FastQCFastQC Report
Mon 6 Jun 2016
ERR523074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9979729
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213370.2138034008739115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186250.186628314255828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107830.10804902618097145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135250.051.29741
GTATCAA244350.041.098521
TCAACGC296750.033.428424
CAACGCA303350.032.7630775
ATCAACG308000.032.222993
AACGCAG317350.031.3767976
TATCAAC319300.031.3402372
GTACATG342550.027.9297871
ACGCAGA371950.026.7202037
TACATGG353500.026.4856992
CGCAGAG387300.025.6557438
ACATGGG363950.024.9710853
CATGGGG261200.022.8660284
GCAGAGT437350.022.526279
ATGGGGG150800.020.3483945
GAGTACT239700.020.17808312-13
GTGGTAT73450.019.7242071
CAGAGTA421050.019.58066410-11
AGAGTAC395300.019.3638810-11
GTACTTT261050.018.3005914-15
Produced by FastQC (version 0.11.5)