FastQCFastQC Report
Mon 6 Jun 2016
ERR523076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11808301
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT546570.46286929847062674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288040.24393009629412396No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT181680.15385786659740466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151450.12825723192523633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124780.10567142555055126No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC120210.10180126675293931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG341000.028.5315741
TACATGG350550.027.2418822
ACATGGG354750.026.1655333
CATGGGG240150.023.8080834
ATGGGGG142500.019.7643975
AGAGTAC401550.019.19831310-11
TACCTGG78150.019.1326582
GAGTACA263100.018.4807381
AGTACAT250850.017.7693422
GAGTACT258300.017.65177312-13
TATCACG10750.017.4955922
AGTACTT278300.017.15132712-13
CATGGGA138450.016.3012814
GTACTTT285600.015.80836714-15
GTATCAA888150.014.5707441
TCAACGC926100.013.7437064
GTATAGG29800.013.7284851
CAACGCA926450.013.71307855
ATGGGGA111400.013.6751285
AACGCAG944600.013.5343236
Produced by FastQC (version 0.11.5)