FastQCFastQC Report
Mon 6 Jun 2016
ERR523077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062601
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT332180.5479166450175428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205090.3382871477110237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149440.24649486251857905No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT96740.15956847564271506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95440.15742418146930665No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC69270.1142578903015389No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC66080.10899612229140596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG225200.028.0619411
TACATGG231350.027.2120912
ACATGGG234900.026.1812253
CATGGGG168100.025.1720434
ATGGGGG101500.023.810165
GAGTACT171450.020.74598712-13
AGAGTAC263700.019.78489910-11
GAGTACA171200.019.6650981
TATAGCG2451.958415E-719.1910695
AGTACAT166950.018.7840942
TATCACG5050.018.620422
GTACTTT189750.018.43542714-15
AGTACTT179500.018.38897712-13
TACCTGG38700.017.7375132
TATAACG4205.638867E-1116.7916282
TGGGGGG102900.016.4369586
GTATATA24650.015.8324451
ACTTTTT231150.015.30520216-17
CATGGGA79450.015.0308474
TACTTTT217300.014.97374314-15
Produced by FastQC (version 0.11.5)