FastQCFastQC Report
Mon 6 Jun 2016
ERR523084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13182174
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504990.3830855214018568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403910.30640621190404554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257770.1955443768228215No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191720.14543883277523117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG208800.051.618072
ACCTGGG241050.044.1655463
GTACCTG247750.044.1415561
CCTGGGG228450.031.1392044
GTATCAA615100.026.695771
GGTATCA441950.025.2239761
GTACATG610700.024.9394741
CATGGGG363950.024.2354374
TACATGG625650.023.7354952
TCAACGC705350.022.9838284
ATGGGGG223500.022.9493625
CAACGCA708450.022.8946115
ACATGGG631100.022.859753
ATCAACG714700.022.6267763
TATCAAC719950.022.5272672
AACGCAG729600.022.3423926
TATCACG13800.022.1488862
CTGGGGG153150.021.4884195
TGGGGGG231150.021.2031616
TATAACG11100.021.1818892
Produced by FastQC (version 0.11.5)