FastQCFastQC Report
Mon 6 Jun 2016
ERR523084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13182174
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445390.3378729487260599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385870.29272106406727755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254800.19329133419115846No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167570.12711863763898124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG212700.052.5642132
GTACCTG248750.045.3271641
ACCTGGG246950.044.4140553
CCTGGGG226100.033.0880434
TATAACG13350.028.1625462
GGTATCA446800.026.2350311
GTATCAA612600.024.998461
CTGGGGG141400.023.5293965
TGGGGGG200750.023.2678436
CATGGGG343400.023.208884
GTACATG596650.023.0976961
TATCACG13900.022.3147662
TAACGCA16500.022.2146384
TACATGG613300.022.091992
ATGGGGG211700.021.8424425
ACATGGG613350.021.2073463
TCAACGC710300.021.1840084
CAACGCA717300.021.0034035
ATCAACG722550.020.8248583
TATCAAC732550.020.6512762
Produced by FastQC (version 0.11.5)