FastQCFastQC Report
Mon 6 Jun 2016
ERR523070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7438178
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT576660.7752705030721233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222750.2994684988716323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173820.2336862602642744No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT165510.2225141694646189No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC122020.1640455498644964No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC116660.15683948407795564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100650.13531539578644125No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG96060.1291445297490864No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA94630.12722201592916976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG268150.028.685251
TACATGG273900.027.7208922
ACATGGG281750.026.3652343
CATGGGG189350.023.8844874
ATGGGGG107250.020.5581825
GAGTACT205250.020.1770712-13
AGAGTAC316000.019.34795410-11
GTACTTT213650.019.04370914-15
TACCTGG51750.018.805582
GAGTACA207900.018.7931541
CATGGGA96600.017.9578954
AGTACTT217900.017.87377412-13
AGTACAT203100.017.7083572
GTATAGA23350.016.5112551
TATACCG5700.016.4954555
GTATAGG18900.016.16981
GTACCTG64950.016.14281
ACTTTTT264700.015.71604816-17
TACTTTT249050.015.39359914-15
ATGGGGA80600.015.048525
Produced by FastQC (version 0.11.5)