FastQCFastQC Report
Mon 6 Jun 2016
ERR523071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069936
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT135480.6545129897735968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77810.3759053420009121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63540.30696601247574806No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36700.1773001677346546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35070.16942552813227074No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20810.10053450927951396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG26300.043.9880262
ACCTGGG27350.040.9236833
GTACCTG39400.029.6012821
CCTGGGG26650.027.5284964
CATGGGG53850.025.2387144
TATAACG1701.2377495E-724.8970952
GTACATG94050.024.3014721
TATCACG1802.1503183E-723.5139242
TACATGG95950.023.2811132
ACATGGG94100.023.1890753
TAACGCA2253.1614036E-922.991394
ATGGGGG34300.022.8958325
GAGTACT64950.020.15245812-13
TACCGGG2351.2588316E-720.0118482
TGGGGGG35800.019.9579566
ATAACGC2601.6732884E-819.8963953
CGTATCA7100.019.19416294
GGCGTAA1250.0064079518.8024966
GTATAGG5300.018.6336751
GTACTAA3601.10958354E-1018.2886071
Produced by FastQC (version 0.11.5)