FastQCFastQC Report
Mon 6 Jun 2016
ERR523083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007198
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.2881260685585158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.23163270776947534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18580.18447216932519722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13960.13860234035413097No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT11640.1155681405245046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20550.056.7329642
ACCTGGG21800.051.7470553
GTACCTG23050.051.0180131
CCTGGGG18650.040.072754
TATAACG952.1122105E-839.5878562
ATAACGC1151.1363227E-732.6981433
TATACCG902.5922589E-531.3357165
GTACCCG2350.030.0246471
CTGGGGG9600.029.3772355
GGACCGT1005.3237294E-528.1993476
TACCCGG2400.027.4228382
TAACGCA1205.838785E-627.4187534
TGGGGGG13550.025.6673016
GTACATG36200.023.3893641
ATGGGGG14650.023.1007335
TACATGG37050.022.7122632
GTATAAT4350.022.7082961
TGGACCG1903.6361962E-722.264855
CATGGGG23050.022.2272854
ACATGGG35300.021.9705093
Produced by FastQC (version 0.11.5)