FastQCFastQC Report
Mon 6 Jun 2016
ERR523078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9054190
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235350.2599349030669778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190070.2099249076946695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106000.11707286902528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143650.045.6195031
GTATCAA248900.038.822241
TCAACGC298250.031.8743864
CAACGCA303750.031.2181535
ATCAACG304650.031.1765173
TATCAAC312300.030.4618512
AACGCAG315400.030.2090116
GTACATG306350.027.042481
ACGCAGA363250.026.2298017
CGCAGAG373900.025.4689888
TACATGG317400.025.366952
ACATGGG322050.024.340823
CATGGGG212500.022.6447454
GCAGAGT420700.022.3441589
GAGTACT227500.020.35147312-13
AGAGTAC364050.019.96283710-11
ATGGGGG129600.019.7603155
CAGAGTA406100.019.34177210-11
GTACTTT252400.018.28835714-15
AGTACTT247300.017.73416712-13
Produced by FastQC (version 0.11.5)