FastQCFastQC Report
Mon 6 Jun 2016
ERR523071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069936
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138610.6696342302370701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72410.3498175789009902No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT63570.30711094449296983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61980.29942954758021506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.1630002087021048No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT31540.15237186077250697RNA PCR Primer, Index 27 (95% over 23bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29650.1432411436875343No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26710.12903780599979903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG26000.040.1356472
ACCTGGG29000.035.6560633
TATAACG2151.8189894E-1228.422062
GTACCTG40250.026.880541
TAACGCA2305.456968E-1226.565884
ATAACGC2500.026.3206583
CCTGGGG27500.024.953354
ATGGGGG32000.023.6480355
GTACATG90750.023.3260881
CATGGGG51250.023.294244
TGGGGGG29450.023.1403776
ACATGGG89500.022.4240233
TACATGG94500.022.3339312
GAGTACT63400.021.23794712-13
GTATACG1150.003939771620.4525851
ACCGTAT1656.8647234E-519.9384448
GAGTACG3404.9112714E-1119.3698021
GTACTTT68700.019.05222514-15
AGTACTT65100.018.37316512-13
GTATAGA5150.018.2683281
Produced by FastQC (version 0.11.5)