FastQCFastQC Report
Mon 6 Jun 2016
ERR523075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38805
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3840.9895632006184769No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1740.4483958252802474No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1480.38139415023837137No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1280.329854400206159No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1050.27058368766911484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.23192887514495555No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT810.20873598763046No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC790.20358201262723877No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC740.19069707511918568No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC640.1649272001030795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.1623502126014689No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA530.13658033758536273No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA500.12884937508053088No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG470.121118412575699No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC440.11338745007086716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATT500.001679602137.6098334
ACATGGG1501.112172E-625.0732213
CATGGGG950.001279973224.7433094
GTACATG1551.4799189E-624.2644061
AGAGTAC1850.022.87084410-11
TACATGG1652.5492536E-622.7938372
AGTACAT1101.4482521E-619.232312-13
AGTACTT1254.90994E-616.92442512-13
TACTTTT1351.02021695E-515.67076314-15
GAGTACT1250.001596650913.16344212-13
GTACTTT1250.001596650913.16344212-13
GTATCAA5101.9344589E-711.9835251
TCAACGC5454.5772322E-711.2139414
CAACGCA5505.1512325E-711.1119965
ATCAACG5505.1512325E-711.1119963
TATCAAC5555.7905345E-711.0118882
AACGCAG5555.8888327E-710.9976616
GCAGAGT5801.022172E-610.5372379
CGCAGAG5901.2735854E-610.3586418
ACGCAGA5951.4194557E-610.2715927
Produced by FastQC (version 0.11.5)