FastQCFastQC Report
Mon 6 Jun 2016
ERR523087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6867328
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT651820.9491610128422583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198420.28893333768242907No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT185520.27014873907289705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171130.2491944465154424No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC151340.22037683360981156No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC135890.1978790003914186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91730.13357451398855566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG247050.025.7181361
TACATGG252450.024.7437522
ACATGGG254700.023.728543
CATGGGG161200.021.8069824
GAGTACT191700.021.2252412-13
TACCTGG48950.020.8364142
GTACTTT204250.019.68817514-15
ATGGGGG89500.019.008085
AGAGTAC313450.018.42509710-11
GTACCTG60200.018.124271
AGTACTT204600.017.34798212-13
GTATAGG20850.016.6914731
CATGGGA95400.016.6505014
ACTTTTT246550.016.46967916-17
GAGTACA199550.016.426691
AGTACAT192850.015.8663592
ACCTGGG66650.014.94840053
GTATAAT32750.014.9345121
GTATTAC16600.014.7320861
GTGTAGC34400.014.6283161
Produced by FastQC (version 0.11.5)