FastQCFastQC Report
Mon 6 Jun 2016
ERR523076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11808301
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT541080.4582200267422045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257680.21821936957738458No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT177980.1507244776365372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144870.12268488074618017No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC123670.10473140886229104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119440.10114918310432636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG347150.027.3500921
TACATGG356750.025.862672
ACATGGG364450.024.6163883
CATGGGG242950.023.5608224
ATGGGGG146400.020.8975055
AGAGTAC418700.019.07717710-11
TACCTGG83350.018.8910562
GAGTACT263900.018.23359112-13
GAGTACA273650.018.0955831
TATCACG9550.017.718062
AGTACTT282650.017.53135112-13
AGTACAT263950.017.237362
GTACTTT290600.016.22363514-15
GTATCAA911700.014.5715631
TGGGGGG145000.014.4226986
GTATTAG34800.013.9186671
TCAACGC949300.013.8517374
CAACGCA952600.013.7986445
ATTATAC48750.013.6889483
CATGGGA144550.013.6874964
Produced by FastQC (version 0.11.5)