FastQCFastQC Report
Mon 6 Jun 2016
ERR523086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8143253
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224370.27552871070074825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205050.25180354828715257No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT155200.19058722601397746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101250.12433606078553619No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99190.12180635920313418No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT84220.10342304236402823RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG77450.027.0087492
GGTATCA296600.025.0873431
GTATCAA402750.024.1509551
TATAACG6150.022.1660732
TCAACGC448150.021.2816774
GTACATG296400.021.1688351
CAACGCA451850.021.0452545
ACCTGGG94650.021.007013
ATCAACG457000.020.9105633
GAGTACT227200.020.74759912-13
TATCAAC464050.020.6749322
AACGCAG466100.020.4394196
TACATGG304550.020.0655292
ACATGGG302050.019.3902653
GTACCTG112400.019.3746571
GTACTTT242500.019.07980214-15
ACGCAGA512350.018.5668247
CGCAGAG522700.018.1895228
AGTACTT238200.018.05321312-13
AGAGTAC402100.017.70167510-11
Produced by FastQC (version 0.11.5)