FastQCFastQC Report
Mon 6 Jun 2016
ERR523068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6321182
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204320.32323068691899715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167960.26570979921160315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102210.16169444259000926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107450.049.0395131
TACCTGG75450.045.0659522
GTATCAA184350.039.7101021
GTACCTG91500.037.946331
ACCTGGG89400.037.427043
TCAACGC225300.032.2041554
ATCAACG230050.031.4383243
CAACGCA232150.031.2336735
TATCAAC235850.030.6882572
AACGCAG242600.030.0817476
ACGCAGA276450.026.381387
CCTGGGG88300.026.2900894
GTACATG208400.025.5780091
CGCAGAG286450.025.3341738
TATAACG6450.024.7906932
TACATGG213450.024.588812
ACATGGG215000.023.7593253
ATAACGC7550.023.6526413
GCAGAGT316700.022.572839
TATCACG6700.022.4618052
Produced by FastQC (version 0.11.5)