FastQCFastQC Report
Mon 6 Jun 2016
ERR523082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14254245
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385710.2705930759573727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320110.22457169776442035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164600.11547437272195055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA312250.036.299441
GTATCAA476050.032.809651
TACCTGG135900.031.1465452
TCAACGC550650.028.0481134
CAACGCA557450.027.6535055
ATCAACG559700.027.578373
TATCAAC569700.027.2264652
AACGCAG575450.026.8996586
ACCTGGG169200.024.0436863
ACGCAGA647500.023.723267
CGTATCA56000.023.41191994
GTACATG478100.023.1171571
CGCAGAG667900.022.9550728
TACATGG479250.022.3453792
ACATGGG488250.021.0664733
CCGTATC77050.020.79708994
GCAGAGT735050.020.6717999
GTACCTG210150.020.5446431
GAGTACT360250.020.50883712-13
GCCGTAT98500.019.7985294
Produced by FastQC (version 0.11.5)