##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523072_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5941116 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.340784626996005 33.0 31.0 34.0 28.0 34.0 2 31.68359412608675 34.0 31.0 34.0 27.0 35.0 3 31.661740824451165 34.0 31.0 34.0 27.0 36.0 4 33.95838677447133 35.0 34.0 37.0 31.0 37.0 5 34.050831863912435 35.0 34.0 37.0 31.0 37.0 6 34.27201572903138 35.0 35.0 37.0 30.0 37.0 7 34.15816220386877 35.0 34.0 37.0 30.0 37.0 8 34.26189759634385 36.0 34.0 37.0 30.0 37.0 9 35.43309253682305 37.0 35.0 39.0 30.0 39.0 10-11 35.39410196670121 37.0 34.5 39.0 30.0 39.0 12-13 35.42181511015775 37.0 35.0 39.0 30.0 39.0 14-15 36.18779973324877 38.0 35.0 41.0 30.0 41.0 16-17 36.34543526839065 38.0 36.0 40.5 30.0 41.0 18-19 36.20658559772272 38.0 35.5 40.0 29.5 41.0 20-21 36.30655107558917 38.0 35.0 40.0 30.0 41.0 22-23 36.15027488101562 38.0 35.0 40.0 29.5 41.0 24-25 36.12822885464617 38.0 35.0 40.0 29.5 41.0 26-27 36.011963156417075 38.0 36.0 40.0 29.5 41.0 28-29 35.78053946430266 38.0 35.0 40.0 29.0 41.0 30-31 35.59726085132827 37.0 35.0 40.0 28.0 41.0 32-33 35.56887510696644 37.5 34.5 40.0 27.0 41.0 34-35 35.414465396736915 37.5 34.5 40.0 27.0 41.0 36-37 35.23263979360107 37.5 34.5 40.0 27.0 41.0 38-39 35.07015971410085 36.5 34.5 40.0 26.5 41.0 40-41 35.020904237520355 37.0 34.0 40.0 25.5 41.0 42-43 34.81088376325256 36.0 34.5 40.0 25.0 41.0 44-45 34.73225686891149 37.0 33.5 40.0 25.0 41.0 46-47 34.51775836391681 36.5 33.5 40.0 24.0 41.0 48-49 34.38161634952087 37.0 33.0 40.0 23.0 41.0 50-51 33.358350686975314 36.5 31.5 38.5 22.0 40.0 52-53 33.57747668956472 36.5 32.5 38.5 22.5 39.5 54-55 34.43160939796496 37.0 33.0 39.5 24.0 41.0 56-57 34.57113057546764 37.0 34.0 40.0 24.5 41.0 58-59 34.50266414592814 37.0 33.0 40.0 23.5 41.0 60-61 34.249475182777104 36.5 34.0 39.5 23.5 41.0 62-63 34.07922316278625 36.5 33.0 39.0 23.5 41.0 64-65 33.6809901540384 36.0 33.0 39.0 22.5 41.0 66-67 33.486426960860555 36.0 33.0 38.5 22.5 41.0 68-69 33.146890079237636 35.0 32.5 37.5 21.5 40.0 70-71 32.94101823630443 35.5 32.0 37.5 21.5 40.0 72-73 32.61966068327904 35.0 32.0 37.5 21.0 39.0 74-75 32.242730742843605 35.0 32.0 37.0 20.0 39.0 76-77 32.12128016015846 35.0 31.0 37.0 19.5 38.5 78-79 31.846958803699508 35.0 31.0 36.5 20.0 38.0 80-81 31.579830371936854 35.0 30.0 36.0 18.5 37.5 82-83 31.266241729668298 34.5 30.0 36.0 18.0 37.0 84-85 31.026382585359386 35.0 30.0 35.5 18.0 37.0 86-87 30.790748825641515 34.0 29.5 35.0 17.5 37.0 88-89 30.631654810308365 34.0 29.5 35.0 17.5 36.5 90-91 30.416629889064613 34.0 29.0 35.0 17.0 36.0 92-93 30.15849160326107 34.0 29.0 35.0 17.0 36.0 94-95 30.06172636588816 34.0 29.0 35.0 16.0 36.0 96-97 29.87651890654887 34.0 29.0 35.0 17.0 36.0 98-99 29.785430296260838 34.0 28.5 35.0 16.0 36.0 100 28.54250901682445 33.0 25.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0364613658598536 1101 2 0.041249536076385596 1101 3 0.09374613199060633 1101 4 0.06966385802377317 1101 5 0.05295814137407717 1101 6 0.08796135708291786 1101 7 0.05205146283540785 1101 8 0.04690694985637833 1101 9 0.0853643897249583 1101 10-11 0.06406472238979433 1101 12-13 0.03812149568367573 1101 14-15 -0.04121696659269247 1101 16-17 -0.015701317971370088 1101 18-19 0.03592063898290121 1101 20-21 0.01498511942636327 1101 22-23 0.050002911331723965 1101 24-25 -0.06544829241041583 1101 26-27 0.04666333126166933 1101 28-29 -0.08611270868363619 1101 30-31 -0.16070943532679394 1101 32-33 -0.10849251496212275 1101 34-35 -0.14074993930259438 1101 36-37 -0.12462452364819399 1101 38-39 -0.29368109073760706 1101 40-41 -0.24223084877219492 1101 42-43 -0.1887924527204916 1101 44-45 -0.14513220520633752 1101 46-47 -0.08558725412421353 1101 48-49 -0.1356279329065302 1101 50-51 -0.28802694380544835 1101 52-53 -0.17045322097836646 1101 54-55 -0.10276602864037443 1101 56-57 -0.1591263585086793 1101 58-59 -0.10696477027065043 1101 60-61 -0.05706272046821681 1101 62-63 -0.17372254552722666 1101 64-65 -0.14902593999697444 1101 66-67 -0.18737207717116888 1101 68-69 -0.2102154721982359 1101 70-71 -0.3226272981159539 1101 72-73 -0.17423905022556596 1101 74-75 -0.2546172059109644 1101 76-77 -0.18151648561793365 1101 78-79 -0.3848400244174961 1101 80-81 -0.179131985386082 1101 82-83 -0.49631659941012884 1101 84-85 -0.4689311886357359 1101 86-87 -0.387676113472601 1101 88-89 -0.4587020314368182 1101 90-91 -0.47430645723610354 1101 92-93 -0.3926534065378178 1101 94-95 -0.4005051160887696 1101 96-97 -0.43049761686153687 1101 98-99 -0.40332115818652525 1101 100 -0.4263335979764449 1102 1 0.05156127301439639 1102 2 0.014808692543663682 1102 3 0.08742456613837746 1102 4 -0.012928556472218133 1102 5 -0.020094961992853655 1102 6 -0.0020533106845874727 1102 7 0.09120448242973112 1102 8 0.06113256733915051 1102 9 0.04423817736592639 1102 10-11 0.05001248699178262 1102 12-13 0.021741213397199033 1102 14-15 -0.08599070493786343 1102 16-17 -0.010807077111508079 1102 18-19 0.03178567435188029 1102 20-21 -0.015279822794731501 1102 22-23 0.026171992887540796 1102 24-25 -0.06877344506104777 1102 26-27 -0.19010345425927966 1102 28-29 -0.06363406767497537 1102 30-31 -0.21691423365255957 1102 32-33 -0.20935826103735167 1102 34-35 -0.2130900228332635 1102 36-37 -0.752620997335427 1102 38-39 -0.2889229274445171 1102 40-41 -0.9309218790543383 1102 42-43 -0.9083180940950868 1102 44-45 -0.38939656374100906 1102 46-47 -0.2861805123948713 1102 48-49 -0.2567285447187686 1102 50-51 -0.33417665483531067 1102 52-53 -0.20788828208398513 1102 54-55 -0.2342175630098282 1102 56-57 -0.0666914032529391 1102 58-59 -0.16106046460042478 1102 60-61 -0.2494423011212774 1102 62-63 -0.39141483693493484 1102 64-65 -0.1793635405921563 1102 66-67 -0.10291416459106273 1102 68-69 -0.08521798235226186 1102 70-71 -0.14520043281445538 1102 72-73 -0.07017114904700605 1102 74-75 -0.16999064424737043 1102 76-77 -0.26748741621973693 1102 78-79 -0.29916854309394836 1102 80-81 -0.31401033364424435 1102 82-83 -0.40714349858359355 1102 84-85 -0.49570343298562136 1102 86-87 -0.42843734036330616 1102 88-89 -0.37896751802866646 1102 90-91 -0.3936223646567818 1102 92-93 -0.42550903941418383 1102 94-95 -0.5073875199090416 1102 96-97 -0.6006812210193218 1102 98-99 -0.5305509310327068 1102 100 -0.5352779118727184 1103 1 -0.02100927493473037 1103 2 -0.0816399708475366 1103 3 -0.10549487422735382 1103 4 -0.07923871302230623 1103 5 -0.012769762602793833 1103 6 0.07083851347119463 1103 7 0.04667108158504618 1103 8 0.061856892068071545 1103 9 0.02824846851222418 1103 10-11 -0.027478393177673865 1103 12-13 0.01988535039259176 1103 14-15 -0.02048102714385891 1103 16-17 -0.1016345305439117 1103 18-19 -0.04843966426086865 1103 20-21 -0.15285163088849885 1103 22-23 -0.06430230242402502 1103 24-25 -0.2102902055541378 1103 26-27 -0.19084367499898747 1103 28-29 -0.26863026626610065 1103 30-31 -0.30642907511808914 1103 32-33 -0.32093436833534383 1103 34-35 -0.27280618150727776 1103 36-37 -0.4677522586819407 1103 38-39 -0.3000455504702799 1103 40-41 -0.19824626102088416 1103 42-43 -0.3658749512490971 1103 44-45 -0.29677897378779505 1103 46-47 -0.2286215367715556 1103 48-49 -0.30490207210468867 1103 50-51 -0.38597561131987845 1103 52-53 -0.18419813210025637 1103 54-55 -0.04654855552947623 1103 56-57 0.01632304851312938 1103 58-59 0.0015694193664828049 1103 60-61 -0.029154215066000688 1103 62-63 -0.06393677395419672 1103 64-65 -0.20096076576294308 1103 66-67 -0.02384854558886218 1103 68-69 -0.047084306460149605 1103 70-71 -0.140659522340691 1103 72-73 -0.08697766714854538 1103 74-75 -0.057803506879793076 1103 76-77 0.029994305090809803 1103 78-79 -0.1536322792593623 1103 80-81 -8.646892995223254E-4 1103 82-83 0.05717686395452404 1103 84-85 -0.008781609926227674 1103 86-87 -0.7492165607144692 1103 88-89 -0.20780905721436937 1103 90-91 -0.576433859872715 1103 92-93 -0.18622742284052407 1103 94-95 -0.6031575689661395 1103 96-97 -0.3309300380849436 1103 98-99 -0.31692228467735717 1103 100 -0.3482464977297397 1104 1 0.1308894941917309 1104 2 0.023995852835135167 1104 3 0.11846310397858772 1104 4 0.11164578106341594 1104 5 0.08456907478495168 1104 6 0.14357902694818847 1104 7 0.035301923240567135 1104 8 0.1762644423445039 1104 9 0.14443061537194524 1104 10-11 0.019103311611466722 1104 12-13 0.13353301308215038 1104 14-15 -0.008500939646872041 1104 16-17 0.11945782470576205 1104 18-19 0.12986158596361008 1104 20-21 0.11050410508110531 1104 22-23 -0.0333065536290178 1104 24-25 0.013916711951317495 1104 26-27 0.07575596962553277 1104 28-29 0.1077927992159502 1104 30-31 0.05721995306129202 1104 32-33 0.002591496863026066 1104 34-35 -0.04532782046960904 1104 36-37 -0.03710229092550321 1104 38-39 -0.07674411778035051 1104 40-41 0.060820486402761276 1104 42-43 -0.1420885295839227 1104 44-45 0.020559917019120633 1104 46-47 0.023210785519090393 1104 48-49 -0.0055191924781041735 1104 50-51 -0.2474891784666866 1104 52-53 -0.20974571152794397 1104 54-55 0.07063697126269375 1104 56-57 0.036713578003862324 1104 58-59 -0.04099367886811223 1104 60-61 -0.07482696879621642 1104 62-63 -0.008888897179836874 1104 64-65 -0.004664025196163379 1104 66-67 0.09250185662742183 1104 68-69 0.0014441066522010715 1104 70-71 0.011317950018188583 1104 72-73 0.03149317571220678 1104 74-75 0.06465129510677059 1104 76-77 0.017228970910124985 1104 78-79 0.03176000466072537 1104 80-81 0.01856132420003931 1104 82-83 0.10815999262243992 1104 84-85 -0.006555861505756155 1104 86-87 -0.11626460819006113 1104 88-89 0.026780969920167763 1104 90-91 -0.1361890902689602 1104 92-93 -0.13139904480475906 1104 94-95 -0.133460069078982 1104 96-97 -0.18298077572555016 1104 98-99 -0.1896225297092542 1104 100 -0.19411795874023596 1105 1 0.12231610246463731 1105 2 0.11541817546315158 1105 3 0.08064970305895969 1105 4 0.17372071982656934 1105 5 0.12672814297598478 1105 6 0.12401698482322132 1105 7 0.03865500245890274 1105 8 0.036495140827547345 1105 9 0.1612195416392339 1105 10-11 0.13313787025777657 1105 12-13 0.18665524551310853 1105 14-15 0.17866864530652293 1105 16-17 0.19665060936133472 1105 18-19 0.13394830452823214 1105 20-21 0.12440608899603944 1105 22-23 0.21304440075023479 1105 24-25 0.144611423161912 1105 26-27 0.12332551851878293 1105 28-29 0.06945504906040156 1105 30-31 0.01444396220151134 1105 32-33 0.1032752499015146 1105 34-35 0.1260921362955969 1105 36-37 0.04661938761796591 1105 38-39 0.050841807648026816 1105 40-41 -0.03422498697533172 1105 42-43 0.09118055216235632 1105 44-45 9.03724997940003E-4 1105 46-47 -0.06239640161248161 1105 48-49 -0.09940911819983 1105 50-51 -0.19386189387931552 1105 52-53 -0.0485548709436614 1105 54-55 0.07748962401849724 1105 56-57 0.05420540258156592 1105 58-59 0.05161321001994423 1105 60-61 0.06347738952946713 1105 62-63 0.035966565651129656 1105 64-65 0.08488449554402422 1105 66-67 0.1568835992196682 1105 68-69 0.14252803265792124 1105 70-71 0.05346834766064745 1105 72-73 -0.008751803193547403 1105 74-75 0.09372989196561576 1105 76-77 0.13560061386055366 1105 78-79 0.09929441126589467 1105 80-81 0.07036347290794609 1105 82-83 0.06683399774559717 1105 84-85 0.057003346721934633 1105 86-87 -0.033185104923159514 1105 88-89 -0.07879959950307835 1105 90-91 -0.04042183021369539 1105 92-93 -0.04132302190189563 1105 94-95 0.024974091738034332 1105 96-97 -0.020640839307237258 1105 98-99 -0.06768081027582795 1105 100 -0.010418344845309235 1106 1 -0.03394026416683005 1106 2 -0.010047480784830753 1106 3 0.03924183283975324 1106 4 0.009519197607716023 1106 5 7.78529766819247E-4 1106 6 0.12777478847903723 1106 7 0.12672467097841889 1106 8 -1.1144312628721948 1106 9 0.026012156155076127 1106 10-11 0.00686043184614249 1106 12-13 0.026923803008408242 1106 14-15 -0.06628693723914836 1106 16-17 -0.1348296009525498 1106 18-19 -0.03006933807444767 1106 20-21 -0.11645571197035309 1106 22-23 -0.08435693516320697 1106 24-25 0.006358552989325972 1106 26-27 -0.03411097593247092 1106 28-29 -0.1029296452900823 1106 30-31 -0.13658138978577483 1106 32-33 -0.04670350725422168 1106 34-35 -0.055674552052536797 1106 36-37 0.08008679364012039 1106 38-39 0.10630323821199283 1106 40-41 -0.05149549660391983 1106 42-43 -0.04819253048214023 1106 44-45 -0.1144472793915412 1106 46-47 0.05261563552250692 1106 48-49 -0.07533420459149198 1106 50-51 0.014893347533629253 1106 52-53 -0.02807464935899162 1106 54-55 -0.016232654942214708 1106 56-57 -0.03150994605138635 1106 58-59 -0.04192195684223066 1106 60-61 0.035461164527639255 1106 62-63 0.004214277582825332 1106 64-65 -0.12426015987249173 1106 66-67 0.0172956995282334 1106 68-69 0.06873885618743714 1106 70-71 0.07020790060661142 1106 72-73 -0.021220503181247352 1106 74-75 0.03586712749063281 1106 76-77 0.027875192698139983 1106 78-79 -0.03481576100138284 1106 80-81 -0.01694750625558683 1106 82-83 0.0606536344981059 1106 84-85 -0.012154883976268849 1106 86-87 -0.0752726403166939 1106 88-89 0.030166551371920036 1106 90-91 0.011951278119031628 1106 92-93 0.00449228080691455 1106 94-95 -0.01906363173162262 1106 96-97 -0.05193608869668509 1106 98-99 -0.06740882406073467 1106 100 -0.10472149802601294 1107 1 -0.013189307390234717 1107 2 -0.05322134541012957 1107 3 -0.14277300405480986 1107 4 0.15571392540841344 1107 5 0.06199638961194154 1107 6 0.02682524010795362 1107 7 0.09096603342231191 1107 8 0.17700164614843317 1107 9 0.12126227515913968 1107 10-11 0.08814137390982779 1107 12-13 0.11999556392702715 1107 14-15 0.02506149298248772 1107 16-17 0.08298724572202332 1107 18-19 0.06170444758666349 1107 20-21 0.0018124358479383318 1107 22-23 0.05611153775843292 1107 24-25 0.07278491808011722 1107 26-27 0.0963759626020817 1107 28-29 0.088462648812758 1107 30-31 0.007287511493004217 1107 32-33 0.07510660009268122 1107 34-35 0.04974459240396101 1107 36-37 -0.06677993767612378 1107 38-39 -0.10350469802230577 1107 40-41 -0.06776676538221693 1107 42-43 -0.03693687052911798 1107 44-45 -0.06128150094738771 1107 46-47 -0.029449169279196497 1107 48-49 0.10412047788932455 1107 50-51 -0.03367795099711657 1107 52-53 -0.044376438267001106 1107 54-55 -0.05251104038796228 1107 56-57 -0.08390375066344546 1107 58-59 0.03540591681415606 1107 60-61 -0.01912612166324834 1107 62-63 0.09351623463187053 1107 64-65 0.11954789090931683 1107 66-67 0.09424920921952662 1107 68-69 0.013078980793522987 1107 70-71 0.052189451439780044 1107 72-73 0.02429971681440435 1107 74-75 0.053915478621576085 1107 76-77 0.02476620785947148 1107 78-79 0.051579883271511306 1107 80-81 0.0604294181226912 1107 82-83 0.07094323099525113 1107 84-85 0.03270983608786793 1107 86-87 0.03907964403810027 1107 88-89 0.02605920033056819 1107 90-91 0.07680699941708724 1107 92-93 0.18694729473950744 1107 94-95 0.08767466374831301 1107 96-97 0.13013037221379875 1107 98-99 -0.044214273026650375 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-0.3297689663392447 2316 80-81 -0.15810664301406874 2316 82-83 -0.13335754997951454 2316 84-85 0.09693906333554025 2316 86-87 0.11398083298524497 2316 88-89 0.095599230793745 2316 90-91 0.2228631185103005 2316 92-93 0.269862493545137 2316 94-95 0.2798519200296248 2316 96-97 0.34458457197306913 2316 98-99 0.3571172707148982 2316 100 0.03980292626681603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 10846.0 1 998.0 2 597.0 3 455.0 4 423.0 5 387.0 6 406.0 7 861.0 8 1777.0 9 2870.0 10 4760.0 11 8109.0 12 12092.0 13 15902.0 14 18817.0 15 21927.0 16 25785.0 17 30838.0 18 37181.0 19 44153.0 20 51689.0 21 59441.0 22 67101.0 23 73810.0 24 77421.0 25 81693.0 26 88636.0 27 98899.0 28 114807.0 29 134861.0 30 163064.0 31 201598.0 32 255836.0 33 339428.0 34 487560.0 35 782498.0 36 849076.0 37 829613.0 38 774690.0 39 169997.0 40 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.28891734587254 17.580628604168098 12.323656928793245 23.806797121166124 2 16.339943373315187 20.04959930278668 39.82049519461486 23.78996212928328 3 19.368129316839696 26.187794705093133 29.15938786102904 25.28468811703813 4 12.995876284134036 15.91736088938402 36.658826040245025 34.42793678623693 5 13.460622136972795 36.31874431193636 33.71885811542105 16.501775435669792 6 30.865183024516398 34.819456302402024 19.40543717849559 14.90992349458599 7 28.29912127391992 31.548321833679978 21.95554524963395 18.197011642766153 8 27.023311976467788 33.629308606285214 20.649734747898698 18.6976446693483 9 27.93193962055753 15.635351618738733 20.115419968748302 36.317288791955434 10-11 25.57637015259232 25.33352412505655 28.1687470670413 20.921358655309835 12-13 25.919931295573406 23.301283811873873 28.021599570074184 22.757185322478534 14-15 23.4465324532366 24.577370765704234 26.22457009914459 25.751526681914576 16-17 22.93917728986277 27.002042032718926 26.91561946228862 23.143161215129687 18-19 22.701754092870708 26.402223398418144 28.525763289136545 22.3702592195746 20-21 23.524599313966686 25.78435961153298 28.400780282106798 22.29026079239354 22-23 23.268543531705223 25.61335507026833 28.121227369065632 22.99687402896081 24-25 22.931109890951724 25.71949656838609 28.24353068980215 23.10586285086004 26-27 22.83901652632904 25.620551489583427 28.41222747890717 23.12820450518036 28-29 22.82598322808593 26.039001150815178 28.3522839407577 22.782731680341193 30-31 23.506176922307912 25.76191884266069 28.243747888263236 22.48815634676816 32-33 23.10060500618253 25.89102463772824 28.26162288130767 22.746747474781564 34-35 23.277114036146678 26.041410155208993 28.18106053953012 22.50041526911421 36-37 23.297504889148367 25.952375729575678 28.027526031867627 22.722593349408324 38-39 23.2517756127333 26.249944210877736 27.832549782669204 22.66573039371976 40-41 23.23794509842705 26.016296454688653 28.01115694769687 22.73460149918742 42-43 23.020403837929237 26.02092803870024 27.785492623432912 23.173175499937607 44-45 23.008581548329776 26.141260893545304 27.779403102976385 23.07075445514853 46-47 22.99080126336999 26.03127987201963 27.715134852910396 23.26278401169998 48-49 23.00732448001625 26.10976241294935 27.71714213831214 23.165770968722256 50-51 22.790701676844304 26.474374976641478 27.68654609185291 23.048377254661307 52-53 22.932577999104574 26.54920273244728 27.24988216536271 23.268337103085436 54-55 22.774266828576323 26.374137885931326 27.544140493475656 23.307454792016696 56-57 22.96783822009635 26.37081657928586 27.2787691512148 23.38257604940299 58-59 22.918751249906126 26.36007928518449 27.56209027338875 23.159079191520636 60-61 23.219099801716546 26.493624144568827 27.551116222890414 22.736159830824214 62-63 22.954582546907492 26.442093882325658 27.616049112466083 22.987274458300767 64-65 22.831281376260797 26.344916287357755 27.488332142547637 23.33547019383381 66-67 22.925610305231938 26.304449164127735 27.265459231860294 23.504481298780032 68-69 22.69607795977551 26.31237785450612 27.44718428422635 23.54435990149202 70-71 22.760701091795777 26.13070166323983 27.509738816303862 23.598858428660527 72-73 22.684874954773893 26.450810456900598 27.242062617750378 23.62225197057513 74-75 22.72788807216986 26.521299081773442 27.132035030280647 23.61877781577605 76-77 22.518638473617504 26.26232627258041 27.36231227969433 23.85672297410776 78-79 22.625854688430046 26.195918188914174 27.375626424093912 23.802600698561868 80-81 22.642313781206287 26.394202805117708 27.05013890744148 23.913344506234527 82-83 22.733350059711142 26.397734866995336 27.121399661944885 23.747515411348637 84-85 22.732613755656324 26.533387304753802 26.858203315494965 23.87579562409491 86-87 22.494627492654367 26.47104247234585 27.12035969299572 23.913970342004063 88-89 22.45604123108523 26.235577070779332 27.17287967706008 24.13550202107536 90-91 22.890302667605937 26.14663642035798 27.09748948829933 23.86557142373676 92-93 22.752678177181465 26.204052213559564 27.09304697031562 23.950222638943348 94-95 22.5887565300071 26.028339500784973 27.186781201276556 24.196122767931367 96-97 22.513884135774 25.967479832609712 27.288060423893924 24.23057560772237 98-99 22.60870232958909 25.935122006666578 27.025687359451645 24.430488304292687 100 22.623471737773016 25.808550250195516 27.105080092444812 24.462897919586656 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5028.0 1 5575.0 2 6208.0 3 4935.5 4 2718.0 5 2021.5 6 2667.0 7 4607.5 8 7120.5 9 8206.0 10 7281.0 11 6160.5 12 6084.5 13 6366.5 14 6789.5 15 7257.5 16 7667.5 17 8098.0 18 8705.5 19 9255.5 20 9866.0 21 10888.5 22 12309.0 23 14214.5 24 16707.0 25 19691.0 26 24144.5 27 29502.5 28 34816.5 29 41547.5 30 49787.0 31 58004.0 32 67642.5 33 79272.5 34 91450.5 35 103841.0 36 119938.0 37 134749.5 38 143608.0 39 152586.0 40 159377.5 41 164436.0 42 170207.5 43 177148.5 44 186012.5 45 195883.0 46 205463.5 47 218160.5 48 235599.0 49 246503.5 50 256226.5 51 253460.0 52 237224.0 53 229290.5 54 223154.5 55 210839.5 56 193186.0 57 175497.5 58 159490.0 59 141863.0 60 120198.5 61 98141.0 62 80068.5 63 63698.5 64 48144.5 65 36351.0 66 27366.0 67 21250.0 68 16666.0 69 12689.0 70 10110.0 71 8095.5 72 6324.5 73 4993.0 74 3943.5 75 2913.0 76 2022.0 77 1347.5 78 860.5 79 569.0 80 407.5 81 283.0 82 195.5 83 136.0 84 101.5 85 80.5 86 69.0 87 55.0 88 37.5 89 35.5 90 30.0 91 22.0 92 18.5 93 16.5 94 13.0 95 8.5 96 9.0 97 9.0 98 6.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14433315222257906 2 0.07303341661734933 3 0.3142507232647873 4 0.3121467414539625 5 0.31351012166737696 6 0.2634690182787207 7 0.26459675252932274 8 0.2629303989351496 9 0.27466220151230847 10-11 0.25135008304837003 12-13 0.2599259129092918 14-15 0.23504843197809971 16-17 0.2427742531874483 18-19 0.2563912234671062 20-21 0.25770410811706085 22-23 0.24906936676543598 24-25 0.2482698536773226 26-27 0.2473272698260731 28-29 0.25780509924398043 30-31 0.2426900939150153 32-33 0.25489318841779895 34-35 0.23962669639845444 36-37 0.24786588916964422 38-39 0.2488000570936504 40-41 0.26660815914047126 42-43 0.2516614723563721 44-45 0.237278652697574 46-47 0.24018214759651216 48-49 0.24953224276381744 50-51 0.24368317332972458 52-53 0.2598670014185887 54-55 0.26932650364005684 56-57 0.2660527079424135 58-59 0.26377199165947945 60-61 0.24405347412842973 62-63 0.2710433527976899 64-65 0.2652111152180836 66-67 0.27011760080092695 68-69 0.2746453696578219 70-71 0.2950034976593623 72-73 0.30463973435293973 74-75 0.28395338518891067 76-77 0.28070483727299717 78-79 0.2779864927734116 80-81 0.2653962656174362 82-83 0.2750745819472301 84-85 0.2860237032907622 86-87 0.2778602538647621 88-89 0.28684846416060555 90-91 0.28019146571115594 92-93 0.2607338419246485 94-95 0.2816810848332199 96-97 0.2800568108752632 98-99 0.26739925630134137 100 0.28864947259067153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5941116.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.32620834994991 #Duplication Level Percentage of deduplicated Percentage of total 1 76.20585048448727 45.9721001381231 2 14.81036150636248 17.86905907941802 3 4.3719674174935275 7.91232651980721 4 1.7137207930791487 4.135291104677365 5 0.8424949193199603 2.541226201833508 6 0.4833935174481755 1.749677882915627 7 0.3178993767235518 1.342436482418094 8 0.22879532614611559 1.1041883611668248 9 0.16082521505583536 0.8731777888245442 >10 0.7816364393473237 8.43984136530129 >50 0.05197571217210606 2.148420976139722 >100 0.028491229746657926 3.1047960958706966 >500 0.0018080991566518769 0.7405601133590365 >1k 5.672461536121507E-4 0.6583035881122155 >5k 1.0635865380189573E-4 0.4081140048191258 >10k+ 1.0635865380189573E-4 1.000480297213808 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19901 0.3349707361377896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16329 0.27484735191166104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10549 0.1775592329791238 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7063 0.11888338823884267 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6287 0.10582186915724251 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.6831854486598142E-5 5 0.0 1.6831854486598142E-5 0.0 0.0 1.6831854486598142E-5 6 0.0 1.6831854486598142E-5 0.0 0.0 5.0495563459794424E-5 7 0.0 1.6831854486598142E-5 0.0 0.0 5.0495563459794424E-5 8 0.0 1.6831854486598142E-5 0.0 0.0 5.0495563459794424E-5 9 0.0 1.6831854486598142E-5 0.0 0.0 6.732741794639257E-5 10-11 0.0 3.3663708973196285E-5 0.0 8.415927243299071E-6 6.732741794639257E-5 12-13 0.0 3.3663708973196285E-5 0.0 1.6831854486598142E-5 7.574334518969163E-5 14-15 0.0 3.3663708973196285E-5 0.0 1.6831854486598142E-5 8.415927243299071E-5 16-17 0.0 3.3663708973196285E-5 0.0 3.3663708973196285E-5 1.1782298140618699E-4 18-19 0.0 3.3663708973196285E-5 0.0 4.2079636216495355E-5 1.5148669037938327E-4 20-21 0.0 5.0495563459794424E-5 0.0 6.732741794639257E-5 1.5148669037938327E-4 22-23 0.0 5.0495563459794424E-5 0.0 1.6831854486598142E-4 2.4406189005567305E-4 24-25 0.0 5.0495563459794424E-5 0.0 5.217874890845423E-4 3.3663708973196284E-4 26-27 0.0 5.0495563459794424E-5 0.0 0.0011782298140618698 3.53468944218561E-4 28-29 0.0 5.0495563459794424E-5 0.0 0.0031223090072639553 3.703007987051591E-4 30-31 0.0 5.0495563459794424E-5 0.0 0.009829803020173314 3.703007987051591E-4 32-33 0.0 5.0495563459794424E-5 0.0 0.028260683682998278 4.2079636216495355E-4 34-35 0.0 5.0495563459794424E-5 0.0 0.05803623426979039 4.2079636216495355E-4 36-37 0.0 5.0495563459794424E-5 0.0 0.10672237337227551 4.460441438948507E-4 38-39 0.0 5.0495563459794424E-5 0.0 0.18267611674304962 5.133715618412433E-4 40-41 0.0 5.0495563459794424E-5 0.0 0.2711527598518527 5.217874890845424E-4 42-43 0.0 5.0495563459794424E-5 0.0 0.35455459883294654 5.386193435711405E-4 44-45 0.0 5.0495563459794424E-5 0.0 0.4606036980257581 5.554511980577386E-4 46-47 0.0 5.0495563459794424E-5 0.0 0.5878188542354669 5.97530834274234E-4 48-49 0.0 5.0495563459794424E-5 0.0 0.720841000243052 7.406015974103182E-4 50-51 1.6831854486598142E-5 6.732741794639257E-5 0.0 0.8593166671042949 7.406015974103182E-4 52-53 1.6831854486598142E-5 6.732741794639257E-5 0.0 1.0038013060172535 7.995130881134117E-4 54-55 1.6831854486598142E-5 7.574334518969163E-5 0.0 1.172549063172643 8.752564333031033E-4 56-57 1.6831854486598142E-5 8.415927243299071E-5 0.0 1.3689515572495135 9.005042150330005E-4 58-59 1.6831854486598142E-5 8.415927243299071E-5 0.0 1.5809403485809739 9.089201422762996E-4 60-61 1.6831854486598142E-5 8.415927243299071E-5 0.0 1.7969856168437042 9.089201422762996E-4 62-63 1.6831854486598142E-5 8.415927243299071E-5 0.0 2.025250811463705 9.762475602226922E-4 64-65 1.6831854486598142E-5 8.415927243299071E-5 0.0 2.258447739448279 9.846634874659914E-4 66-67 2.5247781729897215E-5 8.415927243299071E-5 0.0 2.5284811809767724 0.0010014953419525893 68-69 3.3663708973196285E-5 8.415927243299071E-5 0.0 2.8285426509093576 0.0010099112691958885 70-71 3.3663708973196285E-5 8.415927243299071E-5 0.0 3.1579420432120835 0.0010099112691958885 72-73 3.3663708973196285E-5 8.415927243299071E-5 0.0 3.467437767584407 0.0011782298140618698 74-75 3.3663708973196285E-5 8.415927243299071E-5 0.0 3.7871588435573385 0.0011782298140618698 76-77 4.2079636216495355E-5 8.415927243299071E-5 0.0 4.134627231651427 0.0011782298140618698 78-79 5.0495563459794424E-5 1.1782298140618699E-4 0.0 4.5237460436726025 0.0011782298140618698 80-81 5.0495563459794424E-5 1.1782298140618699E-4 0.0 4.928291250330746 0.0011782298140618698 82-83 5.0495563459794424E-5 1.1782298140618699E-4 0.0 5.341311295722891 0.0012455572320082624 84-85 5.0495563459794424E-5 1.1782298140618699E-4 0.0 5.756123933617859 0.0012455572320082624 86-87 9.257519967628979E-5 1.1782298140618699E-4 0.0 6.197354167129543 0.0012455572320082624 88 1.5148669037938327E-4 1.1782298140618699E-4 0.0 6.549830031933395 0.0012455572320082624 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 12540 0.0 57.96886 2 GTACCTG 13820 0.0 52.98749 1 ACCTGGG 13895 0.0 51.43738 3 CCTGGGG 12040 0.0 39.32422 4 GTACCCG 1275 0.0 29.87911 1 CTGGGGG 7090 0.0 28.71264 5 TACCCGG 1540 0.0 25.036633 2 TGGGGGG 9785 0.0 23.676615 6 CATGGGG 15010 0.0 23.117071 4 ATGGGGG 9085 0.0 22.148806 5 GTACATG 29960 0.0 21.412401 1 GTATCAA 33645 0.0 21.177998 1 TACATGG 30435 0.0 20.887487 2 GTACCGG 960 0.0 20.086554 1 ACATGGG 30400 0.0 19.999483 3 GTACACG 1350 0.0 19.857925 1 TAACGCA 765 0.0 19.667343 4 TATCACG 575 0.0 19.625748 2 GAGTACT 17845 0.0 19.444958 12-13 ATAACGC 785 0.0 19.167883 3 >>END_MODULE