FastQCFastQC Report
Mon 6 Jun 2016
ERR523080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581372
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353020.46564131136158465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284810.3756707888756811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183220.24167129643552646No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150410.19839416928756431No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94260.12433105775577295No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88200.11633778160470164No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76150.1004435608752611No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG161950.061.907022
ACCTGGG179050.055.470843
GTACCTG189800.053.3048821
CCTGGGG146150.043.081094
CTGGGGG96600.031.2492665
TATAACG7900.029.762992
TATCACG6550.028.7178782
TGGGGGG169450.027.3173186
CATGGGG218100.025.850474
ATGGGGG143800.025.2756615
GTACATG399650.024.2914071
TAACGCA9450.023.88444
TACCGGG9950.023.6309172
TACATGG409900.023.4152472
GTACCGG12100.023.3232291
GTATCAA471000.023.3078781
GTACACG16200.022.9369091
ACATGGG409600.022.8015543
GGTATCA349700.022.7307551
GAGTACT285150.020.28452712-13
Produced by FastQC (version 0.11.5)