FastQCFastQC Report
Mon 6 Jun 2016
ERR523070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7438178
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT592780.7969424770420928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202320.2720020951367391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171480.23054032855895626No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT164700.22142519310508565No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC130600.1755806327839963No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC122200.16428754461105932No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG99570.13386342730706363No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA97970.1317123628931709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96490.12972262831032008No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA81490.10955639943007549No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC76210.1024578868642294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG265450.026.5426311
TACATGG271100.025.4163192
ACATGGG274300.024.46493
CATGGGG181900.021.5205484
GAGTACT199500.019.6067412-13
ATGGGGG101950.019.5903765
TACCTGG50650.018.8375642
AGAGTAC315950.018.80835510-11
GTATAGG21100.018.5016541
GTACTTT209600.018.46794114-15
GAGTACA208000.018.1127381
AGTACTT210950.017.59533912-13
AGTACAT203550.017.2025722
CATGGGA97650.016.9399244
GTACCTG63900.015.3100761
ACTTTTT260650.014.98320816-17
TACTTTT247050.014.81221214-15
TATACTG30500.014.7915085
TGGGGGG88100.013.9221296
CATGGGT47850.013.6512984
Produced by FastQC (version 0.11.5)