FastQCFastQC Report
Mon 6 Jun 2016
ERR523079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300276
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195450.23547409748784254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163040.1964272031436063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88660.10681572516383793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114000.053.1554951
GTATCAA205600.040.6631931
TCAACGC248400.033.0877724
ATCAACG252400.032.5831953
CAACGCA255000.032.212955
TATCAAC261400.031.7208672
AACGCAG266600.030.9696066
TACCTGG81050.028.7243712
ACGCAGA307950.026.7955787
GTACATG269550.025.8495451
CGCAGAG320500.025.7225868
GTACCTG94450.025.3543781
TACATGG273500.024.7802122
ACATGGG278250.023.3240243
ACCTGGG98300.023.0907783
GCAGAGT362500.022.5996869
CATGGGG198700.021.5451664
GTGGTAT59800.021.1635361
GAGTACT208700.019.9319412-13
CAGAGTA352650.019.78620110-11
Produced by FastQC (version 0.11.5)