FastQCFastQC Report
Mon 6 Jun 2016
ERR523073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4451053
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102480.23023765387650968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97220.21842022550618923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53040.1191628138330413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG88850.059.4028662
ACCTGGG97950.053.6849063
GTACCTG102400.052.1566731
CCTGGGG89950.040.64484
TATAACG4650.038.4074752
CTGGGGG53150.032.4481055
TAACGCA5400.031.3281884
ATAACGC6250.028.571313
TGGGGGG77650.027.8383146
GTATCAA180750.025.2304461
ATGGGGG75300.024.9627025
GGTATCA132500.024.5894531
GTACATG193100.023.9576551
CATGGGG122050.023.7560334
TACATGG199850.023.0230922
GTATAAC11400.022.6824171
GTACCCG8100.022.0561681
ACATGGG200150.021.9993843
TCAACGC208650.021.5761344
CAACGCA210650.021.3489765
Produced by FastQC (version 0.11.5)