FastQCFastQC Report
Mon 6 Jun 2016
ERR523073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4451053
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123460.277372567794632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106220.23864015998012156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57990.1302837777937041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG86600.055.4919172
GTACCTG101600.048.1910481
ACCTGGG98500.048.167233
TATAACG4800.042.123452
CCTGGGG90150.038.0732154
ATAACGC6450.032.07673
TAACGCA6700.030.1756134
CTGGGGG57550.027.4518175
GGTATCA134350.027.2364981
CATGGGG126600.026.9628224
GTATCAA186400.026.6457161
TGGGGGG89150.026.5681386
GTACATG198000.026.41491
ATGGGGG80850.026.0540435
TACATGG204000.025.4930442
GTACCCG8500.024.9002061
ACATGGG204250.024.6560843
GTATAAC12700.022.9613441
TCAACGC213750.022.8105134
CAACGCA215300.022.647065
Produced by FastQC (version 0.11.5)