FastQCFastQC Report
Mon 6 Jun 2016
ERR523081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9642063
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT554240.5748147465952048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308620.32007673046732843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236480.24525871693640666No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT160680.1666448352390977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137870.14298807215841672No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC126540.1312374748018137No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC111030.11515170560491049No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG330100.027.2176531
TACATGG343250.025.8294182
ACATGGG349000.024.65113
CATGGGG241000.023.329324
ATGGGGG141250.021.500025
GAGTACT269150.020.693512-13
AGAGTAC413050.019.48664510-11
GTACTTT288300.019.00212514-15
GAGTACA261950.017.9300941
AGTACTT287600.017.38119112-13
AGTACAT251150.017.2950672
GTATTAC22250.016.9042341
GTATAGG27300.016.188281
ACTTTTT358250.015.65269316-17
TACCTGG69750.014.8946912
CATGGGA121800.014.85940554
GTATTAG30900.014.7587221
GTATAGA31900.014.5908311
TATCACG8100.014.5090332
TACTTTT341950.014.39948414-15
Produced by FastQC (version 0.11.5)