FastQCFastQC Report
Mon 6 Jun 2016
ERR523081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9642063
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT564340.5852896833385137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281340.2917840300358958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227670.2361216681533817No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT160900.16687300217806086No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC136180.14123533521819967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129950.13477406235574274No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC114720.11897868744479267No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA97850.10148243171611718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG328450.026.8757151
TACATGG336450.025.6204642
ACATGGG343450.024.6019213
CATGGGG238550.022.7533534
ATGGGGG137800.020.9051885
GAGTACT269450.020.31773812-13
AGAGTAC417550.019.39368210-11
GTACTTT292350.018.42553514-15
AGTACTT287950.017.46134612-13
GAGTACA259850.016.6325111
TACCTGG68400.016.4229032
AGTACAT251200.016.0724352
TGGGGGG130050.016.0079676
ACTTTTT354000.015.32028316-17
TATCACG8050.015.1804852
GTATAGG28450.015.0426421
CATGGGA120350.014.6819794
TACTTTT343900.014.41295314-15
GTATAAT45300.014.0152411
GTATAAG37800.013.9344951
Produced by FastQC (version 0.11.5)