FastQCFastQC Report
Mon 6 Jun 2016
ERR523069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456456
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT202470.31359309193774415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87330.13525996305093693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.11546582211665347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG86800.042.3723832
ACCTGGG97300.036.9778023
GTACCTG115650.031.964611
GTACATG218500.027.5733931
TACATGG225400.026.5834522
ACATGGG222750.025.590423
CCTGGGG95250.025.033724
CATGGGG141600.024.3789634
ATGGGGG86450.022.6294355
CCGTATC24000.021.14483894
TGGGGGG92100.020.1604866
GCCGTAT30100.019.35740394
CTGGGGG64300.018.0647245
GGGTTAG21350.017.8434851
GAGTACG8050.017.5274161
GAGTACT114750.017.36658512-13
TCGCCGT47050.016.9777994
CGTATCA18150.016.82889292-93
GTACCGG10600.016.4167961
GGTCGCC83600.016.35605494
Produced by FastQC (version 0.11.5)