FastQCFastQC Report
Mon 6 Jun 2016
ERR523077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062601
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT328310.5415332462090117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184370.30411039750100655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143450.2366146147503357No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT94260.15547782214267442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88550.14605942235024208No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC70380.1160887876342184No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC65020.10724769781154987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG220950.029.0999031
TACATGG227850.027.848432
ACATGGG230550.026.6612073
CATGGGG167150.024.8813574
ATGGGGG97800.024.1690255
GAGTACT181050.019.94181412-13
TATAACG4850.019.3822542
AGAGTAC272250.019.09805910-11
TATCACG5100.018.4321422
GTACTTT197050.018.18829314-15
AGTACTT189900.017.99746912-13
GAGTACA165050.017.9817031
TACCTGG42150.017.841792
TGGGGGG96550.017.2783586
AGTACAT162850.016.8266162
CATGGGA75150.016.696274
TATACTG24400.016.3703945
TAACGCA5800.016.2046324
GTATAGG17550.015.5441831
ACTTTTT235600.015.29923716-17
Produced by FastQC (version 0.11.5)