FastQCFastQC Report
Mon 6 Jun 2016
ERR523079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300276
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209800.252762679216932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166010.20000539741088125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88520.10664705607379803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105350.051.3997961
GTATCAA199400.043.412351
TCAACGC236850.035.9901164
ATCAACG239700.035.347833
CAACGCA241100.035.197655
TATCAAC249550.034.203412
AACGCAG251950.033.9382826
ACGCAGA290050.029.4640647
TACCTGG73400.028.8155982
CGCAGAG299750.028.44558
GTACATG257250.026.590691
TACATGG263850.025.4557742
GTACCTG87950.024.8200551
ACATGGG267800.024.3739993
GCAGAGT345350.024.33479
CATGGGG190150.023.1559524
ACCTGGG98300.020.8911823
GAGTACT198900.020.85638612-13
CAGAGTA333850.020.15671210-11
ATGGGGG112750.020.045725
Produced by FastQC (version 0.11.5)