FastQCFastQC Report
Mon 6 Jun 2016
ERR523082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14254245
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT757760.5316030417605422No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT390780.27414991113173653RNA PCR Primer, Index 39 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363660.25512399990318674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319470.22412270870887935No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT215290.1510357090115962RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG172060.12070790140060032RNA PCR Primer, Index 39 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161300.11315927290431727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA311300.036.504221
GTATCAA462750.031.3264711
TACCTGG132950.028.214252
TCAACGC540200.026.5209144
CAACGCA546600.026.2450585
ATCAACG548300.026.1205483
TATCAAC560800.025.690732
AACGCAG565950.025.4527536
ACCTGGG159950.022.8330973
GTACATG478100.022.3912731
ACGCAGA642150.022.3522387
TACATGG479100.021.7517152
CGCAGAG659600.021.7245868
ACATGGG479600.020.8456953
GAGTACT354250.020.59001212-13
ATAACGC12750.019.5386143
GCAGAGT727400.019.5380049
CATGGGG298400.019.1226434
GTACCTG205100.019.0114021
GTACTTT382200.018.67823214-15
Produced by FastQC (version 0.11.5)