FastQCFastQC Report
Mon 6 Jun 2016
ERR523085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870714
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162670.27708725037533766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137310.23388977899451413No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80510.137138344671534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73470.12514661760051674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG63450.037.0569082
TATAACG4050.032.511262
ACCTGGG73950.030.714243
GTACCTG96850.025.2482241
GTACATG220200.024.921871
TACATGG227850.023.5074482
GTATCAA277900.023.4531821
ACATGGG225950.023.0599423
GGTATCA209650.022.7666051
CATGGGG139150.022.7099864
CCTGGGG77150.021.2116344
ATGGGGG85950.020.9526625
CCGTATC25950.020.82423894
GTATAGG15050.020.6221771
TCAACGC311200.020.5659774
CAACGCA314150.020.3857355
ATCAACG316200.020.2853873
TAACGCA6750.020.2034264
TATCAAC319450.020.123172
GAGTACT148800.020.10328912-13
Produced by FastQC (version 0.11.5)