FastQCFastQC Report
Mon 6 Jun 2016
ERR523086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8143253
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227890.27985130757941573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191890.2356429304112251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99140.12174495867928947No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93180.11442601623700013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG79350.025.839562
GGTATCA267300.025.6682681
GTATCAA371250.025.3973331
TCAACGC416700.022.3339314
CAACGCA419600.022.167825
GTACATG300600.022.0582141
ATCAACG425150.021.8900383
CGTATCA22600.021.83102294
AACGCAG431950.021.666756
TATCAAC431600.021.5521412
TACATGG303100.021.2714482
ACCTGGG93550.021.2134633
GAGTACT212950.020.72557412-13
ACATGGG302400.020.4030423
CATGGGG176600.019.6520824
TATAACG7500.019.437752
ACGCAGA480400.019.3928057
GTACCTG111650.019.037941
CGCAGAG490100.019.0081678
CCGTATC31800.018.91368194
Produced by FastQC (version 0.11.5)