FastQCFastQC Report
Mon 6 Jun 2016
ERR523068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6321182
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215360.3406957749357636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170880.27032918843342907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102110.16153624432898783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98400.049.8297541
TACCTGG73200.041.8678362
GTATCAA177300.041.0049061
GTACCTG89200.034.8700641
ACCTGGG90000.033.8393023
TCAACGC216350.032.973564
ATCAACG219350.032.482573
CAACGCA220550.032.302255
TATCAAC226300.031.6343782
AACGCAG231000.031.0134746
GTACATG201350.027.6470781
ACGCAGA259600.027.4704997
TACATGG203800.026.8929422
CGCAGAG268300.026.6491348
ACATGGG213150.025.2469543
CCTGGGG87850.024.2865074
GCAGAGT299150.023.4756289
CATGGGG153200.023.3747624
TAACGCA7350.021.7391514
ATAACGC7700.020.7528153
Produced by FastQC (version 0.11.5)