FastQCFastQC Report
Mon 6 Jun 2016
ERR523083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007198
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.29467890126866814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23740.2357034068772972No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18030.1790114754000703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.1346309265904023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG16800.050.6680262
ACCTGGG18200.046.512093
GTACCTG20850.041.500651
CCTGGGG17750.031.5292264
GTATACG600.00414925431.351031
TATAACG1452.6435373E-829.1903422
GTACCCG2203.6379788E-1227.7884161
GTACATG39200.026.392451
GTATAAC2550.025.8184991
TACATGG40250.025.7052332
CATGGGG22300.024.885244
ACATGGG39750.024.2538933
GTATCAA52900.023.646621
ATAACGC1602.0275202E-623.5144443
GTACCGG1802.1486085E-723.5132751
TGCGTAC1000.001761397123.49341694
CTGGGGG10800.022.6401625
TACCCGG2504.3291948E-1022.5738682
TACAACG1703.4327768E-622.131242
TAACGCA1503.2752796E-521.9468144
Produced by FastQC (version 0.11.5)