##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523075_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38805 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.422857879139286 34.0 31.0 34.0 28.0 34.0 2 31.690426491431516 34.0 31.0 34.0 28.0 35.0 3 31.567066099729416 34.0 31.0 34.0 27.0 35.0 4 34.10318257956449 35.0 35.0 37.0 30.0 37.0 5 34.12024223682515 37.0 34.0 37.0 31.0 37.0 6 34.3048060816905 37.0 34.0 37.0 32.0 37.0 7 34.16325215822703 37.0 35.0 37.0 30.0 37.0 8 34.28674139930421 37.0 35.0 37.0 31.0 37.0 9 35.53073057595671 39.0 35.0 39.0 30.0 39.0 10-11 35.61885066357428 38.0 35.0 39.0 30.0 39.0 12-13 35.658562040974104 38.0 35.0 39.0 30.0 39.0 14-15 36.73953098827471 39.0 36.5 41.0 30.0 41.0 16-17 36.63806210539879 39.0 36.5 41.0 30.5 41.0 18-19 36.64404071640253 39.0 36.0 41.0 31.0 41.0 20-21 36.67178198685736 39.0 36.0 41.0 30.5 41.0 22-23 36.59233346218271 39.0 36.0 41.0 30.0 41.0 24-25 36.56929519391831 39.0 36.5 41.0 30.0 41.0 26-27 36.37081561654426 39.0 36.5 41.0 30.0 41.0 28-29 36.218064682386284 39.0 35.0 41.0 28.5 41.0 30-31 36.11588712794743 38.5 36.0 41.0 28.0 41.0 32-33 35.96729802860456 38.0 35.0 41.0 27.5 41.0 34-35 35.8696044324185 38.5 35.0 41.0 27.5 41.0 36-37 35.77629171498518 38.0 35.5 41.0 27.0 41.0 38-39 35.548241206030156 38.0 35.0 40.5 27.0 41.0 40-41 35.491186702744486 38.0 35.0 40.0 25.5 41.0 42-43 35.456809689473005 38.0 35.0 40.0 26.0 41.0 44-45 35.34007215565005 38.0 35.0 40.0 24.5 41.0 46-47 35.1195206803247 38.0 34.5 40.0 24.0 41.0 48-49 35.265403942790876 38.0 35.0 40.0 24.0 41.0 50-51 34.26680840097926 37.5 33.5 39.0 23.0 40.0 52-53 34.444504574152816 37.0 34.5 39.5 23.5 40.0 54-55 35.021878623888675 37.0 34.5 40.0 24.5 41.0 56-57 35.13592320577246 37.5 35.0 40.0 24.5 41.0 58-59 35.08754026542971 38.0 35.0 40.0 24.0 41.0 60-61 34.822187862388866 37.0 35.0 40.0 23.0 41.0 62-63 34.60715114031697 37.0 34.0 40.0 23.0 41.0 64-65 34.3900785981188 36.5 34.0 39.5 23.0 41.0 66-67 34.12142765107589 37.0 34.0 39.0 22.0 41.0 68-69 33.80974101275609 36.0 34.0 39.0 22.0 41.0 70-71 33.39521968818451 36.0 33.0 38.0 21.0 40.5 72-73 33.13462182708414 35.5 33.5 37.5 20.5 40.0 74-75 32.875866512047416 35.0 33.0 37.0 20.0 39.0 76-77 32.56198943435125 35.0 33.0 37.0 20.0 39.0 78-79 32.2670403298544 35.0 32.5 37.0 20.0 38.5 80-81 32.02762530601727 35.0 32.0 36.0 19.0 38.0 82-83 31.83540780827213 35.0 32.0 36.0 19.0 37.5 84-85 31.514328050508958 35.0 32.0 35.5 18.5 37.0 86-87 31.45141090065713 35.0 32.0 36.0 18.5 37.0 88-89 31.16419275866512 35.0 31.0 35.0 18.5 37.0 90-91 31.092075763432547 35.0 31.0 35.0 18.0 36.5 92-93 30.90412318000258 35.0 31.0 35.0 18.0 36.0 94-95 30.835717046772324 34.5 31.0 35.0 18.0 36.0 96-97 30.54422110552764 34.5 30.0 35.0 17.0 36.0 98-99 30.4931194433707 34.5 30.0 35.0 17.0 36.5 100 29.368844221105526 34.0 27.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20579563998191475 1101 2 0.30762082911016364 1101 3 0.4694041668358615 1101 4 0.27783556459744574 1101 5 -0.10526455952540203 1101 6 0.4242522737564087 1101 7 -0.38243201503644997 1101 8 0.5336546245487597 1101 9 -1.091781366998049 1101 10-11 0.01717018823837435 1101 12-13 -0.3192146125913027 1101 14-15 -0.45442935943727036 1101 16-17 -0.8826787037003498 1101 18-19 -0.02563884366257696 1101 20-21 -0.8686647397893168 1101 22-23 -1.211887548128523 1101 24-25 0.06117894960335235 1101 26-27 -0.14865306090157304 1101 28-29 0.4265683999093426 1101 30-31 -0.4267491839289832 1101 32-33 -0.7905413492340259 1101 34-35 0.0239809821452468 1101 36-37 0.016405311312944093 1101 38-39 -0.7635476269091725 1101 40-41 -0.2932988198737334 1101 42-43 -0.2902599581354366 1101 44-45 -0.19548988800475797 1101 46-47 -0.2129903895748626 1101 48-49 -0.1959172051380378 1101 50-51 -0.7190114408820065 1101 52-53 -1.3438599746405586 1101 54-55 -1.376990956604331 1101 56-57 -1.6000617320256083 1101 58-59 -2.492629631033388 1101 60-61 -1.9565073078894741 1101 62-63 -1.4294789133288859 1101 64-65 -0.4633992823696218 1101 66-67 -1.6240929260326666 1101 68-69 -1.6342420549857124 1101 70-71 -0.5074344230969459 1101 72-73 -1.1653987772418972 1101 74-75 -1.5913923598680242 1101 76-77 -0.689780899187646 1101 78-79 -1.8060560403256218 1101 80-81 -2.0007305815906733 1101 82-83 -1.1503169710570482 1101 84-85 -1.7068348130425477 1101 86-87 -1.9676911754586932 1101 88-89 -0.9640533200798345 1101 90-91 -1.417996213768994 1101 92-93 -0.8562303965923768 1101 94-95 -0.9656174311501431 1101 96-97 -1.2928938745564587 1101 98-99 -0.18926136687216655 1101 100 -0.35830911781212293 1102 1 0.9915099256961994 1102 2 0.3790494005387366 1102 3 0.4694041668358615 1102 4 0.992121278883161 1102 5 0.847116392855547 1102 6 0.13853798804212403 1102 7 0.8080441754397398 1102 8 0.08127367216780357 1102 9 -0.23463850985519485 1102 10-11 0.8505035215717172 1102 12-13 -0.08111937449606188 1102 14-15 1.307475402467503 1102 16-17 1.760178439156796 1102 18-19 0.7362659182421822 1102 20-21 0.7980019268773475 1102 22-23 1.0500172137762362 1102 24-25 1.2159408543652503 1102 26-27 0.7680136057650913 1102 28-29 0.8789493522902916 1102 30-31 1.1089651017853086 1102 32-33 0.9713634126707404 1102 34-35 0.9763619345262029 1102 36-37 0.8616434065510461 1102 38-39 0.7959761826146377 1102 40-41 0.5638440372691207 1102 42-43 1.293073375197899 1102 44-45 0.256891064376191 1102 46-47 0.6560572294727578 1102 48-49 -0.6959172051380378 1102 50-51 0.3286076067370445 1102 52-53 0.2989971682165873 1102 54-55 0.5515804719670996 1102 56-57 0.8523192203553549 1102 58-59 0.2454656070618526 1102 60-61 0.7339688825867157 1102 62-63 1.0467115628615886 1102 64-65 0.7389816700113272 1102 66-67 0.9592404073006691 1102 68-69 0.3895674688238131 1102 70-71 1.0997084340459082 1102 72-73 0.7988869370438181 1102 74-75 0.7419409734653044 1102 76-77 0.48879052938378464 1102 78-79 0.45584872157914447 1102 80-81 0.6778408469807573 1102 82-83 -0.04317411391419057 1102 84-85 0.281260425052686 1102 86-87 -0.6224530802205983 1102 88-89 0.07166096563445379 1102 90-91 0.21295616718338906 1102 92-93 0.09615055578857579 1102 94-95 -0.560855526388238 1102 96-97 -0.7690843507469332 1102 98-99 -0.3797375573483599 1102 100 -1.9773567368597398 1103 1 -0.5124583282720536 1103 2 -0.1447601232707889 1103 3 -0.40757996014826503 1103 4 -0.7499422131803328 1103 5 -0.20843916270001017 1103 6 0.43218878169291486 1103 7 0.3001076675032337 1103 8 -0.7719009310067975 1103 9 -0.7227337479504357 1103 10-11 -0.7546552085870175 1103 12-13 -1.5374685808452782 1103 14-15 -0.7699055499134602 1103 16-17 -1.1425993386209896 1103 18-19 0.4366627436390047 1103 20-21 0.2444304983059169 1103 22-23 -0.8329192941602699 1103 24-25 0.14649640992080748 1103 26-27 -0.2776213148698261 1103 28-29 -0.8035903302493921 1103 30-31 -0.6628602950400975 1103 32-33 -0.7945096032022789 1103 34-35 -0.22800314483887973 1103 36-37 -0.46176929186165694 1103 38-39 -0.9738650872266277 1103 40-41 -0.25361628019119564 1103 42-43 -0.7227996406751203 1103 44-45 -0.6617597292745927 1103 46-47 -0.5066411832256534 1103 48-49 -0.24552037974121532 1103 50-51 -1.199170171040734 1103 52-53 -0.26846314924372905 1103 54-55 0.05356459895122612 1103 56-57 -0.6337918907557594 1103 58-59 -0.6751693135730719 1103 60-61 -0.7323009586831191 1103 62-63 -0.9017011355511073 1103 64-65 -2.0387961077664443 1103 66-67 -0.032823084762824806 1103 68-69 -0.5290833248269777 1103 70-71 -0.2475137881763132 1103 72-73 -0.3062717931149166 1103 74-75 0.38082986235420435 1103 76-77 0.9094254500187091 1103 78-79 -1.0243100085795902 1103 80-81 -0.0959686768287682 1103 82-83 -1.0788883996284753 1103 84-85 -0.7881840193917569 1103 86-87 -1.1383260960936141 1103 88-89 -0.7477834788099926 1103 90-91 -1.2949803407531206 1103 92-93 -0.44948436484634513 1103 94-95 -0.2989507644834788 1103 96-97 -0.3722589539215342 1103 98-99 -0.14362644623724918 1103 100 0.30438929488629185 1104 1 1.2827613099708444 1104 2 1.5877980162640917 1104 3 1.956115130291014 1104 4 0.6886882777757393 1104 5 0.8936280207625202 1104 6 0.9192688850520909 1104 7 1.5300818277099566 1104 8 0.18869338423868243 1104 9 1.2210647016619731 1104 10-11 1.6636264451597498 1104 12-13 1.9050378791030411 1104 14-15 1.888040186520655 1104 16-17 1.029558284118032 1104 18-19 1.2393113224503765 1104 20-21 1.5535390254377006 1104 22-23 0.9755432381394726 1104 24-25 1.4775687613419919 1104 26-27 1.2995750675591182 1104 28-29 2.284264966908232 1104 30-31 1.8697624439779972 1104 32-33 1.669314686203407 1104 34-35 1.6773586122670636 1104 36-37 1.5773133954768426 1104 38-39 1.7638610109645825 1104 40-41 1.0286834614108713 1104 42-43 2.440637051831345 1104 44-45 1.2591059038003394 1104 46-47 1.1283717366709851 1104 48-49 1.65015145488411 1104 50-51 1.7123285369696006 1104 52-53 2.3084102357691947 1104 54-55 2.4466524542483867 1104 56-57 1.924024646711942 1104 58-59 2.042530944824861 1104 60-61 2.386239092996462 1104 62-63 2.3941644975238248 1104 64-65 2.2137878715617205 1104 66-67 2.018210506968437 1104 68-69 2.455735796620047 1104 70-71 2.183041767379244 1104 72-73 1.836816062182244 1104 74-75 2.48418903548081 1104 76-77 1.8157561993727072 1104 78-79 1.4414522653222193 1104 80-81 1.18697705960534 1104 82-83 1.8028945461079573 1104 84-85 2.242500735130207 1104 86-87 2.013482689436106 1104 88-89 1.2790253067197206 1104 90-91 1.6636759899962321 1104 92-93 1.354456203629109 1104 94-95 1.3870957471444285 1104 96-97 2.2491880745022392 1104 98-99 1.478789574434586 1104 100 0.805035289718333 1105 1 0.32781944950572495 1105 2 1.073989876729211 1105 3 0.2104755954072921 1105 4 1.03825223126411 1105 5 -0.05913360714445304 1105 6 0.3974665594706934 1105 7 -0.48808677694120917 1105 8 0.700321291215424 1105 9 1.2668495853829 1105 10-11 0.8147892358574254 1105 12-13 1.0736425302658361 1105 14-15 0.7717611167532112 1105 16-17 0.7497617724901247 1105 18-19 1.4140932991945618 1105 20-21 1.0517221649725883 1105 22-23 0.8163862613952872 1105 24-25 1.0128158543652503 1105 26-27 1.2814064629079525 1105 28-29 0.9012707808617222 1105 30-31 1.1201258160710168 1105 32-33 1.2488931745754996 1105 34-35 1.5254690773833417 1105 36-37 1.4010779303605645 1105 38-39 1.024398801662258 1105 40-41 0.8956892753643615 1105 42-43 1.607061470435994 1105 44-45 2.008379159614293 1105 46-47 2.418706038996568 1105 48-49 1.6537851758143418 1105 50-51 0.9848576067370445 1105 52-53 1.3756340729784924 1105 54-55 1.3990507100623404 1105 56-57 1.6544025536886835 1105 58-59 0.613025130871371 1105 60-61 1.2659629302057667 1105 62-63 1.6746877533377784 1105 64-65 1.2650233366779986 1105 66-67 1.7137046930149538 1105 68-69 0.859805564061908 1105 70-71 0.38616676737924394 1105 72-73 1.6329643179961977 1105 74-75 1.1273576401319758 1105 76-77 1.3608143389075948 1105 78-79 1.571176102531524 1105 80-81 1.414447989837896 1105 82-83 2.212778267038189 1105 84-85 2.1614687583860217 1105 86-87 1.3790350150174966 1105 88-89 1.316452632301118 1105 90-91 1.2657835481357687 1105 92-93 0.29704341293143344 1105 94-95 2.013549235516521 1105 96-97 1.2978799349673515 1105 98-99 0.583804109318308 1105 100 0.13425040599740257 1106 1 0.8409008448545592 1106 2 0.6575654581245622 1106 3 1.0026267581979873 1106 4 0.8747347894036466 1106 5 0.847116392855547 1106 6 0.8960130710986007 1106 7 1.3440353160820493 1106 8 0.7468329191223972 1106 9 0.5699019109642975 1106 10-11 0.39618458469463036 1106 12-13 0.3934099721262996 1106 14-15 0.7019936748927478 1106 16-17 0.9132792143505881 1106 18-19 1.1346601596596742 1106 20-21 1.2628413510190981 1106 22-23 0.9987990520929628 1106 24-25 1.3147780636675748 1106 26-27 0.5205053001172502 1106 28-29 0.8075207808617222 1106 30-31 0.8116229090942753 1106 32-33 1.1344309652731681 1106 34-35 1.0610795424996198 1106 36-37 0.7284761861745181 1106 38-39 -0.23613898903541752 1106 40-41 0.08682299629459322 1106 42-43 -0.3384327156105158 1106 44-45 -0.25252200317640927 1106 46-47 4.6475992679972933E-4 1106 48-49 -0.03589505674379723 1106 50-51 -0.22953192814667744 1106 52-53 0.6688753520482535 1106 54-55 0.6210710588995525 1106 56-57 0.8077455769444981 1106 58-59 0.5541588518016098 1106 60-61 1.409494906949952 1106 62-63 0.3709086835703346 1106 64-65 0.5742529878407936 1106 66-67 1.0763500418521588 1106 68-69 1.316200912899113 1106 70-71 1.8225766511001709 1106 72-73 1.7089090854380586 1106 74-75 1.9725611285040685 1106 76-77 1.850639920302946 1106 78-79 1.6623824978803583 1106 80-81 0.907907292163479 1106 82-83 0.4889410577358646 1106 84-85 0.6843612002464852 1106 86-87 0.43208734059889053 1106 88-89 0.6511183299755352 1106 90-91 0.3729783155776296 1106 92-93 -0.13391588939414945 1106 94-95 0.25918877040024313 1106 96-97 0.435234586130143 1106 98-99 0.14158027210900315 1106 100 -0.5903135474909682 1107 1 -2.672180550494275 1107 2 -2.988510123270789 1107 3 -2.352024404592708 1107 4 -3.55549776873589 1107 5 -4.152883607144453 1107 6 -2.8525334405293066 1107 7 -3.144336776941209 1107 8 -3.299678708784576 1107 9 -3.3581504146171 1107 10-11 -3.5758357641425746 1107 12-13 -4.191982469734164 1107 14-15 -2.1032388832467888 1107 16-17 -3.2502382275098753 1107 18-19 -3.617156700805438 1107 20-21 -2.9482778350274117 1107 22-23 -4.027363738604713 1107 24-25 -3.9559341456347497 1107 26-27 -2.7342185370920475 1107 28-29 -3.270604219138278 1107 30-31 -3.754874183928983 1107 32-33 -3.5636068254245004 1107 34-35 -2.9120309226166583 1107 36-37 -2.6457970696394355 1107 38-39 -3.100601198337742 1107 40-41 -3.5261857246356385 1107 42-43 -3.892938529564006 1107 44-45 -3.241620840385707 1107 46-47 -2.503168961003432 1107 48-49 -3.877464824185658 1107 50-51 -3.3276423932629555 1107 52-53 -4.124365927021508 1107 54-55 -4.27282428993766 1107 56-57 -3.7987224463113165 1107 58-59 -4.308849869128629 1107 60-61 -3.7184120697942333 1107 62-63 -3.5128122466622216 1107 64-65 -4.406851663322001 1107 66-67 -3.9269203069850462 1107 68-69 -4.296444435938092 1107 70-71 -3.645083232620756 1107 72-73 -3.5545356820038023 1107 74-75 -4.060142359868024 1107 76-77 -4.170435661092405 1107 78-79 -4.522573897468476 1107 80-81 -3.913677010162104 1107 82-83 -3.709096732961811 1107 84-85 -2.5572812416139783 1107 86-87 -2.3709649849825034 1107 88-89 -1.933547367698882 1107 90-91 -3.1092164518642313 1107 92-93 -3.3123315870685666 1107 94-95 -2.814575764483479 1107 96-97 -3.0302450650326485 1107 98-99 -3.791195890681692 1107 100 -4.146999594002597 1108 1 0.9438908780771555 1108 2 1.3838113053006431 1108 3 1.2265470239787213 1108 4 1.6873593741212503 1108 5 1.3042592499984025 1108 6 0.5956808451849795 1108 7 1.655663223058788 1108 8 1.557464148358278 1108 9 1.7320281568114737 1108 10-11 2.0290749501431335 1108 12-13 1.93792824455155 1108 14-15 2.086046831038928 1108 16-17 2.2149403439186983 1108 18-19 1.9100754420517063 1108 20-21 2.5003828792583036 1108 22-23 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1.8735120770145244 2312 34-35 1.1436093212857799 2312 36-37 1.269599271823978 2312 38-39 1.5247798992232333 2312 40-41 1.7958417143887573 2312 42-43 1.4431895192164816 2312 44-45 1.205787696199664 2312 46-47 0.6850932341185185 2312 48-49 1.1118644441070202 2312 50-51 1.2676319969809455 2312 52-53 1.1793688290760542 2312 54-55 1.612846441769662 2312 56-57 0.9356525536886835 2312 58-59 1.165616594286 2312 60-61 0.7937830521569893 2312 62-63 1.0115779972402166 2312 64-65 0.8385751659462954 2312 66-67 0.9949546930149538 2312 68-69 1.2470006860131306 2312 70-71 -0.09744603749880554 2312 72-73 -0.9482094624916044 2312 74-75 -0.41456309157534577 2312 76-77 -0.7443685879216737 2312 78-79 -0.33393060478555014 2312 80-81 -0.2585703028450297 2312 82-83 0.24021729142843284 2312 84-85 0.8584961974104175 2312 86-87 0.28376062477359554 2312 88-89 1.2810105591303902 2312 90-91 1.1061036700869913 2312 92-93 0.5569519495167974 2312 94-95 0.3595858208823728 2312 96-97 0.6263860325283268 2312 98-99 1.3418071580987956 2312 100 1.5679394303876464 2313 1 0.3584567044076863 2313 2 0.061122229670388606 2313 3 1.2411128503092534 2313 4 0.25822772146018735 2313 5 0.49417521638496 2313 6 0.8472214614314808 2313 7 1.0909573407058488 2313 8 0.5042428598428756 2313 9 0.5762858598927068 2313 10-11 0.7167500201711547 2313 12-13 0.3922699812462298 2313 14-15 0.5756826853806629 2313 16-17 0.2552764783724797 2313 18-19 0.9652575148808324 2313 20-21 0.5802148120314143 2313 22-23 0.5535186143364612 2313 24-25 0.9953526190711344 2313 26-27 1.1153525413393197 2313 28-29 0.4839913690970121 2313 30-31 0.6565841494043454 2313 32-33 1.1303269981049127 2313 34-35 0.2858857440500131 2313 36-37 0.20408038134095108 2313 38-39 0.8758081153877413 2313 40-41 0.4637039812467094 2313 42-43 1.0696840194556074 2313 44-45 0.6658546498103703 2313 46-47 0.8479339801730319 2313 48-49 0.2732704699319939 2313 50-51 0.12272525379587051 2313 52-53 0.7527786808216277 2313 54-55 1.0004600237878307 2313 56-57 1.5140839262377028 2313 58-59 0.9506476798909773 2313 60-61 1.2619800870685154 2313 62-63 0.8107171651024885 2313 64-65 0.5183934347172112 2313 66-67 0.9851507714463281 2313 68-69 0.5779428189638693 2313 70-71 1.0457868654184566 2313 72-73 1.067094219956985 2313 74-75 1.1880194048378598 2313 76-77 1.5090986526330852 2313 78-79 1.6995462005707367 2313 80-81 0.8746195584653478 2313 82-83 1.367190031744073 2313 84-85 1.457731013287983 2313 86-87 1.3502359954096512 2313 88-89 0.45891586759523406 2313 90-91 0.6037124697043943 2313 92-93 1.3405483148922208 2313 94-95 2.1030100198302506 2313 96-97 1.918590719281081 2313 98-99 0.3800663642202693 2313 100 -0.9423427312575008 2314 1 -0.7119532777670017 2314 2 -0.9174873959980623 2314 3 0.44343014086183885 2314 4 -0.55549776873589 2314 5 -0.6074290616899063 2314 6 -0.24457889507475983 2314 7 -0.48524586785030266 2314 8 -0.45876961787548254 2314 9 0.3833268581101734 2314 10-11 -0.07157440050621489 2314 12-13 0.0054607120840231005 2314 14-15 -0.5464207014286089 2314 16-17 -1.4064882275098753 2314 18-19 -0.25778170080543816 2314 20-21 -0.19117556230014543 2314 22-23 0.1771817159407405 2314 24-25 -0.14769550927110942 2314 26-27 -0.8862071734556878 2314 28-29 0.04615714449808195 2314 30-31 0.08461445243465704 2314 32-33 0.05144999275731266 2314 34-35 -0.5739627407984784 2314 36-37 -1.1600016150939823 2314 38-39 0.0854783471168048 2314 40-41 -0.5048789064538184 2314 42-43 -0.24805216592764623 2314 44-45 1.0225837050688398 2314 46-47 0.4428537662692946 2314 48-49 0.6850351758143418 2314 50-51 -0.12593784780840878 2314 52-53 0.09012270934212552 2314 54-55 -0.3353242899376596 2314 56-57 0.16292528096141012 2314 58-59 -0.2648157782195355 2314 60-61 0.14522429384212643 2314 62-63 0.6349150260650518 2314 64-65 0.428375609405272 2314 66-67 -0.368681670621406 2314 68-69 0.17798738224372812 2314 70-71 -0.1692309598934827 2314 72-73 -0.1269788638219893 2314 74-75 0.36315309467742907 2314 76-77 0.5369507025439546 2314 78-79 0.17771019344061756 2314 80-81 0.5025161716560831 2314 82-83 0.3832328124927358 2314 84-85 0.21686648565874833 2314 86-87 0.9386941059265901 2314 88-89 1.2496912686647583 2314 90-91 0.582544911772132 2314 92-93 1.1237479583859802 2314 94-95 0.28059469006197446 2314 96-97 0.6416299349673515 2314 98-99 0.5596563820455813 2314 100 1.6200458605428558 2315 1 -0.30968055049427434 2315 2 0.08023987672921251 2315 3 -0.5770244045927093 2315 4 -1.1054977687358871 2315 5 -0.3528836071444559 2315 6 0.20996655947069343 2315 7 0.280663223058788 2315 8 -0.8496787087845732 2315 9 -0.8894004146171 2315 10-11 -0.14771076414257323 2315 12-13 -0.18885746973416673 2315 14-15 -0.6657388832467888 2315 16-17 -0.031488227509875344 2315 18-19 -0.9452817008054382 2315 20-21 -0.8514028350274145 2315 22-23 -0.6148637386047113 2315 24-25 -0.884059145634744 2315 26-27 -0.5748435370920504 2315 28-29 -0.5424792191382721 2315 30-31 -0.5392491839289804 2315 32-33 -0.07923182542450036 2315 34-35 -0.2557809226166583 2315 36-37 -0.3645470696394355 2315 38-39 -0.1599761983377448 2315 40-41 0.28943927536435865 2315 42-43 0.27581147043599685 2315 44-45 0.08962915961429019 2315 46-47 -0.159418961003432 2315 48-49 -0.6024648241856596 2315 50-51 -2.6713923932629555 2315 52-53 -0.45874092702150193 2315 54-55 -1.1978242899376568 2315 56-57 -1.0893474463113222 2315 58-59 -1.1307248691286276 2315 60-61 -1.6934120697942276 2315 62-63 -1.1878122466622187 2315 64-65 -1.181851663322007 2315 66-67 -2.055045306985047 2315 68-69 -2.077694435938092 2315 70-71 -2.316958232620756 2315 72-73 -1.970160682003801 2315 74-75 -1.3038923598680228 2315 76-77 -2.0641856610924023 2315 78-79 -0.8381988974684731 2315 80-81 -0.6793020101621039 2315 82-83 -0.912221732961811 2315 84-85 -1.3354062416139776 2315 86-87 -2.002214984982505 2315 88-89 -0.8741723676988826 2315 90-91 -0.6810914518642299 2315 92-93 -2.9842065870685666 2315 94-95 -1.8739507644834816 2315 96-97 -1.1833700650326477 2315 98-99 -0.5130708906816892 2315 100 -1.2594995940025981 2316 1 -0.0301350959488218 2316 2 0.2870580585473945 2316 3 -0.05656985913816115 2316 4 -0.5782250414631633 2316 5 -0.9256108798717264 2316 6 -0.15366980416567344 2316 7 -0.9397913223957559 2316 8 -0.5042241633300293 2316 9 0.8378723126556267 2316 10-11 -0.04884712777893441 2316 12-13 -0.06272110609780412 2316 14-15 -1.5009661559740621 2316 16-17 0.21851177249012466 2316 18-19 0.3558546628309216 2316 20-21 -1.4525391986637715 2316 22-23 0.24536353412256062 2316 24-25 0.1363953998197971 2316 26-27 0.40924737199885897 2316 28-29 0.3643389626799092 2316 30-31 0.4255235433437434 2316 32-33 0.24463181093913988 2316 34-35 0.8805827137469819 2316 36-37 -0.28500161509398225 2316 38-39 -0.15315801651955496 2316 40-41 0.5292120026370881 2316 42-43 -0.6003248932003658 2316 44-45 -1.1365072040220667 2316 46-47 -3.280519125254955E-4 2316 48-49 -0.08769209691293156 2316 50-51 1.0786076067370445 2316 52-53 -0.46669547247605436 2316 54-55 0.5055848009714339 2316 56-57 -0.33707471903858277 2316 58-59 -0.6966339600377225 2316 60-61 0.054315202933032936 2316 62-63 -0.28553951938949496 2316 64-65 0.21246651849618559 2316 66-67 -0.06186348880322612 2316 68-69 -0.5379217086653654 2316 70-71 -0.6237764144389359 2316 72-73 0.6798393179961977 2316 74-75 0.26088036740470244 2316 76-77 -1.7925947520014986 2316 78-79 0.14361928434971105 2316 80-81 -0.6679383737984637 2316 82-83 0.3036873579472825 2316 84-85 -1.2035880597957984 2316 86-87 0.8932395604720433 2316 88-89 1.1587821777556648 2316 90-91 -1.1560914518642313 2316 92-93 0.4646570492950701 2316 94-95 0.22377650824379458 2316 96-97 0.6757208440582616 2316 98-99 0.44602001840921446 2316 100 0.3245913150883126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 98.0 1 9.0 2 7.0 3 5.0 4 7.0 5 7.0 6 6.0 7 19.0 8 45.0 9 102.0 10 145.0 11 173.0 12 220.0 13 217.0 14 204.0 15 180.0 16 203.0 17 198.0 18 198.0 19 245.0 20 267.0 21 287.0 22 322.0 23 350.0 24 333.0 25 351.0 26 407.0 27 390.0 28 478.0 29 538.0 30 700.0 31 840.0 32 1141.0 33 1436.0 34 2313.0 35 4416.0 36 6083.0 37 6375.0 38 7574.0 39 1912.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.0420905851366 18.95367453390487 13.223674017455972 22.780560863502554 2 15.911379657603222 21.5870064812663 38.78689286544271 23.714720995687763 3 21.706389255492585 24.767745775431514 29.032425018761483 24.493439950314418 4 13.430441644459393 15.950428190732453 38.09474528472743 32.52438488008072 5 13.972985561248253 37.869378460901515 34.26227811416447 13.895357863685764 6 32.128171308868026 36.25572193343161 17.50846975457108 14.107637003129284 7 28.047077082255562 32.24004138644594 21.541645111226075 18.171236420072425 8 25.631012723699182 33.117823523326784 20.510499637943518 20.740664115030516 9 26.129015570844754 16.300243132791888 21.06202472712224 36.508716569241116 10-11 25.85578364961994 25.00387817363876 28.145198821035212 20.995139355706087 12-13 26.847242872842457 23.007304932445535 27.700562415152884 22.44488977955912 14-15 23.39022498060512 25.03361779156969 26.41711921386087 25.159038013964313 16-17 23.17388493859082 27.508726567550095 26.586942469295412 22.730446024563673 18-19 22.77677185721676 28.07294361096741 27.35773409208484 21.792550439730988 20-21 23.66004449273113 26.02954110404056 27.315174090744478 22.995240312483833 22-23 23.20296940068803 26.212462171180263 27.077675176534495 23.506893251597216 24-25 24.039468749595873 26.137054327725117 27.36948284557787 22.453994077101143 26-27 23.509257343814646 25.4693318163012 28.41590815059992 22.605502689284236 28-29 22.518200509484952 27.58977409385385 27.580722330699704 22.31130306596149 30-31 23.697852701252696 25.992527762336305 27.018990860082997 23.290628676328005 32-33 23.2375067862775 26.360487060830902 27.565212895219876 22.836793257671726 34-35 24.356819650937297 26.14091790562379 27.344537815126053 22.157724628312863 36-37 24.18439257774617 25.909355401823237 27.560612917825043 22.345639102605546 38-39 23.294102431453698 26.760217278841182 27.752198654940507 22.193481634764613 40-41 23.59889256882633 26.7711136410681 27.262730283585178 22.367263506520388 42-43 23.03126697183645 26.78640701373264 26.58597770708873 23.596348307342176 44-45 23.58584487283917 25.865301320093607 27.127212546707526 23.421641260359696 46-47 23.875463842881707 26.797512379918025 26.74062293937396 22.586400837826307 48-49 23.774360831274972 26.367245593389114 27.090149624322677 22.76824395101323 50-51 22.564334669597827 26.887365834734254 27.73955773955774 22.808741756110177 52-53 23.25424956663476 27.41973040801014 26.159737134873613 23.166282890481487 54-55 22.965071151358345 26.56791720569211 26.897800776196632 23.56921086675291 56-57 23.540674808528255 26.326071206789486 27.19286897122749 22.940385013454772 58-59 24.388792590484567 26.27221690425064 26.40286653041161 22.93612397485318 60-61 23.646734701796593 26.39530221308189 27.66546376417938 22.292499320942145 62-63 22.75720217844068 26.662613352651256 27.594013168958515 22.986171299949547 64-65 22.53107578481717 27.305299375250613 27.095756101977724 23.067868737954495 66-67 23.11998965606413 26.274890095681407 26.480475821049907 24.12464442720455 68-69 22.515323143766004 26.754079706209428 26.902785320816196 23.827811829208372 70-71 23.551928821756952 26.64011276785599 26.7164121199581 23.09154629042896 72-73 23.119996896297543 26.07368706919963 27.625538298395124 23.1807777361077 74-75 22.49809261486338 27.61117792347183 26.92193298935744 22.968796472307353 76-77 22.853522292334745 26.265904623978482 26.740457225612914 24.14011585807386 78-79 23.21264568538425 26.599143673923447 26.834568667779113 23.353641972913188 80-81 23.556659679631498 26.665890329425768 26.577905442124063 23.199544548818672 82-83 23.711006740933378 26.281877110584944 27.284607123911552 22.72250902457012 84-85 22.84535175554348 27.1016067686098 27.075732877952856 22.977308597893863 86-87 23.043292675039776 26.88491935170933 26.945712770498375 23.12607520275252 88-89 22.481863207852165 27.120485199601703 26.234659700759096 24.16299189178704 90-91 23.052548532702183 26.327293420763333 26.41265406950426 24.207503977030225 92-93 23.66855487442125 26.93023977651897 26.427148806290578 22.9740565427692 94-95 23.625755203953585 26.486150820859798 26.47450741943413 23.413586555752484 96-97 22.28921118341894 26.650537564850634 28.01288603107655 23.047365220653877 98-99 22.948338098873087 27.157107554566508 26.093594338280006 23.800960008280395 100 22.913646717746946 25.893042037688964 26.40298198384759 24.790329260716504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 47.5 2 26.0 3 14.0 4 6.0 5 6.0 6 13.5 7 18.0 8 26.0 9 30.0 10 25.5 11 22.5 12 21.5 13 18.0 14 20.5 15 25.5 16 24.0 17 33.0 18 38.5 19 39.5 20 43.5 21 44.5 22 51.0 23 66.0 24 78.0 25 100.5 26 126.5 27 143.5 28 181.0 29 226.5 30 265.5 31 317.0 32 413.0 33 495.0 34 564.5 35 655.0 36 788.5 37 884.0 38 961.5 39 1087.0 40 1120.0 41 1126.0 42 1196.0 43 1275.0 44 1366.5 45 1467.5 46 1541.0 47 1761.0 48 2264.0 49 2227.5 50 1728.5 51 1543.0 52 1437.5 53 1326.0 54 1291.5 55 1242.0 56 1098.0 57 993.5 58 877.5 59 757.0 60 698.0 61 574.5 62 437.0 63 337.0 64 254.0 65 196.0 66 158.5 67 125.5 68 89.5 69 71.0 70 55.5 71 44.5 72 36.0 73 27.5 74 22.5 75 17.5 76 12.5 77 6.0 78 4.5 79 3.5 80 3.0 81 3.5 82 1.5 83 1.5 84 2.0 85 0.5 86 0.0 87 1.5 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20358201262723877 2 0.20100502512562815 3 0.41747197526092 4 0.396856075248035 5 0.4097410127560881 6 0.35562427522226514 7 0.3736631877335395 8 0.3530472877206545 9 0.3685092127303182 10-11 0.3272774127045484 12-13 0.34145084396340675 14-15 0.3478933127174333 16-17 0.33500837520938026 18-19 0.3736631877335395 20-21 0.37881716273676075 22-23 0.3710862002319289 24-25 0.36464373147790236 26-27 0.3427393377142121 28-29 0.3569127689730705 30-31 0.3311428939569643 32-33 0.31954645019971656 34-35 0.33500837520938026 36-37 0.3543357814714599 38-39 0.3736631877335395 40-41 0.4045870377528669 42-43 0.35562427522226514 44-45 0.3440278314650174 46-47 0.3440278314650174 48-49 0.36464373147790236 50-51 0.3607782502254864 52-53 0.396856075248035 54-55 0.3994330627496457 56-57 0.4045870377528669 58-59 0.3917021002448138 60-61 0.3826826439891766 62-63 0.39556758149722976 64-65 0.38525963149078724 66-67 0.3478933127174333 68-69 0.35562427522226514 70-71 0.36464373147790236 72-73 0.36464373147790236 74-75 0.3594897564746811 76-77 0.3530472877206545 78-79 0.39041360649400847 80-81 0.41747197526092 82-83 0.413606494008504 84-85 0.4020100502512563 86-87 0.38525963149078724 88-89 0.36206674397629174 90-91 0.3749516814843448 92-93 0.3710862002319289 94-95 0.40329854400206155 96-97 0.4084525190052828 98-99 0.41102950650689346 100 0.44581883777863673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 38805.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.04252029377658 #Duplication Level Percentage of deduplicated Percentage of total 1 95.5496408231018 86.03530472877206 2 3.723419478549555 6.705321479190826 3 0.40926132623565437 1.105527638190955 4 0.1144786926533299 0.41231800025769877 5 0.0543773790103317 0.2448138126530086 6 0.02289573853066598 0.12369540007730961 7 0.02575770584699923 0.1623502126014689 8 0.005723934632666495 0.04123180002576988 9 0.0 0.0 >10 0.0601013136429982 1.4508439634067776 >50 0.020033771214332734 1.2988017008117512 >100 0.014309836581666237 2.4197912640123698 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 384 0.9895632006184769 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 174 0.4483958252802474 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 148 0.38139415023837137 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 128 0.329854400206159 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 105 0.27058368766911484 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.23192887514495555 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 81 0.20873598763046 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 79 0.20358201262723877 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 74 0.19069707511918568 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 64 0.1649272001030795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.1623502126014689 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 53 0.13658033758536273 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 50 0.12884937508053088 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 47 0.121118412575699 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 44 0.11338745007086716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0025769875016106174 0.0 5 0.0 0.0 0.0 0.0025769875016106174 0.0 6 0.0 0.0 0.0 0.0025769875016106174 0.0 7 0.0 0.0 0.0 0.0025769875016106174 0.0 8 0.0 0.0 0.0 0.0025769875016106174 0.0 9 0.0 0.0 0.0 0.0025769875016106174 0.0 10-11 0.0 0.0 0.0 0.0025769875016106174 0.0 12-13 0.0 0.0 0.0 0.0025769875016106174 0.0 14-15 0.0 0.0 0.0 0.0025769875016106174 0.0 16-17 0.0 0.0 0.0 0.0025769875016106174 0.0 18-19 0.0 0.0 0.0 0.0025769875016106174 0.0 20-21 0.0 0.0 0.0 0.0025769875016106174 0.0 22-23 0.0 0.0 0.0 0.0025769875016106174 0.0 24-25 0.0 0.0 0.0 0.0025769875016106174 0.0 26-27 0.0 0.0 0.0 0.0025769875016106174 0.0 28-29 0.0 0.0 0.0 0.0025769875016106174 0.0 30-31 0.0 0.0 0.0 0.0038654812524159263 0.0 32-33 0.0 0.0 0.0 0.015461925009663705 0.0 34-35 0.0 0.0 0.0 0.03221234377013271 0.0 36-37 0.0 0.0 0.0 0.051539750032212346 0.0 38-39 0.0 0.0 0.0 0.06829016879268135 0.0 40-41 0.0 0.0 0.0 0.11209895632006184 0.0 42-43 0.0 0.0 0.0 0.1533307563458317 0.0 44-45 0.0 0.0 0.0 0.1932740626207963 0.0 46-47 0.0 0.0 0.0 0.26414121891508824 0.0 48-49 0.0 0.0 0.0 0.3324313877077696 0.0 50-51 0.0 0.0 0.0 0.4020100502512563 0.0 52-53 0.0 0.0 0.0 0.45483829403427395 0.0 54-55 0.0 0.0 0.0 0.5373018940858136 0.0 56-57 0.0 0.0 0.0 0.6378044066486277 0.0 58-59 0.0 0.0 0.0 0.7447493879654684 0.0 60-61 0.0 0.0 0.0 0.8645793067903621 0.0 62-63 0.0 0.0 0.0 1.015333075634583 0.0 64-65 0.0 0.0 0.0 1.1879912382424944 0.0 66-67 0.0 0.0 0.0 1.3374565133359104 0.0 68-69 0.0 0.0 0.0 1.5590774384744235 0.0 70-71 0.0 0.0 0.0 1.7523515010952198 0.0 72-73 0.0 0.0 0.0 1.9649529699780954 0.0 74-75 0.0 0.0 0.0 2.2123437701327147 0.0 76-77 0.0 0.0 0.0 2.44427264527767 0.0 78-79 0.0 0.0 0.0 2.709702357943564 0.0 80-81 0.0 0.0 0.0 3.056307176910192 0.0 82-83 0.0 0.0 0.0 3.3913155521195724 0.0 84-85 0.0 0.0 0.0 3.71601597732251 0.0 86-87 0.0 0.0 0.0 4.047158871279474 0.0 88 0.0 0.0 0.0 4.321608040201005 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCATT 50 0.0016796021 37.609833 4 ACATGGG 150 1.112172E-6 25.073221 3 CATGGGG 95 0.0012799732 24.743309 4 GTACATG 155 1.4799189E-6 24.264406 1 AGAGTAC 185 0.0 22.870844 10-11 TACATGG 165 2.5492536E-6 22.793837 2 AGTACAT 110 1.4482521E-6 19.2323 12-13 AGTACTT 125 4.90994E-6 16.924425 12-13 TACTTTT 135 1.02021695E-5 15.670763 14-15 GAGTACT 125 0.0015966509 13.163442 12-13 GTACTTT 125 0.0015966509 13.163442 12-13 GTATCAA 510 1.9344589E-7 11.983525 1 TCAACGC 545 4.5772322E-7 11.213941 4 CAACGCA 550 5.1512325E-7 11.111996 5 ATCAACG 550 5.1512325E-7 11.111996 3 TATCAAC 555 5.7905345E-7 11.011888 2 AACGCAG 555 5.8888327E-7 10.997661 6 GCAGAGT 580 1.022172E-6 10.537237 9 CGCAGAG 590 1.2735854E-6 10.358641 8 ACGCAGA 595 1.4194557E-6 10.271592 7 >>END_MODULE