FastQCFastQC Report
Mon 6 Jun 2016
ERR523074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9979729
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245840.24633935450551814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204580.2049955464722539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118010.11824970397492757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136600.049.638021
GTATCAA247350.041.1096731
TCAACGC298450.033.586264
CAACGCA303050.033.0442775
ATCAACG307950.032.5836223
TATCAAC317450.031.7600842
AACGCAG317950.031.6382876
GTACATG349000.028.273151
ACGCAGA368600.027.1892497
TACATGG361600.026.712412
CGCAGAG386200.025.8762688
ACATGGG367850.025.5529423
CATGGGG267000.023.989824
GCAGAGT428750.023.0983099
GAGTACT232750.020.53925112-13
ATGGGGG151000.020.072895
CAGAGTA413750.019.83073610-11
GTGGTAT76900.019.7029271
AGAGTAC385300.019.56970610-11
GTACTTT253300.018.69722614-15
Produced by FastQC (version 0.11.5)