FastQCFastQC Report
Mon 6 Jun 2016
ERR523078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9054190
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214570.23698420289390876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183200.20233726042859715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102950.11370426288823186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143050.046.8256261
GTATCAA248200.038.26451
TCAACGC294100.031.637854
ATCAACG298600.031.193393
CAACGCA298650.031.187315
TATCAAC310900.030.1611792
AACGCAG310500.030.057116
ACGCAGA356300.026.1801367
CGCAGAG370400.025.2257758
GTACATG300800.025.1929591
TACATGG307800.024.032782
ACATGGG314550.022.8138263
GCAGAGT421000.021.9817739
CATGGGG208250.020.7380184
GAGTACT229400.020.73460812-13
AGAGTAC370000.019.52438210-11
CAGAGTA408650.019.12695710-11
GTACTTT249550.018.85185214-15
ATGGGGG122300.017.560085
AGTACTT248600.017.52618212-13
Produced by FastQC (version 0.11.5)