FastQCFastQC Report
Mon 6 Jun 2016
ERR523069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456456
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT211250.3271918835968215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.13165117209812938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80230.12426321808744613No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT64660.10014782103370642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84350.043.4660262
ACCTGGG98250.036.2145923
GTACCTG122000.030.531021
CCTGGGG83450.027.2045174
GTACATG221250.025.1039811
TACATGG225250.024.0396482
TGGGGGG78150.023.6347056
ACATGGG224400.023.061353
ATGGGGG84500.022.972965
CATGGGG139300.022.775764
CTGGGGG55950.017.8938245
TATACCG4900.017.2663295
GAGTACT118750.016.919312-13
TATACTG29500.016.5704755
TATCTAA19950.016.4928462
GTACTTT120500.016.36131114-15
GTATTAC12350.015.9940441
GTGTAGC31350.015.7517111
ATAGGAC17750.015.6233413
GTATCGC4351.6498234E-915.1260466
Produced by FastQC (version 0.11.5)