##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523078_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9054190
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64370341245324	34.0	31.0	34.0	30.0	35.0
2	32.71360828522485	34.0	31.0	34.0	31.0	34.0
3	32.985650842317206	34.0	31.0	34.0	31.0	36.0
4	35.769417695011924	37.0	35.0	37.0	33.0	37.0
5	35.82726306825901	37.0	35.0	37.0	35.0	37.0
6	35.904519233636584	37.0	35.0	37.0	35.0	37.0
7	35.73257906008158	37.0	35.0	37.0	35.0	37.0
8	35.78074029813821	37.0	36.0	37.0	33.0	37.0
9	36.9970009465231	39.0	36.0	39.0	34.0	39.0
10-11	37.11783549936548	38.5	36.0	39.0	34.0	39.0
12-13	36.987939340791385	38.0	36.5	39.0	33.5	39.0
14-15	38.07894748177363	39.0	36.5	41.0	34.5	41.0
16-17	38.10119972079225	39.0	37.0	41.0	34.5	41.0
18-19	38.10597585206408	39.0	37.0	41.0	34.0	41.0
20-21	37.996420220914295	39.0	36.5	41.0	34.0	41.0
22-23	37.9144924062782	39.0	36.5	41.0	34.0	41.0
24-25	37.77926932171735	39.0	36.0	41.0	34.0	41.0
26-27	37.654107269672934	39.0	36.5	41.0	34.0	41.0
28-29	37.61937058974905	39.0	36.5	41.0	34.5	41.0
30-31	37.47202913789086	38.0	36.0	41.0	34.0	41.0
32-33	37.334134306878916	38.0	36.5	41.0	33.0	41.0
34-35	37.162734601328225	38.0	36.0	41.0	32.5	41.0
36-37	37.18538069114962	38.0	35.5	41.0	33.0	41.0
38-39	37.3284005526723	38.0	36.0	41.0	33.0	41.0
40-41	37.2341028297396	38.0	36.5	41.0	33.0	41.0
42-43	37.270425791815725	38.0	36.5	41.0	33.0	41.0
44-45	37.11516237233811	38.0	36.0	41.0	33.0	41.0
46-47	36.86527486169386	38.0	36.0	41.0	32.0	41.0
48-49	36.842134359893045	38.0	36.5	41.0	32.0	41.0
50-51	36.460005975134166	38.0	35.0	40.0	31.0	41.0
52-53	36.51743203975176	38.0	35.5	40.0	31.0	41.0
54-55	36.327166814480364	37.5	35.5	40.0	31.0	41.0
56-57	36.03501864882446	38.0	35.0	40.0	29.5	41.0
58-59	35.912596157138296	36.5	35.0	40.0	30.0	41.0
60-61	35.64881883415303	36.5	35.0	40.0	29.0	41.0
62-63	35.469692484915825	37.5	35.0	40.0	28.5	41.0
64-65	35.336658883897954	37.0	35.0	39.0	29.0	41.0
66-67	35.115467479697244	36.5	34.5	39.0	28.5	41.0
68-69	34.65268207316171	36.0	34.0	38.5	27.0	40.0
70-71	34.40648108776158	36.0	34.0	38.0	27.0	40.0
72-73	34.14422709264993	36.5	34.0	37.0	26.0	39.5
74-75	33.86067422927948	35.5	33.5	37.5	26.0	39.0
76-77	32.36509301218552	34.5	31.0	36.5	25.0	37.5
78-79	33.37292822439114	35.0	33.0	37.0	26.0	38.0
80-81	33.362737693819106	35.0	33.0	36.5	26.0	37.0
82-83	33.17175191817269	35.0	33.0	36.0	26.0	37.5
84-85	32.96068046948429	35.0	33.0	36.5	25.5	37.0
86-87	32.70389874743075	35.0	33.0	36.0	25.5	37.0
88-89	32.533533038294976	35.0	33.0	36.0	25.0	36.5
90-91	32.38382533390618	35.0	32.5	35.5	25.0	37.0
92-93	32.22521710942668	35.0	32.0	35.5	24.0	36.5
94-95	32.100659639349296	35.0	32.0	35.0	24.5	36.0
96-97	31.91719016278651	35.0	32.0	35.0	23.5	36.5
98-99	31.78338183758017	35.0	31.5	35.0	23.0	36.5
100	30.576369945848274	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.04711839715256616
1101	2	-0.1068871050972291
1101	3	-0.10397928228277209
1101	4	-0.10705211726658348
1101	5	-0.10000345644602504
1101	6	-0.16009468601398424
1101	7	-0.10683450408843953
1101	8	-0.04900636776955736
1101	9	-0.11096921094777912
1101	10-11	-0.0643424477760206
1101	12-13	-0.11722758806617861
1101	14-15	-0.2183121452517227
1101	16-17	-0.08179394062174339
1101	18-19	-0.09275283090730113
1101	20-21	-0.16344819034362246
1101	22-23	-0.08926211154062713
1101	24-25	-0.21167762567277748
1101	26-27	-0.21259267893164946
1101	28-29	-0.24286714661734976
1101	30-31	-0.25233175499765537
1101	32-33	-0.11953728332114366
1101	34-35	-0.1235983391596136
1101	36-37	-0.3082574141664267
1101	38-39	-0.1998325318754226
1101	40-41	-0.28586285720398763
1101	42-43	-0.23525578738233577
1101	44-45	-0.296284346180002
1101	46-47	-0.27840802782423424
1101	48-49	-0.4095854047293983
1101	50-51	-0.3141688156312412
1101	52-53	-0.3955156313914827
1101	54-55	-0.36104481436650104
1101	56-57	-0.4164739891420979
1101	58-59	-0.5114338267181822
1101	60-61	-0.44410090200633334
1101	62-63	-0.3007280221207296
1101	64-65	-0.29465909352670394
1101	66-67	-0.39864053588730286
1101	68-69	-0.4005036611310544
1101	70-71	-0.42216152935857565
1101	72-73	-0.6121204492411465
1101	74-75	-0.553493865663043
1101	76-77	-0.47292622631060866
1101	78-79	-0.5136317361820488
1101	80-81	-0.3658053750720711
1101	82-83	-0.4016422329130833
1101	84-85	-0.5111040506287452
1101	86-87	-0.5818800367344963
1101	88-89	-0.4771056843428312
1101	90-91	-0.5312031547896794
1101	92-93	-0.8269401156614506
1101	94-95	-0.7296083774971649
1101	96-97	-0.6273113116586053
1101	98-99	-0.5672877781789154
1101	100	-0.6097678036413683
1102	1	-0.0034654931376039144
1102	2	-0.0738115382781146
1102	3	-0.04414596067996257
1102	4	-0.03252585026252319
1102	5	-0.06417552867993237
1102	6	-0.10805320723678591
1102	7	-0.1131002275366626
1102	8	-0.04635998804070596
1102	9	-0.08613459221209752
1102	10-11	-0.09145061900162688
1102	12-13	-0.08947154915676236
1102	14-15	-0.18623751780581443
1102	16-17	-0.08684344637728003
1102	18-19	-0.11930684308789807
1102	20-21	-0.19169766062810112
1102	22-23	-0.13357354234296537
1102	24-25	-0.1400804269715863
1102	26-27	-0.13558757958600864
1102	28-29	-0.16103183446822555
1102	30-31	-0.1445370028338857
1102	32-33	-0.11537670106729792
1102	34-35	-0.10417937083379059
1102	36-37	-0.208106763808658
1102	38-39	-0.17314578272922887
1102	40-41	-0.23789720873791964
1102	42-43	-0.2036581516451932
1102	44-45	-0.28641157700079134
1102	46-47	-0.19408045464167856
1102	48-49	-0.22638365648395364
1102	50-51	-0.06596183184756654
1102	52-53	-0.15881699842469033
1102	54-55	-0.21697560819910677
1102	56-57	-0.11872355547284741
1102	58-59	-0.25468773215528273
1102	60-61	-0.15427586121842296
1102	62-63	-0.13932594472623805
1102	64-65	-0.1957993762229009
1102	66-67	-0.25761988415997905
1102	68-69	-0.19177620564329345
1102	70-71	-0.13538660566835858
1102	72-73	-0.26680107886765825
1102	74-75	-0.6533174123978185
1102	76-77	-0.18076189977665535
1102	78-79	-0.2620001310633384
1102	80-81	-0.15491851732753048
1102	82-83	-0.13684619022279776
1102	84-85	-0.12427466260358244
1102	86-87	-0.19656722698208995
1102	88-89	-0.13543500516102114
1102	90-91	-0.1691214737867952
1102	92-93	-0.2717754179010292
1102	94-95	-0.358356322856487
1102	96-97	-0.3727423421766751
1102	98-99	-0.5736908376338405
1102	100	-0.3646662524940609
1103	1	-0.1239431731836973
1103	2	-0.07768774147739066
1103	3	-0.11192444996904527
1103	4	-0.05053021123667634
1103	5	-0.1781957960099163
1103	6	-0.10794682867052785
1103	7	-0.06053061572045948
1103	8	-0.07767589339193393
1103	9	-0.08078741564048642
1103	10-11	-0.08946275968551731
1103	12-13	-0.1341158956208659
1103	14-15	-0.2098351459268386
1103	16-17	-0.17550444857179315
1103	18-19	-0.16316298604478163
1103	20-21	-0.20643758219144104
1103	22-23	-0.10328346405649569
1103	24-25	-0.171997938176645
1103	26-27	-0.2179495060257537
1103	28-29	-0.18046362770450486
1103	30-31	-0.1492000543202181
1103	32-33	-0.09578530268199614
1103	34-35	-0.04139182171141442
1103	36-37	-0.115336920575551
1103	38-39	-0.1629306180294563
1103	40-41	-0.22803971026247893
1103	42-43	-0.24899637083318282
1103	44-45	-0.33696092854907533
1103	46-47	-0.29727651635695906
1103	48-49	-0.3180944013756175
1103	50-51	-0.17310937986388097
1103	52-53	-0.1650327343559823
1103	54-55	-0.1596902280755188
1103	56-57	-0.031244690690797938
1103	58-59	-0.17371734329314137
1103	60-61	-0.1321590162744073
1103	62-63	-0.14145064397926177
1103	64-65	-0.26769154307024223
1103	66-67	-0.2818350077804297
1103	68-69	-0.11939322529880769
1103	70-71	-0.22432713134656979
1103	72-73	-0.24913728845064043
1103	74-75	-0.17023297208583443
1103	76-77	-0.12159314905490959
1103	78-79	-0.18935452397096242
1103	80-81	-0.13488744324816082
1103	82-83	-0.1698849533986504
1103	84-85	-0.10381148465656054
1103	86-87	-0.06931693984947884
1103	88-89	-0.11811223289678452
1103	90-91	-0.06782436148494497
1103	92-93	-0.1243925270756776
1103	94-95	0.014732269651140939
1103	96-97	-0.74396027082933
1103	98-99	-1.0721180611174574
1103	100	-0.32431184924200807
1104	1	-0.01759558055386634
1104	2	-0.06527417772500144
1104	3	-0.09228981935442704
1104	4	-0.07895929226989296
1104	5	-0.14455319481623974
1104	6	-0.1057660281445365
1104	7	-0.16365723260562248
1104	8	-0.054282312082349904
1104	9	-0.08123226603307643
1104	10-11	-0.11740950969866049
1104	12-13	-0.13232962009339388
1104	14-15	-0.18622109624087813
1104	16-17	-0.07603881594896933
1104	18-19	-0.13830462468376936
1104	20-21	-0.2361900504939669
1104	22-23	-0.17546947173044458
1104	24-25	-0.19980793658309182
1104	26-27	-0.13778145433199285
1104	28-29	-0.20378656279789453
1104	30-31	-0.21466501279348904
1104	32-33	-0.12935728274691627
1104	34-35	-0.10524762194638981
1104	36-37	-0.16172248353959162
1104	38-39	-0.18676839745831586
1104	40-41	-0.17764025809177753
1104	42-43	-0.1679691748497376
1104	44-45	-0.23980727864844198
1104	46-47	-0.11148326471634817
1104	48-49	-0.17670031470475323
1104	50-51	-0.0867043661099487
1104	52-53	-0.11367948300437547
1104	54-55	-0.1766748227849959
1104	56-57	-0.1475518443828463
1104	58-59	-0.12810868800773534
1104	60-61	-0.15564170815213885
1104	62-63	-0.10910644296991023
1104	64-65	-0.19328840826482008
1104	66-67	-0.27266552530008425
1104	68-69	-0.18090124304224986
1104	70-71	-0.21417956504851077
1104	72-73	-0.20845901653737542
1104	74-75	-0.22894236104824728
1104	76-77	-0.2636312552626592
1104	78-79	-0.17777298499130723
1104	80-81	-0.04167987121812189
1104	82-83	-0.02651728841264145
1104	84-85	-0.0326825862197353
1104	86-87	-0.0764270449879163
1104	88-89	-0.14144798766931643
1104	90-91	-0.13122873841943772
1104	92-93	-0.23514361677349171
1104	94-95	-0.24848090590832328
1104	96-97	0.020480382736476344
1104	98-99	-0.9594208889329607
1104	100	-0.21675554400700392
1105	1	-0.10434282480908763
1105	2	-1.039826850656496
1105	3	-0.16381396637660117
1105	4	-0.11331026835335933
1105	5	-0.12718893970736644
1105	6	-0.07855589142425856
1105	7	-0.08077332771003398
1105	8	-0.0911949488519781
1105	9	-0.13454909166095064
1105	10-11	-0.03501090407163332
1105	12-13	-0.010566670011144197
1105	14-15	-0.06828248552985627
1105	16-17	-0.5494201281721445
1105	18-19	-0.03813552042878143
1105	20-21	-0.1453569278063611
1105	22-23	-0.12420706525663405
1105	24-25	-0.13119918497552163
1105	26-27	-0.12572623482591183
1105	28-29	-0.14285954960984526
1105	30-31	-0.06366799704308335
1105	32-33	-0.06213660509045127
1105	34-35	-0.03939556912743569
1105	36-37	-0.09927569193863661
1105	38-39	-0.14614167960168345
1105	40-41	-0.08309182046234298
1105	42-43	-0.15001246590535544
1105	44-45	-0.21206452369129636
1105	46-47	-0.1270823060645725
1105	48-49	-0.09281902263030872
1105	50-51	-0.067202959973379
1105	52-53	-0.11090851220435383
1105	54-55	-0.20362511222444368
1105	56-57	-0.0012684593242866526
1105	58-59	-0.14013205625128222
1105	60-61	-0.13018392511805388
1105	62-63	0.03049152235516317
1105	64-65	0.027236084847260145
1105	66-67	-0.03715913449181585
1105	68-69	-0.043128988524408385
1105	70-71	-0.1427748602608716
1105	72-73	-0.11544808921381389
1105	74-75	-0.13673118788992866
1105	76-77	-0.03506174888803315
1105	78-79	-0.0822245016710923
1105	80-81	0.04015369250261358
1105	82-83	0.08937671038209771
1105	84-85	0.05283896955468492
1105	86-87	0.03414271762147081
1105	88-89	0.010712934779874672
1105	90-91	-0.08901380772798717
1105	92-93	-0.1302789062831522
1105	94-95	-0.1689005900496312
1105	96-97	-0.04484885908339464
1105	98-99	-0.06670759283202443
1105	100	-0.0015367410975279938
1106	1	-0.0904090939443023
1106	2	-0.10430820966774945
1106	3	-0.06322521243107815
1106	4	-0.06089872233052063
1106	5	-0.07024298938784312
1106	6	-0.05634952532471971
1106	7	-0.05160925617081347
1106	8	-0.018960200227617463
1106	9	-0.05277464477775595
1106	10-11	-0.06315030384922693
1106	12-13	-0.0499193317362554
1106	14-15	-0.046956982975231654
1106	16-17	0.053971412328770896
1106	18-19	-0.039464428690706654
1106	20-21	0.0017539738030336594
1106	22-23	0.04470708939515333
1106	24-25	0.03837611569237964
1106	26-27	0.034780636367131024
1106	28-29	-0.009761516799606795
1106	30-31	0.06440518662697059
1106	32-33	0.12801787274447207
1106	34-35	0.12486895365577766
1106	36-37	0.07388587428754789
1106	38-39	0.018985313363252487
1106	40-41	0.006093794303531297
1106	42-43	0.03677903495199075
1106	44-45	0.013810317983910636
1106	46-47	-0.007180447376086363
1106	48-49	-0.012282996978214555
1106	50-51	0.11929092061338764
1106	52-53	0.06044810742031359
1106	54-55	0.0228103479099957
1106	56-57	0.09170841405443042
1106	58-59	-0.028094167488468713
1106	60-61	0.0658124389640875
1106	62-63	0.053618645052004865
1106	64-65	0.005612546214649683
1106	66-67	0.0327547728279427
1106	68-69	0.05940191623925983
1106	70-71	0.031509618343051216
1106	72-73	-0.044617326548660685
1106	74-75	-0.008083015945636873
1106	76-77	-0.042753684611447795
1106	78-79	-2.7601492634232727E-4
1106	80-81	-0.008932604950544487
1106	82-83	0.05729367080447645
1106	84-85	0.055044668894808524
1106	86-87	0.09824281792401024
1106	88-89	0.042074548496152886
1106	90-91	0.08242662154962943
1106	92-93	0.025428299943044408
1106	94-95	-0.016897897672166096
1106	96-97	-0.07322550144929707
1106	98-99	-0.12302939176555583
1106	100	0.004317264712817348
1107	1	-0.007388882255604301
1107	2	-0.08297428430652332
1107	3	-0.03179802850884528
1107	4	-0.05378912189173235
1107	5	-0.03860963940254436
1107	6	-0.09967023131137154
1107	7	-0.028095049721009957
1107	8	-0.025361808790442808
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2316	68-69	0.12652578524481584
2316	70-71	0.1136873663067135
2316	72-73	0.12456737411537233
2316	74-75	0.06682560784805247
2316	76-77	-0.05697940320975192
2316	78-79	-0.11438878532794661
2316	80-81	-0.08144766561308359
2316	82-83	-0.054066266659326345
2316	84-85	0.08168586258346977
2316	86-87	-0.0795122227792362
2316	88-89	-0.025421130333477038
2316	90-91	-0.04056993289152189
2316	92-93	-0.19261151857412528
2316	94-95	-0.0947078706498452
2316	96-97	0.036595941795418696
2316	98-99	0.11294791150267613
2316	100	-0.1311559862899756
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	0.0
12	28.0
13	156.0
14	627.0
15	1760.0
16	4150.0
17	7884.0
18	13154.0
19	20027.0
20	28170.0
21	39420.0
22	51052.0
23	58278.0
24	63189.0
25	68920.0
26	80446.0
27	95121.0
28	116169.0
29	143509.0
30	179036.0
31	226013.0
32	289544.0
33	388889.0
34	589884.0
35	1127175.0
36	1934520.0
37	1487378.0
38	1680344.0
39	359166.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.132069939376485	19.317469852031262	13.73191599700335	23.8185442115889
2	15.793049729066173	20.955322087006774	39.61366161297122	23.637966570955836
3	18.717641159539383	25.775419320207554	30.261101796565914	25.24583772368715
4	13.049465402028918	17.319777026915833	38.05046217843744	31.580295392617803
5	13.57523980980887	37.615288185949936	34.79399826596937	14.015473738271822
6	30.762513873562792	37.07126424949783	18.181317152655083	13.984904724284306
7	27.055396451808498	32.59632280745158	22.434773292806977	17.913507447932947
8	25.58363535857294	35.18675127607159	20.71805706882641	18.51155629652905
9	26.323630567423606	16.014655317517153	20.468130328197294	37.19358378686194
10-11	24.526360119056495	26.187751327037684	28.79029253752048	20.495596016385345
12-13	25.245094005762418	23.887483337804564	28.594602558663794	22.27282009776922
14-15	22.708359636382234	25.481697882931797	26.263062743030506	25.54687973765546
16-17	22.35788161569872	28.336180481548173	26.843589675338954	22.462348227414157
18-19	22.16244317331821	27.473462382079962	28.649124373610828	21.714970070991
20-21	22.982398378823625	26.654122533207364	28.574741174394198	21.788737913574813
22-23	22.719730868525875	26.531465852132868	28.331109733821357	22.417693545519896
24-25	22.497715816983717	26.63824864115616	28.43536017472723	22.428675367132893
26-27	22.435471197277888	26.795433042095958	28.39301560470065	22.376080155925507
28-29	22.348922630787072	26.94399177861781	28.408287412151733	22.298798178443384
30-31	22.817126523655688	26.662721498066862	28.383194244508957	22.136957733768497
32-33	22.571560329711296	26.8032253165256	28.268960470542755	22.356253883220344
34-35	22.76451476977334	26.898173638789036	28.24066040814929	22.096651183288333
36-37	22.73702072086615	26.768081780065916	28.196171250787444	22.298726248280495
38-39	22.588276498219088	26.926306929111238	28.178680968678275	22.306735603991395
40-41	22.743290213531022	26.710427630309137	28.242366935691187	22.303915220468653
42-43	22.551924932449985	26.82525862510758	28.030765740611475	22.592050701830964
44-45	22.475007970466116	26.932200865355426	28.085061896820168	22.50772926735829
46-47	22.49326751700747	26.798256604908676	27.8566581090567	22.85181776902716
48-49	22.522035373692763	26.919328273215438	27.844156690590104	22.7144796625017
50-51	22.559237261540673	27.03659654263319	27.751556148658135	22.652610047168004
52-53	22.566238500574805	27.106412815557658	27.582212440365577	22.745136243501957
54-55	22.42507235270446	26.940846421303004	27.765689617763122	22.868391608229413
56-57	22.486990228810274	27.046700207776396	27.53943466088445	22.926874902528883
58-59	22.440197523042755	26.98572034622309	27.692253232325275	22.88182889840888
60-61	22.573791754285534	26.99870144228597	27.730801044545377	22.696705758883116
62-63	22.41654387412967	27.010282542673096	27.723003893685906	22.850169689511326
64-65	22.343573365791	26.81578931382192	27.656896048032632	23.183741272354453
66-67	22.27829361247829	26.926775697724324	27.52845337728329	23.2664773125141
68-69	22.22673551665102	26.96926050698625	27.54247528580107	23.26152869056166
70-71	22.276160027125673	26.802133054418952	27.48981751743192	23.431889401023454
72-73	22.222439444107106	26.97505363125499	27.298036801989454	23.504470122648446
74-75	22.23270497736592	27.05293266074582	27.236138182459623	23.478224179428636
76-77	22.16309325748842	26.896401278502246	27.29672987843183	23.6437755855775
78-79	22.124965767074066	26.85100654328657	27.32573625159029	23.698291438049075
80-81	22.148909616032427	27.057548196131055	27.018902934013685	23.774639253822833
82-83	22.275917003385	26.96209301113225	27.080233433502066	23.681756551980683
84-85	22.281104663636256	26.954371909447257	27.001516273641418	23.76300715327507
86-87	22.07165697314019	27.000055720818928	27.096835438529194	23.831451867511685
88-89	22.04656353820598	26.82025672404043	27.134468659433097	23.998711078320493
90-91	22.270186407949065	26.761248931215476	27.009545461398204	23.959019199437247
92-93	22.20248726703305	26.761422063208663	26.957376307196878	24.078714362561406
94-95	22.047131125021473	26.698967388240032	26.979592240205953	24.274309246532543
96-97	22.02248206151815	26.58360659865973	27.095191099069194	24.298720240752925
98-99	21.98989628667163	26.54496145804145	26.942596775157313	24.522545480129608
100	21.990568774013518	26.542835159868496	27.01034289202026	24.45625317409773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1295.0
1	935.0
2	856.5
3	1115.0
4	909.5
5	866.5
6	1347.0
7	2464.0
8	4016.0
9	5041.5
10	4839.5
11	4454.0
12	4814.0
13	5416.0
14	6009.0
15	6630.0
16	7314.5
17	8111.0
18	8977.5
19	9971.5
20	11201.0
21	12823.0
22	15198.5
23	18775.0
24	23689.5
25	30209.5
26	39254.0
27	50586.5
28	63554.5
29	78649.5
30	94390.0
31	110264.5
32	128795.0
33	149049.0
34	168721.5
35	188346.5
36	211780.5
37	233998.0
38	251168.0
39	266759.0
40	278374.0
41	289549.5
42	302087.0
43	315064.5
44	328064.0
45	340916.0
46	349410.0
47	355960.0
48	366664.5
49	371446.5
50	367612.0
51	359259.0
52	346218.5
53	330554.5
54	312859.5
55	288765.0
56	259911.0
57	230685.0
58	202646.0
59	172707.5
60	142456.5
61	116667.0
62	94070.5
63	73164.5
64	54925.5
65	41384.5
66	30912.5
67	23517.0
68	18152.5
69	13907.0
70	10875.0
71	8396.5
72	6672.0
73	5348.0
74	4120.0
75	3035.5
76	2067.5
77	1308.5
78	833.0
79	533.0
80	373.0
81	253.5
82	162.0
83	106.5
84	76.0
85	55.5
86	39.5
87	31.5
88	22.0
89	8.5
90	4.5
91	2.5
92	0.5
93	1.0
94	1.0
95	1.5
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030383722895145782
2	0.07893582970978077
3	0.0027611525713509433
4	3.313383085621133E-5
5	0.0015462454399565284
6	0.0010934164182549737
7	0.0
8	3.865613599891321E-4
9	2.2089220570807548E-5
10-11	1.1044610285403774E-5
12-13	1.214907131394415E-4
14-15	0.01167415307167179
16-17	0.001115505638825781
18-19	1.6014684913835472E-4
20-21	2.650706468496906E-4
22-23	0.0019493737153737662
24-25	8.559572971187925E-4
26-27	0.0070243721415168
28-29	0.008001820151775033
30-31	0.010188652988284981
32-33	0.009001357382604075
34-35	0.004362621062734491
36-37	0.003815912853607004
38-39	0.002932344030774702
40-41	0.003385173052476257
42-43	0.0017450484250937964
44-45	0.005008730764430612
46-47	0.017930924798353027
48-49	0.01877583748518642
50-51	0.013595915261332045
52-53	0.007664959538070219
54-55	0.0011652063851100983
56-57	0.0024960819245012527
58-59	0.0028605540639195775
60-61	0.0033023384753357284
62-63	0.0033244276959065364
64-65	0.003810390548464302
66-67	0.002827420233063366
68-69	0.007620781096928604
70-71	4.141728857026415E-4
72-73	0.005163355308426264
74-75	0.007692571063783729
76-77	0.006930492954090868
78-79	0.0044896340810166345
80-81	4.4178441141615095E-4
82-83	0.0014799777782441058
84-85	9.443141794020227E-4
86-87	0.0013640093702473662
88-89	0.0012149071313944152
90-91	0.0010658048925414642
92-93	3.9208366513183404E-4
94-95	0.0012093848262517133
96-97	0.002877120979347683
98-99	0.0032139815930524985
100	0.004815450084436046
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9054190.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.10704484024415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78244181391773	41.5639016824986
2	15.775423898429997	18.017756798830018
3	5.232845781498414	8.96497076058337
4	2.3281278927268545	5.318100158551024
5	1.1699977359786533	3.3407556585758544
6	0.6813723767675319	2.3346697723780307
7	0.44021064913068514	1.7597390495341314
8	0.3020101913927074	1.3797527633659248
9	0.2227550760733461	1.1448795705941317
>10	0.9842688334765256	9.71804084514208
>50	0.05266481013517876	2.0417678574634466
>100	0.026014767156142243	2.6760579492621157
>500	0.0013447579038383176	0.5263560781085331
>1k	4.390866631909076E-4	0.4285307543682848
>5k	0.0	0.0
>10k+	8.23287491649836E-5	0.7847203007445047
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21457	0.23698420289390876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18320	0.20233726042859715	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10295	0.11370426288823186	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1044610285403774E-5	0.0	0.0	0.0
3	0.0	1.1044610285403774E-5	0.0	0.0	0.0
4	0.0	1.1044610285403774E-5	0.0	1.1044610285403774E-5	0.0
5	0.0	1.1044610285403774E-5	0.0	1.1044610285403774E-5	0.0
6	3.313383085621132E-5	1.1044610285403774E-5	0.0	1.1044610285403774E-5	0.0
7	3.313383085621132E-5	2.2089220570807548E-5	0.0	1.1044610285403774E-5	0.0
8	3.313383085621132E-5	2.2089220570807548E-5	0.0	1.1044610285403774E-5	0.0
9	3.313383085621132E-5	2.2089220570807548E-5	0.0	2.2089220570807548E-5	5.522305142701887E-5
10-11	3.313383085621132E-5	2.2089220570807548E-5	0.0	3.313383085621132E-5	5.522305142701887E-5
12-13	3.313383085621132E-5	3.865613599891321E-5	0.0	3.313383085621132E-5	6.626766171242264E-5
14-15	3.313383085621132E-5	4.4178441141615095E-5	0.0	4.4178441141615095E-5	7.731227199782642E-5
16-17	5.522305142701887E-5	4.4178441141615095E-5	0.0	6.074535656972076E-5	9.940149256863397E-5
18-19	5.522305142701887E-5	4.4178441141615095E-5	0.0	7.178996685512453E-5	9.940149256863397E-5
20-21	5.522305142701887E-5	5.522305142701887E-5	0.0	1.4357993371024905E-4	1.0492379771133586E-4
22-23	5.522305142701887E-5	5.522305142701887E-5	0.0	4.804405474150642E-4	1.3253532342484529E-4
24-25	6.074535656972076E-5	6.074535656972076E-5	0.0	0.0011652063851100981	1.5462454399565285E-4
26-27	6.626766171242264E-5	6.626766171242264E-5	0.0	0.0024905596193585513	1.7119145942375848E-4
28-29	6.626766171242264E-5	6.626766171242264E-5	0.0	0.006869747597521148	1.7671376456646038E-4
30-31	6.626766171242264E-5	6.626766171242264E-5	0.0	0.02086326882912773	1.8223606970916228E-4
32-33	6.626766171242264E-5	6.626766171242264E-5	0.0	0.053406213035069955	2.098475954226717E-4
34-35	6.626766171242264E-5	6.626766171242264E-5	0.0	0.10734808966898199	2.098475954226717E-4
36-37	6.626766171242264E-5	7.178996685512453E-5	0.0	0.18949237866667257	2.098475954226717E-4
38-39	6.626766171242264E-5	1.0492379771133586E-4	0.0	0.3003581767115556	2.098475954226717E-4
40-41	6.626766171242264E-5	1.1044610285403774E-4	0.0	0.41991608305105155	2.3193681599347927E-4
42-43	6.626766171242264E-5	1.1044610285403774E-4	0.0	0.5428757293584516	2.4298142627888304E-4
44-45	6.626766171242264E-5	1.2149071313944152E-4	0.0	0.6958049256752951	2.5402603656428683E-4
46-47	6.626766171242264E-5	1.2701301828214342E-4	0.0	0.877676523245039	2.5954834170698873E-4
48-49	6.626766171242264E-5	1.3253532342484529E-4	0.0	1.0669148758751472	2.6507064684969057E-4
50-51	7.731227199782642E-5	1.4910223885295095E-4	0.0	1.2633874482421952	2.6507064684969057E-4
52-53	7.731227199782642E-5	1.6566915428105661E-4	0.0	1.4619087958171852	2.816375622777962E-4
54-55	7.731227199782642E-5	1.8223606970916228E-4	0.0	1.689665226817639	2.982044777059019E-4
56-57	7.731227199782642E-5	1.8775837485186418E-4	0.0	1.9507432470491561	3.037267828486038E-4
58-59	7.731227199782642E-5	1.8775837485186418E-4	0.0	2.240675311651291	3.092490879913057E-4
60-61	8.835688228323019E-5	1.8775837485186418E-4	0.0	2.541685120369685	3.2581600341941133E-4
62-63	9.940149256863397E-5	1.8775837485186418E-4	0.0	2.848631407116484	3.42382918847517E-4
64-65	9.940149256863397E-5	1.9880298513726794E-4	0.0	3.1646453189076	3.42382918847517E-4
66-67	9.940149256863397E-5	2.098475954226717E-4	0.0	3.5229269542609556	3.4790522399021887E-4
68-69	9.940149256863397E-5	2.098475954226717E-4	0.0	3.9207869505720554	3.5342752913292076E-4
70-71	9.940149256863397E-5	2.3193681599347927E-4	0.0	4.3389855967237265	3.5894983427562266E-4
72-73	1.1044610285403774E-4	2.3193681599347927E-4	0.0	4.75585888964115	3.976059702745359E-4
74-75	1.1044610285403774E-4	2.3193681599347927E-4	0.0	5.176492872360752	4.086505805599396E-4
76-77	1.1044610285403774E-4	2.3193681599347927E-4	0.0	5.631983645141089	4.141728857026415E-4
78-79	1.1044610285403774E-4	2.3745912113618114E-4	0.0	6.137953809230865	4.196951908453434E-4
80-81	1.1044610285403774E-4	2.5402603656428683E-4	0.0	6.671668034357573	4.362621062734491E-4
82-83	1.1044610285403774E-4	2.5402603656428683E-4	0.0	7.211970369519527	4.859628525577661E-4
84-85	1.1044610285403774E-4	2.5402603656428683E-4	0.0	7.749163646886138	4.859628525577661E-4
86-87	1.1044610285403774E-4	2.5402603656428683E-4	0.0	8.30197400319631	4.859628525577661E-4
88	1.1044610285403774E-4	2.5402603656428683E-4	0.0	8.739180423649161	5.080520731285737E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14305	0.0	46.825626	1
GTATCAA	24820	0.0	38.2645	1
TCAACGC	29410	0.0	31.63785	4
ATCAACG	29860	0.0	31.19339	3
CAACGCA	29865	0.0	31.18731	5
TATCAAC	31090	0.0	30.161179	2
AACGCAG	31050	0.0	30.05711	6
ACGCAGA	35630	0.0	26.180136	7
CGCAGAG	37040	0.0	25.225775	8
GTACATG	30080	0.0	25.192959	1
TACATGG	30780	0.0	24.03278	2
ACATGGG	31455	0.0	22.813826	3
GCAGAGT	42100	0.0	21.981773	9
CATGGGG	20825	0.0	20.738018	4
GAGTACT	22940	0.0	20.734608	12-13
AGAGTAC	37000	0.0	19.524382	10-11
CAGAGTA	40865	0.0	19.126957	10-11
GTACTTT	24955	0.0	18.851852	14-15
ATGGGGG	12230	0.0	17.56008	5
AGTACTT	24860	0.0	17.526182	12-13
>>END_MODULE