FastQCFastQC Report
Mon 6 Jun 2016
ERR523072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5941116
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199010.3349707361377896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163290.27484735191166104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105490.1775592329791238No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70630.11888338823884267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62870.10582186915724251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG125400.057.968862
GTACCTG138200.052.987491
ACCTGGG138950.051.437383
CCTGGGG120400.039.324224
GTACCCG12750.029.879111
CTGGGGG70900.028.712645
TACCCGG15400.025.0366332
TGGGGGG97850.023.6766156
CATGGGG150100.023.1170714
ATGGGGG90850.022.1488065
GTACATG299600.021.4124011
GTATCAA336450.021.1779981
TACATGG304350.020.8874872
GTACCGG9600.020.0865541
ACATGGG304000.019.9994833
GTACACG13500.019.8579251
TAACGCA7650.019.6673434
TATCACG5750.019.6257482
GAGTACT178450.019.44495812-13
ATAACGC7850.019.1678833
Produced by FastQC (version 0.11.5)