	-0.07307176095963186
1106	96-97	-0.050719675056257074
1106	98-99	-0.024504328807868347
1106	100	-0.03357754156539983
1107	1	-0.10036769183884786
1107	2	-0.03419266289706968
1107	3	0.029237752475424372
1107	4	0.05493888940093683
1107	5	0.04694481735919709
1107	6	0.06572917289614111
1107	7	0.14162204240226828
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2316	68-69	0.05453080356044637
2316	70-71	0.04065039445693941
2316	72-73	0.07140674710750261
2316	74-75	0.10758949699620501
2316	76-77	-1.2288352576975896
2316	78-79	0.12921497631324996
2316	80-81	-0.911411076105896
2316	82-83	0.12339493232273213
2316	84-85	-1.4158492007527705
2316	86-87	0.17515604334274215
2316	88-89	0.33555241952853976
2316	90-91	-0.5204197688268515
2316	92-93	0.3038381894181619
2316	94-95	0.24107518353397595
2316	96-97	0.36411199699359287
2316	98-99	0.35760710686496466
2316	100	0.31632257552231735
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	15887.0
1	1327.0
2	874.0
3	782.0
4	643.0
5	723.0
6	747.0
7	1168.0
8	1967.0
9	2491.0
10	3786.0
11	7066.0
12	11653.0
13	15048.0
14	17263.0
15	19300.0
16	21643.0
17	25252.0
18	29831.0
19	36011.0
20	42627.0
21	51375.0
22	59554.0
23	65380.0
24	67482.0
25	69419.0
26	72770.0
27	80080.0
28	91990.0
29	108938.0
30	132321.0
31	164720.0
32	214459.0
33	297557.0
34	465872.0
35	851172.0
36	1076115.0
37	1132081.0
38	1277527.0
39	332022.0
40	405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.397879536102195	18.373652458354282	13.065795314038011	23.16267269150551
2	15.607289575650062	21.18643795807852	39.08111491060174	24.12515755566967
3	20.38786716906604	25.340853830459064	29.716297533504378	24.554981466970517
4	12.84273589469638	16.385215867452604	38.584302967145234	32.18774527070578
5	13.294608759308032	37.98966662544543	34.423737351146755	14.291987264099781
6	30.45571241435965	36.73167620790433	17.877302488200534	14.93530888953549
7	27.10709052312934	32.623901168337284	22.3064763315641	17.962531976969277
8	25.561482733670964	33.33972683624885	20.560249074902003	20.538541355178175
9	26.032018242707604	16.765528085067963	21.07076266568175	36.131691006542674
10-11	25.04339081530038	25.361078917022507	28.482800323377145	21.112729944299964
12-13	26.52827643164969	23.511221483243112	27.83255484170086	22.12794724340634
14-15	23.229051854912484	25.151941295834966	26.825079228554106	24.79392762069844
16-17	22.271059002426473	27.988000031253723	27.061239550358252	22.679701415961553
18-19	21.92547474840139	28.059208902151532	28.253639623315674	21.7616767261314
20-21	23.3130469970038	26.19942354860905	27.90765383115204	22.57987562323511
22-23	22.69550144528608	26.15559161781548	28.127683869260565	23.02122306763787
24-25	23.298120201159882	26.214884467021477	27.98484632353967	22.50214900827897
26-27	22.79189168898267	25.747769836166906	29.079059260412617	22.381279214437807
28-29	22.097674498789708	27.47110436324407	28.195504155665347	22.235716982300875
30-31	22.854470335510708	26.14471340497369	28.05837307266784	22.942443186847765
32-33	22.86205029583141	26.44796856150831	28.20627544278303	22.483705699877245
34-35	23.52888331070605	26.251176282750766	28.02885687082056	22.191083535722623
36-37	23.109491280812	25.899454673202378	28.698848084154037	22.29220596183158
38-39	22.65582579807352	26.82319465504066	28.364382745688626	22.1565968011972
40-41	22.82115550973963	26.851637583597398	27.956237185970185	22.370969720692784
42-43	22.374144514910167	26.906573804037432	27.26556089157591	23.45372078947649
44-45	22.824753042531352	26.206409738145293	27.629599036235035	23.33923818308832
46-47	23.430683606535947	26.729645947149493	27.48545866493537	22.35421178137919
48-49	23.016884817719696	26.684984298077264	27.592303906409864	22.70582697779318
50-51	21.983347726189294	26.99140417053394	28.41281983441227	22.61242826886449
52-53	22.779113686298153	27.5156220190037	26.937710160810035	22.767554133888112
54-55	22.263162909593202	26.556973683095258	27.6163240158379	23.56353939147364
56-57	23.025181517876884	26.409230685140194	27.350210940864798	23.215376856118116
58-59	23.80697670306701	26.38642038286924	27.13136813058592	22.675234783477823
60-61	22.958270434060683	26.76727628282557	28.1876718277856	22.086781455328154
62-63	22.413565336788707	26.897568762741926	27.959827615106054	22.729038285363316
64-65	22.22607508091551	27.564602905557155	27.35134644625295	22.857975567274384
66-67	22.735167036531028	26.597608760795865	26.94510447298456	23.722119729688544
68-69	22.255891655666222	26.778470356952173	27.414723287035507	23.5509147003461
70-71	23.14389234362945	26.499823377819908	27.209870848775896	23.146413429774743
72-73	22.74937724519304	26.16196470070755	28.02716348787124	23.061494566228173
74-75	22.0338165659655	27.78457205308873	27.14624873068591	23.03536265025986
76-77	22.397532620840778	26.462562579806924	27.119983880444092	24.019920918908202
78-79	22.537749026449692	26.58667492994975	27.460332989752413	23.415243053848144
80-81	23.034844397701974	26.52434831448686	27.133076272478267	23.30773101533289
82-83	22.729003052168217	26.20365156684424	27.893601993318335	23.17374338766921
84-85	22.373330620850254	26.881291890216342	27.52951834864732	23.21585914028609
86-87	22.204843892069462	27.11527603204884	27.263350706873158	23.416529369008536
88-89	21.99460132632458	26.87506370237665	26.886951159501983	24.243383811796786
90-91	22.64172382409991	26.081034522444213	27.132312348672706	24.144929304783176
92-93	23.05372470563206	26.6075972819842	27.198093870437194	23.14058414194654
94-95	22.629017855185488	26.26883329655217	27.39607615601255	23.706072692249794
96-97	21.6442710231935	26.31399435633877	28.277363469498184	23.764371150969545
98-99	22.23381204565572	26.699219016897977	27.067082643406344	23.99988629403996
100	22.1912150716387	25.311531716855367	27.232441750249535	25.264811461256393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10079.0
1	7778.5
2	4166.5
3	2290.5
4	1488.0
5	1398.5
6	1912.5
7	3118.0
8	5018.0
9	6246.0
10	5943.5
11	5330.0
12	5433.0
13	5810.5
14	6229.5
15	6747.0
16	7212.5
17	7641.0
18	8282.0
19	8985.0
20	9670.0
21	10788.5
22	12512.5
23	14919.0
24	18044.0
25	22112.5
26	27912.5
27	35112.0
28	43099.5
29	52996.0
30	64957.5
31	76629.5
32	89552.5
33	104701.0
34	119811.0
35	133670.0
36	150957.0
37	167173.5
38	177623.5
39	187775.0
40	195002.5
41	200159.5
42	205855.0
43	213595.0
44	222823.5
45	233559.5
46	246770.5
47	291837.0
48	383215.5
49	381230.0
50	295588.5
51	256702.5
52	242194.0
53	233493.0
54	225598.5
55	212165.5
56	194043.5
57	173298.5
58	154644.0
59	136005.0
60	115512.0
61	96787.5
62	80057.0
63	62752.5
64	46818.0
65	35070.0
66	25806.5
67	19633.5
68	15096.5
69	11372.5
70	8833.5
71	6689.0
72	5042.0
73	3874.0
74	2903.5
75	2160.5
76	1554.5
77	1042.0
78	658.5
79	407.5
80	299.5
81	216.5
82	171.5
83	137.0
84	104.0
85	88.0
86	73.0
87	56.5
88	46.0
89	38.5
90	32.5
91	31.0
92	25.0
93	19.5
94	13.0
95	8.0
96	7.0
97	7.0
98	7.5
99	5.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2027280479394606
2	0.18359396842556525
3	0.3750949423123521
4	0.3698381670425528
5	0.3814292837039384
6	0.3095235876311718
7	0.3284829266928855
8	0.31798393785763546
9	0.3309875398408231
10-11	0.3033639866917672
12-13	0.3115767879443067
14-15	0.29884257749156584
16-17	0.2933236915434941
18-19	0.3160181077705914
20-21	0.32032837225773986
22-23	0.3123704008312986
24-25	0.316403992935826
26-27	0.30535894018750814
28-29	0.330492441892975
30-31	0.31258154554435147
32-33	0.312384962535647
34-35	0.3146784309705318
36-37	0.32278201944045776
38-39	0.3301648035451343
40-41	0.35656517352891837
42-43	0.32377949618832824
44-45	0.30958911530073996
46-47	0.3143071075096457
48-49	0.33641177471062983
50-51	0.32759466272762855
52-53	0.35679816079849397
54-55	0.35679087994631975
56-57	0.36242625952917934
58-59	0.34795192540679576
60-61	0.3308492036495126
62-63	0.35339800283312517
64-65	0.34838149568507576
66-67	0.33385619559747254
68-69	0.3446172951109951
70-71	0.3646105151814505
72-73	0.3691319243816518
74-75	0.35759905453766005
76-77	0.3417195159456487
78-79	0.3524587729026486
80-81	0.3656298344858437
82-83	0.3708938906078172
84-85	0.36242625952917934
86-87	0.36163264664218747
88-89	0.34919695112859034
90-91	0.3604094634769156
92-93	0.3423747926413301
94-95	0.3648871875640715
96-97	0.37379895062533786
98-99	0.3657681706771542
100	0.3875160761216007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6867328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.478486167999186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96944544863382	42.34160787793235
2	14.54202499682012	16.42623115275207
3	4.765350251499898	8.074193047950253
4	2.0593661491031963	4.652395302678825
5	1.0546585036261127	2.978275785450506
6	0.6431593755513604	2.1794800737537896
7	0.4056354302789525	1.603677252678215
8	0.27848033896525615	1.2582518377847154
9	0.19209522140398297	0.9764322574503234
>10	1.0032801213539368	9.921593107784155
>50	0.05518973640146464	2.131848016018554
>100	0.02899419248733713	2.9262741342773437
>500	0.0014459448656177203	0.5502302922890231
>1k	5.380240055158098E-4	0.538833600347721
>5k	1.0087950103201669E-4	0.5163663738777403
>10k+	2.3538550240803893E-4	2.92430988697439
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	63565	0.9256147369107752	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21268	0.30969832808335357	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	18168	0.2645570446030829	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17037	0.24808775698495836	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	12932	0.18831196063447095	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	10574	0.1539754617807683	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	10434	0.15193682317198187	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	9536	0.13886041266705187	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9492	0.13821969767571898	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	8161	0.11883806918789956	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9123408696948797E-5
2	0.0	0.0	0.0	1.4561704348474399E-5	2.9123408696948797E-5
3	0.0	0.0	0.0	1.4561704348474399E-5	2.9123408696948797E-5
4	0.0	0.0	0.0	1.4561704348474399E-5	2.9123408696948797E-5
5	0.0	0.0	0.0	1.4561704348474399E-5	2.9123408696948797E-5
6	0.0	0.0	0.0	1.4561704348474399E-5	2.9123408696948797E-5
7	0.0	0.0	0.0	1.4561704348474399E-5	2.9123408696948797E-5
8	0.0	0.0	0.0	1.4561704348474399E-5	4.36851130454232E-5
9	0.0	0.0	0.0	4.36851130454232E-5	7.2808521742372E-5
10-11	0.0	0.0	0.0	8.73702260908464E-5	8.73702260908464E-5
12-13	0.0	2.18425565227116E-5	0.0	1.09212782613558E-4	1.747404521816928E-4
14-15	0.0	6.552766956813481E-5	0.0	1.310553391362696E-4	2.18425565227116E-4
16-17	0.0	9.465107826508361E-5	0.0	1.45617043484744E-4	2.4754897392406476E-4
18-19	0.0	1.019319304393208E-4	0.0	1.893021565301672E-4	3.27638347840674E-4
20-21	0.0	1.019319304393208E-4	0.0	2.8395323479525077E-4	3.786043130603344E-4
22-23	0.0	1.019319304393208E-4	0.0	4.659745391511808E-4	4.150085739315204E-4
24-25	0.0	1.2377448696203238E-4	0.0	8.445788522115151E-4	4.2957027827999475E-4
26-27	0.0	1.310553391362696E-4	0.0	0.0014488895826732027	4.36851130454232E-4
28-29	0.0	1.310553391362696E-4	0.0	0.0036768303479897857	4.36851130454232E-4
30-31	0.0	1.310553391362696E-4	0.0	0.009901958956962591	4.441319826284692E-4
32-33	0.0	1.310553391362696E-4	0.0	0.02602176567072375	4.7325539132541793E-4
34-35	0.0	1.310553391362696E-4	0.0	0.052007127080576324	5.533447652420272E-4
36-37	0.0	1.4561704348474398E-4	0.0	0.09154215438668431	5.82468173938976E-4
38-39	0.0	1.674596000074556E-4	0.0	0.1473426054500382	6.043107304616875E-4
40-41	0.0	1.747404521816928E-4	0.0	0.20872747013103204	6.115915826359247E-4
42-43	0.0	1.8202130435592998E-4	0.0	0.2711316541164191	6.261532869843991E-4
44-45	0.0	1.893021565301672E-4	0.0	0.35172340683305064	6.55276695681348E-4
46-47	0.0	1.893021565301672E-4	0.0	0.44993482181133626	6.698384000298224E-4
48-49	0.0	1.893021565301672E-4	0.0	0.5499518881288327	6.989618087267712E-4
50-51	0.0	1.893021565301672E-4	0.0	0.6576939386031948	6.989618087267712E-4
52-53	7.280852174237199E-6	1.893021565301672E-4	0.0	0.7670960233732829	7.353660695979572E-4
54-55	1.4561704348474399E-5	2.111447130528788E-4	0.0	0.8950570003355016	7.863320348176176E-4
56-57	1.4561704348474399E-5	2.18425565227116E-4	0.0	1.0540271267077967	7.936128869918547E-4
58-59	1.4561704348474399E-5	2.18425565227116E-4	0.0	1.2243845058805987	8.081745913403292E-4
60-61	1.4561704348474399E-5	2.2570641740135317E-4	0.0	1.4065514272800135	8.154554435145664E-4
62-63	1.4561704348474399E-5	2.3298726957559038E-4	0.0	1.5970258010102327	8.591405565599896E-4
64-65	1.4561704348474399E-5	2.3298726957559038E-4	0.0	1.7958658738886508	8.591405565599896E-4
66-67	1.4561704348474399E-5	2.3298726957559038E-4	0.0	2.034568612421017	8.591405565599896E-4
68-69	1.4561704348474399E-5	2.3298726957559038E-4	0.0	2.3049649004678385	8.591405565599896E-4
70-71	1.4561704348474399E-5	2.3298726957559038E-4	0.0	2.596825140724311	8.809831130827011E-4
72-73	1.4561704348474399E-5	2.3298726957559038E-4	0.0	2.8909570068591455	9.610724869993103E-4
74-75	1.4561704348474399E-5	2.3298726957559038E-4	0.0	3.1981434409423866	9.82915043522022E-4
76-77	1.4561704348474399E-5	2.3298726957559038E-4	0.0	3.533637828279063	9.90195895696259E-4
78-79	1.4561704348474399E-5	2.3298726957559038E-4	0.0	3.932046059253323	9.974767478704962E-4
80-81	1.4561704348474399E-5	2.3298726957559038E-4	0.0	4.355449455741738	0.0010484427130901567
82-83	1.4561704348474399E-5	2.3298726957559038E-4	0.0	4.77272965555162	0.0011066895304840544
84-85	1.4561704348474399E-5	2.3298726957559038E-4	0.0	5.200669022944586	0.0011066895304840544
86-87	1.4561704348474399E-5	2.4754897392406476E-4	0.0	5.6510625384429	0.001128532087006766
88	1.4561704348474399E-5	2.4754897392406476E-4	0.0	6.014959530111275	0.0011503746435294776
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	24740	0.0	26.62354	1
TACATGG	25555	0.0	25.166069	2
ACATGGG	26220	0.0	23.865099	3
CATGGGG	16360	0.0	22.643406	4
ATGGGGG	9435	0.0	20.280706	5
GAGTACT	18645	0.0	20.271446	12-13
TACCTGG	4805	0.0	19.56779	2
AGAGTAC	30940	0.0	18.903332	10-11
GTACTTT	19855	0.0	18.82242	14-15
AGTACTT	19680	0.0	17.963974	12-13
GTACCTG	5755	0.0	17.318806	1
TATACCG	520	0.0	17.178324	5
TATAGCG	285	9.572304E-7	16.496267	5
GTATAGG	2065	0.0	16.3923	1
GAGTACA	19965	0.0	16.083435	1
ACTTTTT	24465	0.0	15.658574	16-17
AGTACAT	19300	0.0	15.370119	2
CATGGGA	10090	0.0	15.282044	4
TACTTTT	23805	0.0	14.692703	14-15
TATACTG	3160	0.0	14.134065	5
>>END_MODULE