FastQCFastQC Report
Mon 6 Jun 2016
ERR523072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5941116
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183020.30805660081371916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163480.2751671571469064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103030.17341859677542065No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72140.12142499826631899No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG124050.059.5208052
GTACCTG138000.053.7973181
ACCTGGG139150.052.1775253
CCTGGGG119200.040.6462634
CTGGGGG69400.031.0807465
GTACCCG12900.029.886961
TATAACG6950.025.697342
ATAACGC7300.025.106143
TGGGGGG88800.025.0316836
TACCCGG14850.024.6863882
TACCGGG8400.024.0590552
GTACCGG9700.023.7511
TATCACG4800.023.4995422
CATGGGG150400.022.1532084
TAACGCA7850.022.1498244
ATGGGGG84900.021.5871415
GGTATCA248000.021.2715321
GTACATG292550.020.876931
GTATCAA319900.020.5470981
TACATGG299100.020.0818542
Produced by FastQC (version 0.11.5)