##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38805 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44074217240046 34.0 31.0 34.0 31.0 34.0 2 32.68980801443113 34.0 31.0 34.0 31.0 34.0 3 32.80402010050251 34.0 31.0 34.0 30.0 35.0 4 35.648808143280505 37.0 35.0 37.0 33.0 37.0 5 35.7716789073573 37.0 35.0 37.0 34.0 37.0 6 35.90908388094318 37.0 35.0 37.0 34.0 37.0 7 35.861435382038394 37.0 36.0 37.0 35.0 37.0 8 35.863754670789845 37.0 36.0 37.0 35.0 37.0 9 37.19286174462054 39.0 36.0 39.0 35.0 39.0 10-11 37.09413735343384 39.0 36.0 39.0 34.5 39.0 12-13 37.160675170725426 39.0 36.0 39.0 34.5 39.0 14-15 38.27640767942276 40.0 37.0 41.0 34.0 41.0 16-17 38.317523515010954 40.0 37.0 41.0 34.5 41.0 18-19 38.22254864063909 40.0 37.0 41.0 34.0 41.0 20-21 38.205888416441184 40.0 37.0 41.0 34.0 41.0 22-23 38.06066228578791 39.5 36.5 41.0 34.0 41.0 24-25 38.0476742687798 39.0 36.5 41.0 33.5 41.0 26-27 37.8574410514109 39.0 36.5 41.0 34.0 41.0 28-29 37.74947816003092 39.0 37.0 41.0 33.5 41.0 30-31 37.480865867800546 39.0 36.0 41.0 33.0 41.0 32-33 37.38661254992913 38.5 36.5 41.0 32.5 41.0 34-35 37.359927844349954 38.5 37.0 41.0 32.5 41.0 36-37 37.27903620667439 38.0 36.0 41.0 33.0 41.0 38-39 37.25349826053343 38.5 36.0 41.0 33.0 41.0 40-41 37.26616415410385 39.0 36.0 41.0 33.0 41.0 42-43 37.26982347635614 38.5 36.0 41.0 33.0 41.0 44-45 37.32578275995361 39.0 36.0 41.0 32.5 41.0 46-47 37.14742945496714 38.5 36.5 41.0 32.5 41.0 48-49 36.979847957737405 38.0 36.0 41.0 32.5 41.0 50-51 36.88366189923979 38.0 36.0 41.0 31.0 41.0 52-53 36.54532921015333 38.0 35.5 41.0 31.0 41.0 54-55 36.57006829016879 38.0 36.5 41.0 31.0 41.0 56-57 36.440793712150494 37.5 35.5 40.0 30.5 41.0 58-59 36.162208478288875 37.5 35.5 40.0 30.5 41.0 60-61 35.98141992011339 37.0 35.0 40.0 30.0 41.0 62-63 35.763548511789715 36.5 35.0 40.0 29.0 41.0 64-65 35.52225228707641 37.0 35.0 39.5 29.0 41.0 66-67 35.21257569900786 36.5 34.5 39.0 29.0 41.0 68-69 35.018348151011466 37.0 34.0 39.0 29.0 40.5 70-71 34.61252415925783 36.0 34.0 37.5 28.0 40.0 72-73 34.337959025898726 36.0 34.0 37.0 27.5 39.5 74-75 34.072567968045355 36.0 34.0 37.0 27.0 39.0 76-77 32.47946140961216 34.5 31.0 36.5 25.5 37.5 78-79 33.391367091869604 35.0 33.0 36.0 26.0 38.5 80-81 33.42887514495555 35.0 33.5 36.0 27.0 37.5 82-83 33.234634712021645 35.0 33.5 36.0 26.0 37.5 84-85 33.17292874629558 35.0 33.0 36.5 26.5 37.0 86-87 32.87299317098312 35.0 33.0 36.0 26.0 36.5 88-89 32.89268135549543 35.0 33.0 35.5 26.0 37.0 90-91 32.74920757634325 35.0 33.0 36.0 26.0 37.0 92-93 32.552647854657906 35.0 33.0 35.0 25.0 37.0 94-95 32.48314650173947 35.0 33.0 35.0 25.0 36.0 96-97 32.26417987372761 35.0 32.5 35.0 24.5 36.5 98-99 32.204574152815354 35.0 32.5 35.0 24.5 36.0 100 31.066022419791263 34.0 30.0 35.0 21.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05179647261541476 1101 2 -0.11563943239019636 1101 3 -0.14500303482687826 1101 4 0.02331555233079996 1101 5 0.18096245007096456 1101 6 0.34748217550379934 1101 7 0.00997102262800098 1101 8 0.08645174824704327 1101 9 -0.41721007086897544 1101 10-11 0.268697392611287 1101 12-13 0.12206982324426718 1101 14-15 -0.2395604122910484 1101 16-17 -0.5212592338695003 1101 18-19 -0.44848067798775304 1101 20-21 -1.225734136975106 1101 22-23 -0.301049169617464 1101 24-25 0.04545739671176108 1101 26-27 -0.8140549382617479 1101 28-29 -0.12794106754196832 1101 30-31 -0.26096986483949536 1101 32-33 0.3910175669379754 1101 34-35 -0.24530730504747567 1101 36-37 -1.0776257670621874 1101 38-39 -0.7337132518894052 1101 40-41 -1.070489957078074 1101 42-43 0.16146086873578724 1101 44-45 -0.42931221757332594 1101 46-47 -0.5650214957585362 1101 48-49 -0.31784867951326845 1101 50-51 -0.3266245680093789 1101 52-53 -1.0536020940117439 1101 54-55 -1.2649278906871047 1101 56-57 -1.5455352845825132 1101 58-59 -2.665526928930639 1101 60-61 -1.5020370785045785 1101 62-63 -1.2619610626447226 1101 64-65 -1.7877271972140818 1101 66-67 -0.7777502567817223 1101 68-69 -1.8335470369535756 1101 70-71 -2.043458767235535 1101 72-73 -0.8910873284529899 1101 74-75 -0.7585865028497452 1101 76-77 -1.3114956123987653 1101 78-79 -1.6857294276158612 1101 80-81 -0.4654452126829298 1101 82-83 -1.476572062134661 1101 84-85 -1.6379894531978678 1101 86-87 -1.6161872503618753 1101 88-89 -1.7349864002131028 1101 90-91 -1.879577676404466 1101 92-93 -1.3020656546595752 1101 94-95 -1.674055037476343 1101 96-97 -0.34309170035102454 1101 98-99 -1.2888973150663823 1101 100 -2.239757776262845 1102 1 -0.5672511464322056 1102 2 -0.16325848000924736 1102 3 -0.16881255863640376 1102 4 0.49950602852127446 1102 5 0.39524816435667987 1102 6 0.41891074693236874 1102 7 -0.2757432630862837 1102 8 0.4435946053899045 1102 9 0.6304089767500685 1102 10-11 0.5306021545160462 1102 12-13 0.050641251815697785 1102 14-15 0.2961538734232363 1102 16-17 0.6335026708923976 1102 18-19 0.32532884582177246 1102 20-21 -0.05906747030844173 1102 22-23 0.3537127351444411 1102 24-25 0.5454573967117611 1102 26-27 0.5073736331668215 1102 28-29 0.2768208372199368 1102 30-31 0.905696801827176 1102 32-33 1.0576842336046468 1102 34-35 0.49278793304776514 1102 36-37 0.6128504234140024 1102 38-39 0.825810557634405 1102 40-41 0.6199862333981159 1102 42-43 0.31622277349769234 1102 44-45 0.5706877824266741 1102 46-47 0.06593088519384338 1102 48-49 -1.3535629652275531 1102 50-51 -1.576624568009379 1102 52-53 -0.9940782844879337 1102 54-55 -0.6696897954490098 1102 56-57 -0.7479162369634693 1102 58-59 -0.2726697860735001 1102 60-61 -0.22822755469506006 1102 62-63 -0.6548182055018614 1102 64-65 -0.6091557686426512 1102 66-67 -0.8491788282102917 1102 68-69 -1.0954517988583348 1102 70-71 -0.9125063862831553 1102 72-73 -0.3315635189291797 1102 74-75 -1.151443645706884 1102 76-77 -1.0257813266844806 1102 78-79 -1.2452532371396714 1102 80-81 -1.2987785460162584 1102 82-83 -1.5360958716584676 1102 84-85 -0.9832275484359627 1102 86-87 -0.44952058369520387 1102 88-89 -1.4968911621178655 1102 90-91 -1.7605300573568456 1102 92-93 -2.0401608927548125 1102 94-95 -3.864531227952533 1102 96-97 -2.7121393193986414 1102 98-99 -2.1103258864949552 1102 100 -0.5730911095961808 1103 1 0.30179647261541476 1103 2 0.39229707554630977 1103 3 0.18436204453819727 1103 4 -0.23462095560570617 1103 5 0.4150894341979452 1103 6 0.6054186834403055 1103 7 -0.5455845329275562 1103 8 -0.33815142635612716 1103 9 0.18993278627387866 1103 10-11 -0.1003502264363334 1103 12-13 0.005006331180773316 1103 14-15 -0.8586080313386617 1103 16-17 -0.21173542434569015 1103 18-19 0.15072567121860203 1103 20-21 0.12942459318361443 1103 22-23 0.3398238462555554 1103 24-25 -0.10930450805014402 1103 26-27 -0.6989755731823877 1103 28-29 -0.3759569405578347 1103 30-31 -0.7252555791252036 1103 32-33 -0.6942998933794797 1103 34-35 -0.8087993685395389 1103 36-37 -0.11135592579234554 1103 38-39 -0.6920465852227338 1103 40-41 -0.5228709094590229 1103 42-43 -1.5250470677721566 1103 44-45 -1.0166138048749076 1103 46-47 -1.354704035441074 1103 48-49 -0.9607058223704072 1103 50-51 -0.5825769489617656 1103 52-53 -0.9266179670276173 1103 54-55 -1.056594557353776 1103 56-57 -0.7895829036301336 1103 58-59 -0.13774915115286035 1103 60-61 -0.7956878721553764 1103 62-63 -0.6687070943907543 1103 64-65 -0.46828275276963893 1103 66-67 -1.4146550186864815 1103 68-69 -0.7720391004456388 1103 70-71 -1.12877622755299 1103 72-73 -1.5002143125799705 1103 74-75 -1.703030947294188 1103 76-77 -0.18847973938289186 1103 78-79 -0.06866593555236733 1103 80-81 -1.431715053952768 1103 82-83 -0.15712761769022165 1103 84-85 -1.901878342086757 1103 86-87 -1.0923777265523498 1103 88-89 -0.49887528910199563 1103 90-91 -0.22481577164256095 1103 92-93 -0.14928787688180023 1103 94-95 -0.11453122795253279 1103 96-97 -0.49785360511292964 1103 98-99 -1.0627068388759042 1103 100 -1.6683292048342757 1104 1 0.016912751685183025 1104 2 -0.26790964279994256 1104 3 0.026093310688068527 1104 4 -0.3166622992749595 1104 5 0.5320144987974302 1104 6 0.08668483331111076 1104 7 0.17663768929466528 1104 8 0.22709405168004793 1104 9 0.2848940265839559 1104 10-11 -0.21662929620377724 1104 12-13 0.6616600779507991 1104 14-15 0.6549578601342034 1104 16-17 0.13322581596438 1104 18-19 0.20821921127028276 1104 20-21 0.29641425726630644 1104 22-23 -0.260628350126872 1104 24-25 0.030230375670782905 1104 26-27 -0.2351512837767018 1104 28-29 0.18379758140598312 1104 30-31 0.6410234906422403 1104 32-33 0.5812169024861475 1104 34-35 0.6802187193157607 1104 36-37 0.8941350302800046 1104 38-39 0.5104727946222241 1104 40-41 0.27364071844795745 1104 42-43 0.5343844567756477 1104 44-45 0.7708538953834889 1104 46-47 0.7602830446622875 1104 48-49 -0.563419000664986 1104 50-51 -1.686259119504392 1104 52-53 -0.3562045303351127 1104 54-55 -0.9451604488266412 1104 56-57 -0.6122573222347825 1104 58-59 -0.09575948707017545 1104 60-61 -0.5598997584602827 1104 62-63 -0.6586941744941157 1104 64-65 0.07855187255336205 1104 66-67 0.47972482627475443 1104 68-69 0.5939169719057844 1104 70-71 0.08278707994054457 1104 72-73 0.538868374758529 1104 74-75 0.0011034196308798982 1104 76-77 0.2779285293509588 1104 78-79 0.5537500851194679 1104 80-81 0.1403133698198431 1104 82-83 0.5970713487180532 1104 84-85 0.2922431049416687 1104 86-87 -0.2726102846918863 1104 88-89 0.6816802664535615 1104 90-91 -0.09690879489837556 1104 92-93 0.5777793065807373 1104 94-95 0.2633757487916526 1104 96-97 0.33547972822040606 1104 98-99 0.2260528510465747 1104 100 0.3239188571812299 1105 1 0.42679647261541476 1105 2 0.024241519990752636 1105 3 -0.06911017768402417 1105 4 0.31051793328317956 1105 5 -0.06754945469094054 1105 6 0.13319646121808404 1105 7 -0.13586231070533472 1105 8 0.13407079586609427 1105 9 0.14826611960721436 1105 10-11 0.6496497735636666 1105 12-13 0.04320077562521618 1105 14-15 -0.4158996980053331 1105 16-17 0.21534790898763845 1105 18-19 -0.022885439892512238 1105 20-21 0.33602181540584297 1105 22-23 0.7651710684777768 1105 24-25 0.21882049194985598 1105 26-27 1.110052204595391 1105 28-29 0.7143208372199368 1105 30-31 0.27474442087479645 1105 32-33 -0.071036004490594 1105 34-35 -0.5414382574284247 1105 36-37 -0.4012864813479027 1105 38-39 0.06316174811059483 1105 40-41 0.21150409054097707 1105 42-43 0.21280015445007194 1105 44-45 -0.27529436043046474 1105 46-47 -0.4675512576632954 1105 48-49 0.24762751096292135 1105 50-51 0.6465897177049058 1105 52-53 0.7313681440834969 1105 54-55 0.15694710931289535 1105 56-57 1.2572920963698664 1105 58-59 -1.165526928930639 1105 60-61 0.05674268340018074 1105 62-63 0.22539012783146717 1105 64-65 -0.4596021972140818 1105 66-67 0.19992831464684713 1105 68-69 -0.780719656001196 1105 70-71 0.3694876613358957 1105 72-73 0.08138290964225092 1105 74-75 0.12683016381692624 1105 76-77 0.0024924828393295684 1105 78-79 0.0962646200031898 1105 80-81 -0.18345116506387882 1105 82-83 0.2525946045320069 1105 84-85 -0.3254894531978678 1105 86-87 0.0013722734476502296 1105 88-89 -2.4745697335464385 1105 90-91 0.07205922835743905 1105 92-93 -0.015607321326243095 1105 94-95 -1.0989062279525328 1105 96-97 -0.9457702717795939 1105 98-99 -0.11479017220923993 1105 100 -0.34541253816760786 1106 1 -0.42494771343109505 1106 2 0.034415938595401485 1106 3 0.18888400836248564 1106 4 0.24147723560876244 1106 5 0.06689821972766197 1106 6 0.13319646121808404 1106 7 -0.19545533396114934 1106 8 -0.09848734366878631 1106 9 -0.41278039202069294 1106 10-11 0.43453349449389833 1106 12-13 0.2779391477182358 1106 14-15 0.18984158106443516 1106 16-17 0.1913653508481019 1106 18-19 0.010544792665626801 1106 20-21 0.21501890842910143 1106 22-23 -0.4931864896617597 1106 24-25 0.16976525939171694 1106 26-27 0.009034762734927426 1106 28-29 0.1489138604757514 1106 30-31 -0.01013930005544239 1106 32-33 -0.15134123704873303 1106 34-35 0.5174280216413365 1106 36-37 0.4406466581869779 1106 38-39 -0.10580627514521979 1106 40-41 0.22712909054097707 1106 42-43 0.19717515445007194 1106 44-45 0.36387715119744257 1106 46-47 0.5858644400111217 1106 48-49 -0.284349233223125 1106 50-51 -0.058352142760206505 1106 52-53 0.22519081850209943 1106 54-55 0.14786280698731957 1106 56-57 -0.623885229211524 1106 58-59 -0.107387394046917 1106 60-61 -0.6529230142742435 1106 62-63 -0.21683370937783053 1106 64-65 -0.9563318483768768 1106 66-67 0.0029806402282446243 1106 68-69 -0.49910628390817635 1106 70-71 -0.5451198968036408 1106 72-73 -0.47275953221821254 1106 74-75 -0.8244779757179614 1106 76-77 -0.617420307858346 1106 78-79 -0.05090107767123442 1106 80-81 -0.022477327854574014 1106 82-83 0.3761411161599142 1106 84-85 0.1527082212207418 1106 86-87 0.08785483158718677 1106 88-89 1.1351686385465811 1106 90-91 0.030998181845809825 1106 92-93 0.5428955856504984 1106 94-95 -0.2598800651618376 1106 96-97 0.312223914266923 1106 98-99 0.5051226184884356 1106 100 1.0681049036928556 1107 1 -0.010703527384585243 1107 2 -1.1007584800092474 1107 3 -0.31911017768402417 1107 4 0.02926793328317956 1107 5 -0.036299454690940536 1107 6 -0.11680353878191596 1107 7 0.3016376892946653 1107 8 0.25907079586609427 1107 9 0.39826611960721436 1107 10-11 0.2277747735636666 1107 12-13 -0.05054922437478382 1107 14-15 -0.08777469800533311 1107 16-17 -0.44090209101236155 1107 18-19 -0.46038543989251224 1107 20-21 0.22664681540584297 1107 22-23 0.40579606847777683 1107 24-25 0.06257049194985598 1107 26-27 0.01630220459539089 1107 28-29 -0.3325541627800632 1107 30-31 0.7747444208747964 1107 32-33 -0.555411004490594 1107 34-35 0.25543674257157534 1107 36-37 -0.21378648134790268 1107 38-39 0.5319117481105948 1107 40-41 0.8833790905409771 1107 42-43 0.9628001544500719 1107 44-45 -0.025294360430464735 1107 46-47 -0.5613012576632954 1107 48-49 0.15387751096292135 1107 50-51 0.7247147177049058 1107 52-53 0.9657431440834969 1107 54-55 -0.09305289068710465 1107 56-57 0.8354170963698664 1107 58-59 1.146973071069361 1107 60-61 1.7754926834001807 1107 62-63 1.4128901278314672 1107 64-65 0.4778978027859182 1107 66-67 0.6999283146468471 1107 68-69 0.766155343998804 1107 70-71 0.9319876613358957 1107 72-73 1.159507909642251 1107 74-75 -0.26379483618307376 1107 76-77 0.37749248283932957 1107 78-79 -0.4193603799968102 1107 80-81 0.3165488349361212 1107 82-83 0.14321960453200688 1107 84-85 -0.20048945319786782 1107 86-87 0.01699727344765023 1107 88-89 -0.1308197335464385 1107 90-91 0.040809228357439054 1107 92-93 -0.2343573213262431 1107 94-95 0.3854687720474672 1107 96-97 -0.5082702717795939 1107 98-99 0.4633348277907601 1107 100 0.6858374618323921 1108 1 0.20893932975827312 1108 2 -0.034687051437821026 1108 3 0.5549969651731175 1108 4 0.4328393618546116 1108 5 0.21905768816620252 1108 6 0.5331964612180826 1108 7 0.490923403580382 1108 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-1.7019262576632954 2316 48-49 -1.7637361254007118 2316 50-51 -0.49545573684054744 2316 52-53 -0.9405068559165031 2316 54-55 -2.2422006179598313 2316 56-57 -2.6645829036301336 2316 58-59 -1.3246178380215454 2316 60-61 -1.285586862054366 2316 62-63 -0.8669394176230796 2316 64-65 -0.5036362881231753 2316 66-67 0.350496496465027 2316 68-69 -0.4980492014557427 2316 70-71 -0.5012509750277374 2316 72-73 -1.3120829994486556 2316 74-75 -0.296465290728527 2316 76-77 -0.057166608069763925 2316 78-79 -1.7062921981786303 2316 80-81 0.08217383493612118 2316 82-83 -0.7644508500134464 2316 84-85 -0.5811712713796879 2316 86-87 0.03262227344765023 2316 88-89 -0.7615015517282586 2316 90-91 -0.8384521352789243 2316 92-93 -0.8210050485989697 2316 94-95 -1.250894864316166 2316 96-97 -0.9258839081432271 2316 98-99 -0.7937674449365133 2316 100 0.6574283709233022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 1.0 14 2.0 15 6.0 16 15.0 17 39.0 18 46.0 19 91.0 20 102.0 21 155.0 22 197.0 23 259.0 24 256.0 25 285.0 26 335.0 27 382.0 28 506.0 29 614.0 30 746.0 31 913.0 32 1179.0 33 1569.0 34 2312.0 35 4178.0 36 7731.0 37 6742.0 38 8334.0 39 1808.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50908809652719 19.761775852734164 13.950550442158455 23.778585608580194 2 15.511687240677267 21.003530654846276 39.15418910908951 24.330592995386954 3 20.278314650173947 24.491689215307304 29.779667568612293 25.450328565906453 4 12.776704032985439 15.791779409869863 38.51050122406907 32.92101533307564 5 13.549800283468624 37.42301249838938 34.90014173431259 14.127045483829404 6 31.539750032212343 36.83030537301894 17.010694498131684 14.619250096637032 7 27.3057595670661 32.30253833268908 21.83223811364515 18.559463986599663 8 26.068805566293 33.910578533694114 20.033500837520936 19.98711506249195 9 25.64360262852725 16.49014302280634 20.456126787785077 37.41012756088133 10-11 25.278958897049346 25.67710346604819 27.839195979899493 21.204741657002963 12-13 26.634454322896534 23.13748228321093 27.56474681097797 22.66331658291457 14-15 23.022398639070033 24.965847874835685 26.42601232054025 25.585741165554037 16-17 22.769266451055934 27.883364042830085 26.518831578811735 22.828537927302246 18-19 22.220074732637546 27.470686767169177 28.04020100502513 22.26903749516815 20-21 23.7624017523515 25.857492591160934 27.25293132328308 23.127174333204483 22-23 23.236995709260526 25.78309216714556 27.719723227976132 23.260188895617777 24-25 23.26117768328824 25.95284112872053 27.5737662672336 23.212214920757635 26-27 22.824854388949024 25.55924952322045 28.84129684036905 22.77459924746147 28-29 22.5588227714352 27.110017266706183 27.823879596938383 22.507280364920238 30-31 22.973443117244578 25.58918654245107 27.828675248366768 23.608695091937584 32-33 23.056206994304564 25.867845269695643 27.850939360358733 23.22500837564106 34-35 23.83676730191864 25.590475086010283 27.73590011210329 22.836857499967785 36-37 23.363269723872232 25.646510069708413 28.128181009934416 22.862039196484943 38-39 22.93132328308208 26.282695528926688 27.987372761242106 22.79860842674913 40-41 23.11716424641472 26.446674999033615 27.529023695705394 22.90713705884627 42-43 22.84330021776386 26.255363562565233 26.89448116793588 24.006855051735023 44-45 23.094268632099784 25.845273683125452 27.20466962168849 23.85578806308628 46-47 23.587093781408655 26.168079787645286 27.140942710615157 23.103883720330902 48-49 23.333462197652093 26.284455097228125 27.20454633316581 23.17753637195397 50-51 22.127007035901137 26.72740393288833 27.882013350171388 23.263575681039146 52-53 23.19468068656255 26.936755837327976 26.505077057883614 23.363486418225865 54-55 22.64714977837336 25.954798474384084 27.23044016080816 24.167611586434386 56-57 23.425118220824903 26.123258900385267 26.93888595394864 23.51273692484119 58-59 24.172765694258324 25.62364704669622 26.958560973095558 23.2450262859499 60-61 22.850737037418824 26.31172044119163 28.165910730852488 22.67163179053706 62-63 22.930034789331273 26.295580466434735 27.657518361035947 23.116866383198044 64-65 22.44427264527767 27.268393248292742 27.04419533565262 23.243138770776962 66-67 23.283082077051926 26.024996778765626 26.700167504187604 23.991753639994844 68-69 22.538332689086456 26.34454322896534 27.147274835717045 23.969849246231156 70-71 23.503414508439636 26.231155778894472 26.725937379203714 23.539492333462185 72-73 22.8461716877561 25.697755328196276 27.603535809086928 23.852537174960702 74-75 22.57158320661839 27.251874951676502 26.84724620499472 23.329295636710395 76-77 22.893149837637235 26.112056079583528 26.46513066336787 24.52966341941137 78-79 22.718896978287482 26.293408929836996 27.088460795051866 23.899233296823656 80-81 23.34394206857452 26.161914210980687 26.605161772474844 23.88898194796995 82-83 22.854897109796795 26.256652106124445 27.318411998917625 23.57003878516113 84-85 22.940638328028964 26.477599247509954 27.161798245048903 23.41996417941218 86-87 22.4507151140317 27.05708027316068 26.54812524159258 23.94407937121505 88-89 22.228092102720044 26.69278047649113 26.335863108660078 24.743264312128748 90-91 22.774420492468657 25.88617299540002 26.63995155201072 24.699454960120605 92-93 23.401623502126014 26.826439891766523 26.442468754026542 23.329467852080917 94-95 22.926169308078855 26.097152428810723 27.01713696688571 23.959541296224714 96-97 21.62719377367729 26.22477643481174 28.02360642218385 24.124423369327115 98-99 22.508278892367954 26.689602742020696 26.41772005102633 24.384398314585024 100 22.476484989047805 25.491560365932227 26.50173946656359 25.530215178456384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 3.5 2 4.5 3 5.0 4 2.5 5 3.0 6 6.0 7 16.5 8 26.5 9 31.5 10 28.0 11 19.5 12 18.5 13 20.5 14 22.0 15 21.0 16 25.0 17 25.0 18 25.5 19 30.0 20 35.0 21 38.0 22 40.0 23 53.5 24 64.5 25 71.0 26 100.0 27 138.0 28 164.0 29 201.0 30 235.0 31 313.5 32 412.5 33 480.0 34 575.0 35 685.0 36 793.0 37 883.5 38 985.5 39 1070.5 40 1134.0 41 1195.0 42 1240.0 43 1298.0 44 1354.0 45 1421.5 46 1492.5 47 1778.5 48 2322.5 49 2262.0 50 1719.0 51 1525.0 52 1485.5 53 1383.5 54 1302.0 55 1230.0 56 1134.5 57 1032.5 58 914.0 59 802.5 60 679.0 61 575.5 62 481.5 63 359.0 64 243.0 65 175.5 66 144.5 67 111.5 68 77.0 69 53.5 70 56.0 71 43.0 72 28.0 73 25.0 74 12.0 75 10.5 76 11.0 77 6.5 78 5.5 79 3.5 80 1.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04638577502899111 2 0.005153975003221235 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.02061590001288494 16-17 0.0012884937508053087 18-19 0.0 20-21 0.0 22-23 0.0012884937508053087 24-25 0.0 26-27 0.007730962504831851 28-29 0.005153975003221235 30-31 0.0038654812524159254 32-33 0.005153975003221235 34-35 0.0038654812524159254 36-37 0.0012884937508053087 38-39 0.0 40-41 0.0012884937508053087 42-43 0.0038654812524159254 44-45 0.0025769875016106174 46-47 0.005153975003221235 48-49 0.011596443757247778 50-51 0.01030795000644247 52-53 0.007730962504831851 54-55 0.0025769875016106174 56-57 0.0012884937508053087 58-59 0.0025769875016106174 60-61 0.0025769875016106174 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.005153975003221235 74-75 0.01030795000644247 76-77 0.007730962504831851 78-79 0.006442468754026543 80-81 0.0012884937508053087 82-83 0.0038654812524159254 84-85 0.0012884937508053087 86-87 0.0 88-89 0.0012884937508053087 90-91 0.0012884937508053087 92-93 0.0 94-95 0.0 96-97 0.005153975003221235 98-99 0.0038654812524159254 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 38805.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.05038010565649 #Duplication Level Percentage of deduplicated Percentage of total 1 94.86341011691168 84.47622729029764 2 4.265539993054752 7.5969591547481 3 0.4716981132075472 1.2601468882875917 4 0.17941891422618358 0.6390929003994331 5 0.06655862947100359 0.29635356268522095 6 0.023150827642088204 0.12369540007730961 7 0.023150827642088204 0.14431130009019455 8 0.0028938534552610255 0.02061590001288494 9 0.011575413821044102 0.09277155005798222 >10 0.04630165528417641 0.9637933256023709 >50 0.03183238800787128 1.9920113387450071 >100 0.014469267276305127 2.394021388996263 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 347 0.8942146630588841 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 210 0.5411673753382297 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 153 0.39427908774642445 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 113 0.2911995876819997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 106 0.27316067517072545 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 97 0.24996778765622985 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 94 0.242236825151398 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.21904393763690247 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 84 0.21646695013529185 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 69 0.17781213761113257 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.17265816260791134 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 63 0.1623502126014689 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57 0.14688828759180517 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 55 0.14173431258858393 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 51 0.1314263625821415 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 51 0.1314263625821415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0012884937508053087 0.0 32-33 0.0 0.0 0.0 0.012884937508053085 0.0 34-35 0.0 0.0 0.0 0.029635356268522095 0.0 36-37 0.0 0.0 0.0 0.04896276253060173 0.0 38-39 0.0 0.0 0.0 0.06571318129107073 0.0 40-41 0.0 0.0 0.0 0.10694498131684062 0.0 42-43 0.0 0.0 0.0 0.14817678134261048 0.0 44-45 0.0 0.0 0.0 0.18683159386676976 0.0 46-47 0.0 0.0 0.0 0.25383326890864577 0.0 48-49 0.0 0.0 0.0 0.3195464501997165 0.0 50-51 0.0 0.0 0.0 0.383971137739982 0.0 52-53 0.0 0.0 0.0 0.4329339002705837 0.0 54-55 0.0 0.0 0.0 0.5050895503156809 0.0 56-57 0.0 0.0 0.0 0.6030150753768844 0.0 58-59 0.0 0.0 0.0 0.7112485504445304 0.0 60-61 0.0 0.0 0.0 0.8310784692694241 0.0 62-63 0.0 0.0 0.0 0.985697719366061 0.0 64-65 0.0 0.0 0.0 1.1519134132199458 0.0 66-67 0.0 0.0 0.0 1.2897822445561138 0.0 68-69 0.0 0.0 0.0 1.4959412446849634 0.0 70-71 0.0 0.0 0.0 1.6840613323025384 0.0 72-73 0.0 0.0 0.0 1.899239788687025 0.0 74-75 0.0 0.0 0.0 2.1453420950908386 0.0 76-77 0.0 0.0 0.0 2.3695400077309623 0.0 78-79 0.0 0.0 0.0 2.622084782888803 0.0 80-81 0.0 0.0 0.0 2.9622471331014046 0.0 82-83 0.0 0.0 0.0 3.2959670145599795 0.0 84-85 0.0 0.0 0.0 3.6142249710088907 0.0 86-87 0.0 0.0 0.0 3.9492333462182705 0.0 88 0.0 0.0 0.0 4.21852854013658 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGA 15 6.819507E-4 94.11096 1 ACTGTGA 25 0.0016931783 37.59588 16-17 TGTTGGT 35 0.00879096 26.854198 44-45 GTACATG 145 6.39266E-4 19.471235 1 AGAGTAC 150 4.0017767E-9 18.822193 10-11 ACATCTC 155 0.0010097931 18.191553 94 TACTTTT 65 0.007948754 18.086596 14-15 TACATGG 165 0.0015357495 17.089033 2 ACATGGG 145 0.0048711468 16.20512 3 GAGTACA 125 0.0015857082 13.175535 12-13 AGTACAT 130 0.0021299853 12.668785 12-13 AGTGGTA 445 0.0010457615 9.516839 9 CAGTGGT 455 0.0012814983 9.307678 8 GCAGTGG 465 0.0015813925 9.095777 7 TAAGCAG 425 0.00782345 8.846088 4 GTAAGCA 430 0.008610636 8.743227 3 GAGTAAG 435 0.009365688 8.653882 1 AGTAAGC 435 0.0094655035 8.642731 2 CAACGCA 510 0.0036572784 8.293208 4 TCAACGC 520 0.0043577175 8.133723 3 >>END_MODULE