FastQCFastQC Report
Mon 6 Jun 2016
ERR523075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38805
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3470.8942146630588841No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2100.5411673753382297No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1530.39427908774642445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1130.2911995876819997No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1060.27316067517072545No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC970.24996778765622985No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT940.242236825151398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.21904393763690247No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC840.21646695013529185No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC690.17781213761113257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.17265816260791134No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG630.1623502126014689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.14688828759180517No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC550.14173431258858393No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA510.1314263625821415No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA510.1314263625821415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA156.819507E-494.110961
ACTGTGA250.001693178337.5958816-17
TGTTGGT350.0087909626.85419844-45
GTACATG1456.39266E-419.4712351
AGAGTAC1504.0017767E-918.82219310-11
ACATCTC1550.001009793118.19155394
TACTTTT650.00794875418.08659614-15
TACATGG1650.001535749517.0890332
ACATGGG1450.004871146816.205123
GAGTACA1250.001585708213.17553512-13
AGTACAT1300.002129985312.66878512-13
AGTGGTA4450.00104576159.5168399
CAGTGGT4550.00128149839.3076788
GCAGTGG4650.00158139259.0957777
TAAGCAG4250.007823458.8460884
GTAAGCA4300.0086106368.7432273
GAGTAAG4350.0093656888.6538821
AGTAAGC4350.00946550358.6427312
CAACGCA5100.00365727848.2932084
TCAACGC5200.00435771758.1337233
Produced by FastQC (version 0.11.5)