FastQCFastQC Report
Mon 6 Jun 2016
ERR523080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581372
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315240.4158086425517703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273380.36059436207588813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178540.23549827128915451No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129670.17103764331838617No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93730.12363197584817102No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87440.11533532452964977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG166000.062.7663732
ACCTGGG181800.056.3238953
GTACCTG195950.053.4151231
CCTGGGG144400.044.6169244
TATAACG9750.037.597542
CTGGGGG89500.035.651365
TAACGCA11150.035.402494
TATCACG7250.032.4116742
ATAACGC12700.030.7116933
TGGGGGG148900.028.52996
GTACCCG14700.028.4680041
ATGGGGG134250.025.0627125
TACCCGG15950.024.456082
CATGGGG207900.024.2083034
TACCGGG10350.023.6119822
GTACATG407500.022.5466121
GTACACG16700.022.5246411
GTATCAA481300.021.981181
GGTATCA364800.021.9762671
TACATGG418650.021.7781052
Produced by FastQC (version 0.11.5)