FastQCFastQC Report
Mon 6 Jun 2016
ERR523085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870714
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT182930.311597533110964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146680.2498503589171607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127530.2172308172396066No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT91470.15580728340709493RNA PCR Primer, Index 31 (95% over 22bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82040.13974450126509314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70480.12005354033597958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62950.034.7957342
ACCTGGG73000.029.1026743
GTACATG217950.023.9327051
GTACCTG96700.023.492961
TACATGG221750.023.1470072
TATAACG3900.022.8994392
ACATGGG219350.022.606443
GGTATCA211500.022.4831871
CATGGGG135300.022.406774
GTATCAA273550.021.6990011
ATGGGGG81650.020.9539665
TGGGGGG73750.020.0096996
GAGTACT142750.019.73772812-13
CCTGGGG75850.019.7055644
TCAACGC304300.019.1684574
TAACGCA5650.019.1336024
CAACGCA306400.019.0679235
ATCAACG310100.018.8402523
TATCAAC314750.018.6224372
AACGCAG314000.018.589386
Produced by FastQC (version 0.11.5)